Mechanism of regulation of pantothenate biosynthesis by the PanD-PanZ.AcCoA complex reveals an additional mode of action for the antimetabolite N-pentyl pantothenamide (N5-Pan)

Autor: Arnott, ZLP, Nozaki, S, Monteiro, DCF, Morgan, HE, Pearson, AR, Niki, H, Webb, ME

The antimetabolite pentyl pantothenamide has broad spectrum antibiotic activity but exhibits enhanced activity against Escherichia coli. The PanDZ complex has been proposed to regulate the pantothenate biosynthetic pathway in E. coli by limiting the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=core_ac_uk__::2dfc20cb770602a4fc9cc585c1200583

Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.

Autor: Holmes S; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia., Kirkwood HJ; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Bean R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Giewekemeyer K; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Martin AV; School of Science, RMIT University, Melbourne, VIC, 3000, Australia., Hadian-Jazi M; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Australian Nuclear Science and Technology Organisation (ANSTO), Sydney, NSW, 2234, Australia., Wiedorn MO; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Oberthür D; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Marman H; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia., Adriano L; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Al-Qudami N; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Bajt S; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany., Barák I; Institute of Molecular Biology, SAS, Dubravska cesta 21, 845 51, Bratislava, Slovakia., Bari S; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Bielecki J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Brockhauser S; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Coleman MA; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA., Cruz-Mazo F; Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092, Sevilla, Spain.; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA., Danilevski C; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Dörner K; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Gañán-Calvo AM; Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092, Sevilla, Spain., Graceffa R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Fanghor H; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Max-Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 175, 22761, Hamburg, Germany.; University of Southampton, Southampton, SO17 1BJ, UK., Heymann M; Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Am Pfaffenwaldring 57, 70569, Stuttgart, Germany., Frank M; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA., Kaukher A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Kim Y; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Kobe B; School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, QLD, 4072, Australia., Knoška J; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Laurus T; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Letrun R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Maia L; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Messerschmidt M; School of Molecular Science, Arizona State University, Tempe, AZ, 85281, USA., Metz M; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Michelat T; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Mills G; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Molodtsov S; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger, Str. 23, 09599, Freiberg, Germany.; ITMO University, Kronverksky pr. 49, St. Petersburg, 197101, Russia., Monteiro DCF; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany.; Hauptman-Woodward Medical Research Institute, 700 Ellicott St., Buffalo, NY, 14203, USA., Morgan AJ; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; Department of Physics, University of Melbourne, Parkville, VIC, 3010, Australia., Münnich A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Peña Murillo GE; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Previtali G; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Round A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Sato T; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Schubert R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Schulz J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Shelby M; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA., Seuring C; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany., Sellberg JA; Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-106 91, Stockholm, Sweden., Sikorski M; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Silenzi A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Stern S; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Sztuk-Dambietz J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Szuba J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Trebbin M; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger, Str. 23, 09599, Freiberg, Germany.; Department of Chemistry, State University of New York at Buffalo, 760 Natural Sciences Complex, Buffalo, NY, 14260, USA., Vagovic P; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Ve T; Institute for Glycomics, Griffith University, Southport, QLD, 4222, Australia., Weinhausen B; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Wrona K; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Xavier PL; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; Max-Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 175, 22761, Hamburg, Germany., Xu C; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Yefanov O; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Nugent KA; Department of Quantum Science and Technology, Research School of Physics, Australian National University, Canberra, ACT, 2601, Australia., Chapman HN; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Mancuso AP; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia., Barty A; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Abbey B; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia. b.abbey@latrobe.edu.au.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia. b.abbey@latrobe.edu.au., Darmanin C; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia. c.darmanin@latrobe.edu.au.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia. c.darmanin@latrobe.edu.au.

Publikováno v: Nature communications [Nat Commun] 2022 Aug 11; Vol. 13 (1), pp. 4708. Date of Electronic Publication: 2022 Aug 11.

Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.

Autor: Gilbile D; Department of Chemical Engineering, University of California at Davis, Davis, CA 95616, USA. tlkuhl@ucdavis.edu., Shelby ML; Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA., Lyubimov AY; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Wierman JL; Lumen Bioscience, Seattle, WA 98103, USA., Monteiro DCF; Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, New York 14203, USA., Cohen AE; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Russi S; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Coleman MA; Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.; Department of Radiation Oncology, School of Medicine, University of California at Davis, Sacramento, CA 95817, USA., Frank M; Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.; Department of Biochemistry and Molecular Medicine, School of Medicine, University of California at Davis, Sacramento, CA 95817, USA., Kuhl TL; Department of Chemical Engineering, University of California at Davis, Davis, CA 95616, USA. tlkuhl@ucdavis.edu.

Publikováno v: Lab on a chip [Lab Chip] 2021 Dec 07; Vol. 21 (24), pp. 4831-4845. Date of Electronic Publication: 2021 Dec 07.

X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease.

Autor: Günther S; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany. sebastian.guenther@desy.de alke.meents@desy.de., Reinke PYA; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Fernández-García Y; Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany., Lieske J; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Lane TJ; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Ginn HM; Diamond Light Source Ltd., Diamond House, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK., Koua FHM; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Ehrt C; Universität Hamburg, Center for Bioinformatics, Bundesstr. 43, 20146 Hamburg, Germany., Ewert W; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Oberthuer D; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Yefanov O; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Meier S; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Institut für Nanostruktur- und Festkörperphysik, Luruper Chaussee 149, 22761 Hamburg, Germany., Lorenzen K; European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany., Krichel B; Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistr. 52, 20251 Hamburg, Germany., Kopicki JD; Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistr. 52, 20251 Hamburg, Germany., Gelisio L; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Brehm W; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Dunkel I; Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195 Berlin, Germany., Seychell B; Universität Hamburg, Department of Chemistry, Institute of Physical Chemistry, Grindelallee 117, 20146 Hamburg, Germany., Gieseler H; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Institut für Nanostruktur- und Festkörperphysik, Luruper Chaussee 149, 22761 Hamburg, Germany., Norton-Baker B; Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.; Department of Chemistry, UC Irvine, Irvine, CA 92697-2025, USA., Escudero-Pérez B; Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany., Domaracky M; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Saouane S; Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607, Hamburg, Germany., Tolstikova A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., White TA; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Hänle A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Groessler M; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Fleckenstein H; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Trost F; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Galchenkova M; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Gevorkov Y; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.; Vision Systems, Hamburg University of Technology, 21071 Hamburg, Germany., Li C; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Awel S; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Peck A; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Barthelmess M; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Schlünzen F; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Lourdu Xavier P; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.; Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany., Werner N; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., Andaleeb H; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., Ullah N; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., Falke S; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., Srinivasan V; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., França BA; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., Schwinzer M; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., Brognaro H; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., Rogers C; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Institut für Nanostruktur- und Festkörperphysik, Luruper Chaussee 149, 22761 Hamburg, Germany., Melo D; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Institut für Nanostruktur- und Festkörperphysik, Luruper Chaussee 149, 22761 Hamburg, Germany., Zaitseva-Kinneberg JI; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Institut für Nanostruktur- und Festkörperphysik, Luruper Chaussee 149, 22761 Hamburg, Germany., Knoska J; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Peña-Murillo GE; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Mashhour AR; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Hennicke V; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Fischer P; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Hakanpää J; Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607, Hamburg, Germany., Meyer J; Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607, Hamburg, Germany., Gribbon P; Fraunhofer Institute for Translational Medicine and Pharmacology and Fraunhofer Cluster of Excellence for Immune Mediated Diseases, Schnackenburgallee 114, 22525 Hamburg, Germany., Ellinger B; Fraunhofer Institute for Translational Medicine and Pharmacology and Fraunhofer Cluster of Excellence for Immune Mediated Diseases, Schnackenburgallee 114, 22525 Hamburg, Germany., Kuzikov M; Fraunhofer Institute for Translational Medicine and Pharmacology and Fraunhofer Cluster of Excellence for Immune Mediated Diseases, Schnackenburgallee 114, 22525 Hamburg, Germany., Wolf M; Fraunhofer Institute for Translational Medicine and Pharmacology and Fraunhofer Cluster of Excellence for Immune Mediated Diseases, Schnackenburgallee 114, 22525 Hamburg, Germany., Beccari AR; Dompé Farmaceutici SpA, 67100 L'Aquila, Italy., Bourenkov G; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., von Stetten D; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Pompidor G; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Bento I; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Panneerselvam S; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Karpics I; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Schneider TR; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Garcia-Alai MM; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Niebling S; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Günther C; EMBL Outstation Hamburg, c/o DESY, Notkestr. 85, 22607 Hamburg, Germany., Schmidt C; European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany., Schubert R; European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany., Han H; European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany., Boger J; Institute of Molecular Medicine, University of Lübeck, 23562 Lübeck, Germany., Monteiro DCF; Hauptmann Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, NY 14203, USA., Zhang L; Institute of Molecular Medicine, University of Lübeck, 23562 Lübeck, Germany.; German Center for Infection Research, Hamburg-Lübeck-Borstel-Riems Site, University of Lübeck, 23562 Lübeck, Germany., Sun X; Institute of Molecular Medicine, University of Lübeck, 23562 Lübeck, Germany.; German Center for Infection Research, Hamburg-Lübeck-Borstel-Riems Site, University of Lübeck, 23562 Lübeck, Germany., Pletzer-Zelgert J; Universität Hamburg, Center for Bioinformatics, Bundesstr. 43, 20146 Hamburg, Germany., Wollenhaupt J; Helmholtz Zentrum Berlin, Macromolecular Crystallography, Albert-Einstein-Str. 15, 12489 Berlin, Germany., Feiler CG; Helmholtz Zentrum Berlin, Macromolecular Crystallography, Albert-Einstein-Str. 15, 12489 Berlin, Germany., Weiss MS; Helmholtz Zentrum Berlin, Macromolecular Crystallography, Albert-Einstein-Str. 15, 12489 Berlin, Germany., Schulz EC; Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany., Mehrabi P; Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany., Karničar K; Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova 39, 1000 Ljubljana, Slovenia., Usenik A; Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova 39, 1000 Ljubljana, Slovenia., Loboda J; Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia., Tidow H; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany., Chari A; Research Group for Structural Biochemistry and Mechanisms, Department of Structural Dynamics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany., Hilgenfeld R; Institute of Molecular Medicine, University of Lübeck, 23562 Lübeck, Germany.; German Center for Infection Research, Hamburg-Lübeck-Borstel-Riems Site, University of Lübeck, 23562 Lübeck, Germany., Uetrecht C; Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistr. 52, 20251 Hamburg, Germany., Cox R; Institute for Organic Chemistry and BMWZ, Leibniz University of Hannover, Schneiderberg 38, 30167 Hannover, Germany., Zaliani A; Fraunhofer Institute for Translational Medicine and Pharmacology and Fraunhofer Cluster of Excellence for Immune Mediated Diseases, Schnackenburgallee 114, 22525 Hamburg, Germany., Beck T; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Department of Chemistry, Institute of Physical Chemistry, Grindelallee 117, 20146 Hamburg, Germany., Rarey M; Universität Hamburg, Center for Bioinformatics, Bundesstr. 43, 20146 Hamburg, Germany., Günther S; Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany., Turk D; Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova 39, 1000 Ljubljana, Slovenia., Hinrichs W; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany.; Universität Greifswald, Institute of Biochemistry, Felix-Hausdorff-Str. 4, 17489 Greifswald, Germany., Chapman HN; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Department of Physics, Luruper Chaussee 149, 22761 Hamburg, Germany., Pearson AR; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Institut für Nanostruktur- und Festkörperphysik, Luruper Chaussee 149, 22761 Hamburg, Germany., Betzel C; Hamburg Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.; Universität Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology and Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, 22607 Hamburg, Germany., Meents A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany. sebastian.guenther@desy.de alke.meents@desy.de.

Publikováno v: Science (New York, N.Y.) [Science] 2021 May 07; Vol. 372 (6542), pp. 642-646. Date of Electronic Publication: 2021 Apr 02.

Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales.

Autor: Klebl DP; School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK., Gravett MSC; School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK., Kontziampasis D; School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK; School of Chemical and Process Engineering, Faculty of Engineering and Physical Sciences, University of Leeds, Leeds LS2 9JT, UK; Institute of Business, Industry & Leadership, University of Cumbria, Carlisle CA1 2HH, UK., Wright DJ; School of Medicine, Faculty of Medicine and Health & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK., Bon RS; School of Medicine, Faculty of Medicine and Health & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK., Monteiro DCF; Hauptman-Woodward Medical Research Institute, Buffalo, NY, USA., Trebbin M; Hauptman-Woodward Medical Research Institute, Buffalo, NY, USA; Department of Chemistry, State University of New York at Buffalo, Buffalo, NY, USA., Sobott F; School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK; Department of Chemistry, Biomolecular & Analytical Mass Spectrometry Group, University of Antwerp, Antwerp, Belgium., White HD; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA, USA., Darrow MC; SPT Labtech, Melbourn Science Park, Melbourn, Cambridge, UK., Thompson RF; School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK. Electronic address: r.f.thompson@leeds.ac.uk., Muench SP; School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK. Electronic address: s.p.muench@leeds.ac.uk.

Publikováno v: Structure (London, England : 1993) [Structure] 2020 Nov 03; Vol. 28 (11), pp. 1238-1248.e4. Date of Electronic Publication: 2020 Aug 18.