The suppressor of AAC2 Lethality SAL1 modulates sensitivity of heterologously expressed artemia ADP/ATP carrier to bongkrekate in yeast.

Autor: Monika Wysocka-Kapcinska, Beata Torocsik, Lilla Turiak, George Tsaprailis, Cynthia L David, Andrea M Hunt, Karoly Vekey, Vera Adam-Vizi, Roza Kucharczyk, Christos Chinopoulos

Publikováno v: PLoS ONE, Vol 8, Iss 9, p e74187 (2013)

The ADP/ATP carrier protein (AAC) expressed in Artemia franciscana is refractory to bongkrekate. We generated two strains of Saccharomyces cerevisiae where AAC1 and AAC3 were inactivated and the AAC2 isoform was replaced with Artemia AAC containing a

Externí odkaz: https://doaj.org/article/f097ba8f46ff43ef9ec2d7360b8b2377

Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals

Autor: Zaneta Jastrzebska, Monika Wysocka-Kapcinska, Danuta Plochocka, Agnieszka Gornicka, Anita K. Hopper, Teresa Zoladek, Piotr Cholbinski

Publikováno v: European Journal of Cell Biology. 90:834-843

The Rsp5 ubiquitin ligase regulates numerous cellular processes. Rsp5 is mainly localized to the cytoplasm but nuclear localization was also reported. A potential nuclear export signal was tested for activity by using a GFP(2) reporter. The 687-LIGGI

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::903846c49f2eb117a8d843a94e13aa0a
https://doi.org/10.1016/j.ejcb.2011.06.008

Genetic engineering and molecular characterization of yeast strain expressing hybrid human-yeast squalene synthase as a tool for anti-cholesterol drug assessment

Autor: Agata Leszczynska, Beata Burzynska, Maciej Sojka, Joanna Komaszylo, Danuta Plochocka, Monika Gora, Suman Kapur, Witold Danikiewicz, Agata Maciejak, Monika Wysocka-Kapcinska, Ewa Swiezewska, Ilona Warchol, Dorota Tulacz

Publikováno v: Journal of applied microbiology. 120(4)

Aims The main objective of the study is molecular and biological characterization of the human-yeast hybrid squalene synthase, as a promising target for treatment of hypercholesterolemia. Methods and Results The human-yeast hybrid squalene synthase,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::684aa748d830b5b67f2d84f2015bb8d3
https://pubmed.ncbi.nlm.nih.gov/26757023

Defining the pathogenesis of human mtDNA mutations using a yeast model: The case of T8851C

Autor: Monika Wysocka-Kapcinska, Nadine Camougrand, Marie-France Giraud, Francis Haraux, Bénédicte Salin, Nahia Ezkurdia, Jean Velours, Jean-Paul di Rago, Roza Kucharczyk, Daniel Brèthes

Publikováno v: International Journal of Biochemistry and Cell Biology
International Journal of Biochemistry and Cell Biology, Elsevier, 2013, 45 (1), pp.130-40. ⟨10.1016/j.biocel.2012.07.001⟩

International audience; More and more mutations are found in the mitochondrial DNA of various patients but ascertaining their pathogenesis is often difficult. Due to the conservation of mitochondrial function from yeast to humans, the unique ability

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01e50989a87ea92c57a03b70caddd3fc
https://hal.archives-ouvertes.fr/hal-00735756

Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1

Autor: Joanna Kaminska, Teresa Żołądek, Magdalena Sędek, Monika Wysocka-Kapcinska

Publikováno v: FEBS letters. 581(28)

Pan1 is an actin patch-associated protein involved in endocytosis. Our studies revealed that in oleate-grown cells Pan1 is located in the nucleus as well as in patches. One of three putative nuclear localization signals (NLS) of Pan1, NLS2, directed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f34583db34d186da3e260af006da38e5
https://pubmed.ncbi.nlm.nih.gov/17967424