Single-molecule Imaging Analysis of Binding, Processive Movement, and Dissociation of Cellobiohydrolase Trichoderma reesei Cel6A and Its Domains on Crystalline Cellulose

Autor: Masahiro Samejima, Tomoyuki Tasaki, Kiyohiko Igarashi, Hiroyuki Noji, Ryota Iino, Yasuko Okuni, Morice Maximilien, Taku Uchiyama, Akasit Visootsat, Daiki Ishiwata, Mayuko Yamamoto, Akihiko Nakamura

Publikováno v: Nakamura, A, Tasaki, T, Ishiwate, D, Yamamoto, M, Okuni, Y, Visootsat, A, Maximilien, M, Noji, H, Uchiyama, T, Samejima, M, Igarashi, K & Iino, R 2016, ' Single-molecule imaging analysis of binding, processive movement, and dissociation of cellobiohydrolase trichoderma reesei Cel6A and its domains on crystalline cellulose ', Journal of Biological Chemistry, vol. 291, no. 43, pp. 22404-22413 . https://doi.org/10.1074/jbc.M116.752048

Trichoderma reesei Cel6A (TrCel6A) is a cellobiohydrolase that hydrolyzes crystalline cellulose into cellobiose. Here we directly observed the reaction cycle (binding, surface movement, and dissociation) of single-molecule intact TrCel6A, isolated ca

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https://doi.org/10.1074/jbc.m116.752048