High-Throughput 16S rRNA Sequencing to Assess Potentially Active Bacteria and Foodborne Pathogens: A Case Example in Ready-to-Eat Food

Autor: Marina Mira Miralles, Lucia Maestre-Carballa, Monica Lluesma-Gomez, Manuel Martinez-Garcia

Publikováno v: Foods, Vol 8, Iss 10, p 480 (2019)

Technologies to detect the entire bacterial diversity spectra and foodborne pathogens in food represent a fundamental advantage in the control of foodborne illness. Here, we applied high-throughput 16S rRNA sequencing of amplicons obtained by PCR and

Externí odkaz: https://doaj.org/article/e10b67ef237043a69f7966396ba8620c

Benchmarking of s ingle‐virus genomics: a new tool for uncovering the virosphere

Autor: Monica Lluesma Gomez, Oscar Fornas, Ananda S. Bhattacharjee, Manuel Martinez-Garcia, Inmaculada Garcia-Heredia, Joaquín Martínez Martínez

Publikováno v: RUA. Repositorio Institucional de la Universidad de Alicante
Universidad de Alicante (UA)

Metagenomics and single‐cell genomics have enabled the discovery of relevant uncultured microbes. Recently, single‐virus genomics (SVG), although still in an incipient stage, has opened new avenues in viral ecology by allowing the sequencing of o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b81f6fcb98b00b1e062b15d534a507b
https://doi.org/10.1111/1462-2920.15375

Droplet Digital PCR for Estimating Absolute Abundances of Widespread Pelagibacter Viruses

Autor: Monica Lluesma Gomez, Inmaculada Garcia-Heredia, Joaquín Martínez Martínez, Lucia Maestre-Carballa, Francisco Martinez-Hernandez, Manuel Martinez-Garcia

Publikováno v: Frontiers in Microbiology
Frontiers in Microbiology, Vol 10 (2019)
RUA. Repositorio Institucional de la Universidad de Alicante
Universidad de Alicante (UA)

Absolute abundances of prokaryotes are typically determined by FISH. Due to the lack of a universal conserved gene among all viruses, metagenomic fragment recruitment is commonly used to estimate the relative viral abundance. However, the paucity of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a6cc5e101255a580505e2738e631769
https://hdl.handle.net/10045/92869

Single-cell genomics uncover Pelagibacter as the putative host of the extremely abundant uncultured 37-F6 viral population in the ocean

Autor: Francisco Martinez-Hernandez, Monica Lluesma Gomez, Francisco Rodriguez-Valera, Inmaculada Garcia-Heredia, Jose M. Haro-Moreno, Mario López-Pérez, Manuel Martinez-Garcia, Lucia Maestre-Carballa, Oscar Fornas

Publikováno v: The ISME journal. 13(1)

The identification of relevant virus–host pairs that globally account for a large pool of carbon and nutrients in the ocean is paramount to build accurate ecological models. A previous work using single-virus genomics led to the discovery of the un

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a350dc00736bd24c834f6f61b9b7a3d5
https://pubmed.ncbi.nlm.nih.gov/30228380

Single-virus genomics reveals hidden cosmopolitan and abundant viruses

Autor: Francisco Martinez-Hernandez, Oscar Fornas, Maria José de la Cruz Peña, Monica Lluesma Gomez, Josep M. Gasol, Manuel Martinez-Garcia, Benjamin Bolduc, Silvia G. Acinas, Josefa Antón, Joaquín Martínez Martínez, Francisco Rodriguez-Valera, Matthew B. Sullivan, Riccardo Rosselli

Publikováno v: REDIUMH. Depósito Digital de la UMH
instname
Nature Communications
RUA. Repositorio Institucional de la Universidad de Alicante
Universidad de Alicante (UA)
Recercat. Dipósit de la Recerca de Catalunya
Nature Communications, Vol 8, Iss 1, Pp 1-13 (2017)
Digital.CSIC. Repositorio Institucional del CSIC

Martínez-Hernández, Francisco J. et al.-- 13 pages, 6 figures, 1 table, data availability, supplementary information https://dx.doi.org/10.1038/ncomms15892
Microbes drive ecosystems under constraints imposed by viruses. However, a lack of viru

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ae067d272b4c25aa45b4dc94ba8627a
http://hdl.handle.net/11000/4836