Enzymatic, structural, and biophysical characterization of a single-domain antibody (VHH) selectively and tightly inhibiting neutrophil elastase and exhibiting favorable developability properties.

Autor: Redeghieri P; Nano-Antibodies to Explore Protein Structure and Functions (NEPTUNS), Centre for Protein Engineering, InBios, Department of Life Sciences, University of Liège, Liège, Belgium., Moray J; Nano-Antibodies to Explore Protein Structure and Functions (NEPTUNS), Centre for Protein Engineering, InBios, Department of Life Sciences, University of Liège, Liège, Belgium., Kerff F; Biological Macromolecule Crystallography, Centre for Protein Engineering, InBios, Department of Life Sciences, University of Liège, Liège, Belgium., Gohy S; Department of Pneumology, ENT and Dermatology, Institute of Experimental and Clinical Research (IREC), Université Catholique de Louvain, Brussels, Belgium.; Cystic Fibrosis Reference Centre, Cliniques Universitaires Saint-Luc, Brussels, Belgium., Leal T; Louvain Center for Toxicology and Applied Pharmacology (LTAP), Institute of Experimental and Clinical Research (IREC), Université Catholique de Louvain, Brussels, Belgium., Muyldermans S; Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium., Vanbever R; Louvain Drug Research Institute (LDRI), Université Catholique de Louvain, Brussels, Belgium., Morales-Yánez FJ; Nano-Antibodies to Explore Protein Structure and Functions (NEPTUNS), Centre for Protein Engineering, InBios, Department of Life Sciences, University of Liège, Liège, Belgium., Dumoulin M; Nano-Antibodies to Explore Protein Structure and Functions (NEPTUNS), Centre for Protein Engineering, InBios, Department of Life Sciences, University of Liège, Liège, Belgium.

Publikováno v: Protein science : a publication of the Protein Society [Protein Sci] 2024 Dec; Vol. 33 (12), pp. e5227.