Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Monika G Wood"'
Autor:
Ce Shi, Michael P Killoran, Mary P Hall, Paul Otto, Monika G Wood, Ethan Strauss, Lance P Encell, Thomas Machleidt, Keith V Wood, Thomas A Kirkland
Publikováno v:
PLoS ONE, Vol 15, Iss 12, p e0243747 (2020)
Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings. Solutions of detection reagent, containing a mixture of a substrate and luciferase enzyme that
Externí odkaz:
https://doaj.org/article/4549be00272c490db5ba97c1d7c02863
Autor:
Stuart K Forsyth, Emily Jost, Monika G. Wood, Valerie T Ressler, Dan Lazar, Kris Zimmermann, Connor Fitzgerald, Thomas A. Kirkland, Mary P. Hall, Thomas Machleidt, Thomas P. Smith, Kincaid Virginia, Keith V. Wood, Robin Hurst, Lance P. Encell, Melanie Dart
Publikováno v:
Analytical Chemistry. 93:5177-5184
Enzyme-linked immunosorbent assays (ELISAs) are used extensively for the detection and quantification of biomolecules in clinical diagnostics as well as in basic research. Although broadly used, the inherent complexities of ELISAs preclude their util
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f25f1b91446dc05c2258e15579e5bb58
https://doi.org/10.1021/acs.analchem.0c05074
https://doi.org/10.1021/acs.analchem.0c05074
Autor:
Laura Mezzanotte, Clemens W G M Löwik, Lance P. Encell, Mary P. Hall, Yanto Ridwan, Natasa Gaspar, Thomas A. Kirkland, Giorgia Zambito, Ce Shi, Monika G. Wood, Fabio Stellari
Publikováno v:
iScience, Vol 24, Iss 1, Pp 101986-(2021)
iScience, 24(1):101986. Elsevier Inc.
iScience
iScience, 24(1):101986. Elsevier Inc.
iScience
Summary For in vivo multicolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c731d4b4f43a2205793c7291a1082b83
http://www.sciencedirect.com/science/article/pii/S2589004220311834
http://www.sciencedirect.com/science/article/pii/S2589004220311834
Autor:
Ethan Edward Strauss, Paul Otto, Monika G. Wood, Mary P. Hall, Michael P Killoran, Lance P. Encell, Ce Shi, Thomas Machleidt, Keith V. Wood, Thomas A. Kirkland
Publikováno v:
PLoS ONE
PLoS ONE, Vol 15, Iss 12, p e0243747 (2020)
PLoS ONE, Vol 15, Iss 12, p e0243747 (2020)
Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings. Solutions of detection reagent, containing a mixture of a substrate and luciferase enzyme that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ef2bf1e48d69be03ac6ff5852c2614a
https://pubmed.ncbi.nlm.nih.gov/33315907
https://pubmed.ncbi.nlm.nih.gov/33315907
Autor:
Paul Otto, Danette L. Daniels, Kate Zhao, Monika G. Wood, Mark McDougall, Dieter Klaubert, Georgyi V. Los, Sarah Wheeler, James Robert Hartnett, Natasha Karassina, Jami English, Chad Zimprich, Nancy Murphy, Jacqui Méndez, Robert F. Bulleit, Pete Stecha, Keith V. Wood, Robin Hurst, Kris Zimmerman, Randall D. Learish, Hélène A Benink, Gediminas Vidugiris, Marjeta Urh, Aldis Darzins, Michael R. Slater, Rachel Friedman Ohana, Lance P. Encell
Publikováno v:
Current Chemical Genomics
Our fundamental understanding of proteins and their biological significance has been enhanced by genetic fusion tags, as they provide a convenient method for introducing unique properties to proteins so that they can be examinedin isolation. Commonly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ba84480023d119ed3f0274fd4ecd399
https://doi.org/10.2174/1875397301206010055
https://doi.org/10.2174/1875397301206010055
Autor:
Kris Zimmerman, Melanie Dart, Sergiy Levin, Rachel Friedman Ohana, Lance P. Encell, Thomas A. Kirkland, H. Tetsuo Uyeda, Keith V. Wood, Robin Hurst, Monika G. Wood, Marie K. Schwinn
Publikováno v:
ACS chemical biology. 11(9)
The benefits provided by phenotypic screening of compound libraries are often countered by difficulties in identifying the underlying cellular targets. We recently described a new approach utilizing a chloroalkane capture tag, which can be chemically
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12d1aad67c06d9fbcdc376ca25271134
https://pubmed.ncbi.nlm.nih.gov/27414062
https://pubmed.ncbi.nlm.nih.gov/27414062
Autor:
Brock Binkowski, Mary P. Hall, Monika G. Wood, Kristopher Zimmerman, Paul Otto, Keith V. Wood, Gediminas Vidugiris, Dieter Klaubert, Hélène A Benink, Matthew B. Robers, James Unch, Lance P. Encell, Michael P. Valley, Frank Fan, Poncho Meisenheimer, Braeden L. Butler, Thomas Machleidt, Christopher T. Eggers, Michael R. Slater
Publikováno v:
ACS Chemical Biology
Bioluminescence methodologies have been extraordinarily useful due to their high sensitivity, broad dynamic range, and operational simplicity. These capabilities have been realized largely through incremental adaptations of native enzymes and substra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b14760e5049c02c7d69b1883d21b6ca
https://doi.org/10.1021/cb3002478
https://doi.org/10.1021/cb3002478
Publikováno v:
Expert Opinion on Drug Metabolism & Toxicology. 8:1115-1130
The cytochrome P450s (CYPs) are central to ADME studies because of their central role in drug metabolism. Proper CYP assay design and a correct understanding of CYP assay selectivity are critical for generating and interpreting biologically relevant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05bf2c3dc42a6b5b5594ea9e9b7beb6b
https://doi.org/10.1517/17425255.2012.695345
https://doi.org/10.1517/17425255.2012.695345
Autor:
Mei Cong, Matthew B. Robers, Monika G. Wood, Kevin Kupcho, Keith V. Wood, James Robert Hartnett, Sergiy Levin, Thomas A. Kirkland, Danette L. Daniels, Carolyn C. Woodroofe, Andrew L. Niles, Melanie Dart, Kristopher Zimmerman, Thomas Machleidt, Chad Zimprich, Rachel Friedman Ohana, Yi-Qiang Cheng, Michael R. Slater
Publikováno v:
Nature Communications
The therapeutic action of drugs is predicated on their physical engagement with cellular targets. Here we describe a broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16129acbaa5af847dd39d9c7736dbc2f
http://europepmc.org/articles/PMC4686764
http://europepmc.org/articles/PMC4686764
NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells
Autor:
Marie K. Schwinn, Thomas H. Lubben, Lance P. Encell, Thomas Machleidt, Christopher T. Eggers, Thomas A. Kirkland, Mary P. Hall, Brock Binkowski, Keith V. Wood, Braeden L. Butler, Paul Otto, Kris Zimmerman, Andrew S. Dixon, Monika G. Wood
Publikováno v:
ACS chemical biology. 11(2)
Protein-fragment complementation assays (PCAs) are widely used for investigating protein interactions. However, the fragments used are structurally compromised and have not been optimized nor thoroughly characterized for accurately assessing these in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d7c531afccfd9736db86d62401f90fa
https://pubmed.ncbi.nlm.nih.gov/26569370
https://pubmed.ncbi.nlm.nih.gov/26569370