Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Monica Schold"'
Publikováno v:
DNA. 8:135-142
Using oligonucleotide hybridization, single and multiple nucleotide differences between alleles were detected directly in genomic DNA without electrophoretic separation. The DNA was immobilized in depressions in an agarose gel (in situ dots) and hybr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0a568c6275f107f67c69a2cfa099204
https://doi.org/10.1089/dna.1.1989.8.135
https://doi.org/10.1089/dna.1.1989.8.135
Publikováno v:
DNA. 3:469-477
Oligonucleotide-directed mutagenesis using plasmid vectors has been simplified by introducing two changes to the previous method. First, template preparation has been simplified by using the covalently closed circular plasmid DNA directly for mutagen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a9a7dc9e49b9bf8eeba43c3c23d49cc
https://doi.org/10.1089/dna.1.1984.3.469
https://doi.org/10.1089/dna.1.1984.3.469
Publikováno v:
Immunogenetics. 16:1-9
A mouse cDNA library derived from the EL4 cell line (b-haplotype) was screened with a probe containing a small part of the H-2Kb coding region. One of the clones isolated, pH203, encodes a protein whose deduced amino acid sequence is identical with t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62f835853e5d3f1d353d512ab08507d3
https://doi.org/10.1007/bf00364437
https://doi.org/10.1007/bf00364437
Publikováno v:
Proceedings of the National Academy of Sciences. 79:3270-3274
A library of cloned cDNAs constructed from the poly(A)+RNA of the murine thymoma cell line EL4 (b haplotype) was screened with a probe encoding a short region of the H-2Kb transplantation antigen. One of the clones isolated, pH202, contains a region
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9fc43682622fea1f5bbd40467028d63
https://doi.org/10.1073/pnas.79.10.3270
https://doi.org/10.1073/pnas.79.10.3270
Autor:
R B Wallace, C Morin, Hirataka Ito, Merrie Jo Johnson, Monica Schold, Keiichi Itakura, Ike Y, Reyes Aa
Publikováno v:
Immunogenetics. 14:383-392
Based on the published amino-acid sequence of H-2Kb, we synthesized a mixture of eight 16-base long oligodeoxyribonucleotides representing all possible coding sequences for residues 51-56 (Trp-Met-Glu-Gln-Glu-Gly). The hexadecanucleotide mixture was
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f85f444cfadf917ddc96418ef13688e4
https://doi.org/10.1007/bf00373318
https://doi.org/10.1007/bf00373318
Publikováno v:
Nucleic acids research. 9(15)
A nonadecanucleotide has been used both as a site specific mutagen to introduce a T leads to A transversion mutation in the human beta-globin gene cloned in pBR322 as well as a probe to screen transformed colonies for the desired mutant. The specific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::876acf43333fb96ca219112dba8bb37c
https://pubmed.ncbi.nlm.nih.gov/7279669
https://pubmed.ncbi.nlm.nih.gov/7279669
Publikováno v:
Science (New York, N.Y.). 209(4463)
Many eukaryotic genes contain intevening sequences, segments of DNA that interrupt the continuity of the gene. They are removed from RNA transcripts of the gene by a process known as splicing. The intervening sequence in a yeast tyrosine transfer RNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::924d62d5cbd6b1f557eba2ed5f1ee9e8
https://pubmed.ncbi.nlm.nih.gov/6997991
https://pubmed.ncbi.nlm.nih.gov/6997991
Publikováno v:
Gene. 20(3)
The yeast tRNATyr ochre suppressor SUP6 gene and a derivative of this gene in which the 14-bp intervening sequence has been deleted, SUP6 delta, have been examined for functional expression in Escherichia coli. The SUP6 delta, but not the SUP6, gene
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d797d1c21363cd75447872d2cc27df56
https://pubmed.ncbi.nlm.nih.gov/6819979
https://pubmed.ncbi.nlm.nih.gov/6819979
Publikováno v:
DNA (Mary Ann Liebert, Inc.). 6(5)
A plasmid DNA vector is described that is suitable for cloning synthetic DNA sequences. These cloned synthetic DNA sequences can be transcribed in vitro to produce oligoribonucleotides of defined sequence. Transcription is directed by a promoter base
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::748919494d4c62dbc3da840f841d3860
https://pubmed.ncbi.nlm.nih.gov/3677997
https://pubmed.ncbi.nlm.nih.gov/3677997