Zobrazeno 1 - 10
of 77
pro vyhledávání: '"Morten H H Nørholm"'
Autor:
Cristina Hernández-Rollán, Anja K. Ehrmann, Arsenios Vlassis, Vijayalakshmi Kandasamy, Morten H. H. Nørholm
Publikováno v:
BMC Biotechnology, Vol 24, Iss 1, Pp 1-12 (2024)
Abstract Thermostable DNA polymerases, such as Taq isolated from the thermophilic bacterium Thermus aquaticus, enable one-pot exponential DNA amplification known as polymerase chain reaction (PCR). However, properties other than thermostability - suc
Externí odkaz:
https://doaj.org/article/77f1412e4bc14b2b83ef41ab7b1ac3af
Autor:
Tanveer S. Batth, Jonas L. Simonsen, Cristina Hernández-Rollán, Søren Brander, Jens Preben Morth, Katja S. Johansen, Morten H. H. Nørholm, Jakob B. Hoof, Jesper V. Olsen
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes that help break down lignocellulose, making them highly attractive for improving biomass utilization in industrial biotechnology. The catalytically essential N-terminal histid
Externí odkaz:
https://doaj.org/article/39d0be4a617848e5b7b1a2db1d0b93fa
Autor:
Tobias Tandrup, Sebastian J. Muderspach, Sanchari Banerjee, Gianluca Santoni, Johan Ø. Ipsen, Cristina Hernández-Rollán, Morten H. H. Nørholm, Katja S. Johansen, Flora Meilleur, Leila Lo Leggio
Publikováno v:
IUCrJ, Vol 9, Iss 5, Pp 666-681 (2022)
The recently discovered lytic polysaccharide monooxygenases (LPMOs) are Cu-containing enzymes capable of degrading polysaccharide substrates oxidatively. The generally accepted first step in the LPMO reaction is the reduction of the active-site metal
Externí odkaz:
https://doaj.org/article/f55e8c4da97646a8aef9109ae3ae787c
Autor:
Andreas B. Bertelsen, Celeste Menuet Hackney, Carolyn N. Bayer, Lau D. Kjelgaard, Maja Rennig, Brian Christensen, Esben Skipper Sørensen, Helena Safavi‐Hemami, Tune Wulff, Lars Ellgaard, Morten H. H. Nørholm
Publikováno v:
Microbial Biotechnology, Vol 14, Iss 6, Pp 2566-2580 (2021)
Summary Secreted proteins and peptides hold large potential both as therapeutics and as enzyme catalysts in biotechnology. The high stability of many secreted proteins helps maintain functional integrity in changing chemical environments and is a con
Externí odkaz:
https://doaj.org/article/ae7d52884de74d71a1a0d3932c06a51c
Autor:
Ida Lauritsen, Pernille Ott Frendorf, Silvia Capucci, Sophia A. H. Heyde, Sarah D. Blomquist, Sofie Wendel, Emil C. Fischer, Agnieszka Sekowska, Antoine Danchin, Morten H. H. Nørholm
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Microbial evolution often involves transient phenotypes and sequential development of multiple mutations of unclear relevance. Here, the authors show that the evolution of non-growing E. coli cells can be driven by alterations in pyrimidine nucleosid
Externí odkaz:
https://doaj.org/article/ddb79980ec96494cbf407a7e2b45ecb8
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Genome engineering is challenging compared to plasmid DNA manipulation. Here the authors create a simple methodology called SEGA that enables genome engineering by combining DNA and bacterial cells followed by identification of recombinant clones by
Externí odkaz:
https://doaj.org/article/22f6fac5cbeb4aea9cfc13c6aad002c5
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
Heyde and Nørholm investigate the role of RNAse E in the toxicity mediated by overexpression of heterologous proteins in derivatives of the BL21(DE3) strain of E. coli. The authors report an unexpected connection between global RNA metabolism and th
Externí odkaz:
https://doaj.org/article/62bfa52235dd4d8f872de90d6665d3a4
Publikováno v:
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-9 (2021)
Abstract Poly(ethylene terephthalate) (PET) is the world’s most abundant polyester plastic, and its ongoing accumulation in nature is causing a global environmental problem. Currently, the main recycling processes utilize thermomechanical or chemic
Externí odkaz:
https://doaj.org/article/3530ff8c713445c0ab3cf241874d95dc
Autor:
Søren Brander, Stine Lausten, Johan Ø. Ipsen, Kristoffer B. Falkenberg, Andreas B. Bertelsen, Morten H. H. Nørholm, Lars H. Østergaard, Katja S. Johansen
Publikováno v:
Biotechnology for Biofuels, Vol 14, Iss 1, Pp 1-13 (2021)
Abstract Background Lytic polysaccharide monooxygenases (LPMOs) are important industrial enzymes known for their catalytic degradation of recalcitrant polymers such as cellulose or chitin. Their activity can be measured by lengthy HPLC methods, while
Externí odkaz:
https://doaj.org/article/05176937ee244f36be03805ec77ebf2c
Autor:
Kiavash Mirzadeh, Patrick J. Shilling, Rageia Elfageih, Alister J. Cumming, Huanhuan L. Cui, Maja Rennig, Morten H. H. Nørholm, Daniel O. Daley
Publikováno v:
Microbial Cell Factories, Vol 19, Iss 1, Pp 1-12 (2020)
Abstract Background Recombinant proteins are often engineered with an N-terminal signal peptide, which facilitates their secretion to the oxidising environment of the periplasm (gram-negative bacteria) or the culture supernatant (gram-positive bacter
Externí odkaz:
https://doaj.org/article/3d4522af475f4477a00ac0d684dd7b7e