Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary.

Autor: Rihab Ameri, Elisabeth Laville, Gabrielle Potocki-Véronèse, Sahar Trabelsi, Monia Mezghani, Fatma Elgharbi, Samir Bejar

Publikováno v: PLoS ONE, Vol 13, Iss 3, p e0194621 (2018)

Dromedaries are capable of digesting plant cell wall with high content of lignocellulose of poor digestibility. Consequently, their intestinal microbiota can be a source of novel carbohydrate-active enzymes (CAZymes). To the best of our knowledge, no

Externí odkaz: https://doaj.org/article/6a382b3359e94b8fbb62e4d146284cd4

A thermophilic and thermostable xylanase from Caldicoprobacter algeriensis: Recombinant expression, characterization and application in paper biobleaching

Autor: Sonia Jemli, Samir Bejar, Sawssan Neifar, Bassem Jaouadi, Monia Mezghani, Sonia Mhiri, Amel Bouanane-Darenfed, Rihab Ameri, Khelifa Bouacem

Publikováno v: International Journal of Biological Macromolecules. 164:808-817

A novel xylanase gene xynBCA, encoding a polypeptide of 439 residues (XynBCA), was cloned from Caldicoprobacter algeriensis genome and recombinantly expressed in Escherichia coli BL21(DE3). The amino acid sequence analysis showed that XynBCA belongs

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bcb9856a3b58ecb6a78bb6e8f6d115e
https://doi.org/10.1016/j.ijbiomac.2020.07.162

Aspergillus oryzae S2 AmyA amylase expression in Pichia pastoris: production, purification and novel properties

Autor: Aïda Hmida-Sayari, Sahar Trabelsi, Sameh Ben Mabrouk, Rihab Ameri, Samir Bejar, Monia Mezghani, Mouna Sahnoun, Fatma Elgharbi

Publikováno v: Molecular biology reports. 46(1)

A synthetic cDNA-AmyA gene was cloned and successfully expressed in Pichia pastoris as a His-tagged enzyme under the methanol inducible AOX1 promoter. High level of extracellular amylase production of 72 U/mL was obtained after a 72 h induction by me

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f16b6edb460f680955ba870590e2ddb
https://pubmed.ncbi.nlm.nih.gov/30535895

Characterization of glucansucrase and dextran from Weissella sp. TN610 with potential as safe food additives

Autor: Sandrine Morel, Wacim Bejar, Hichem Chouayekh, Catherine Fontagné-Faucher, Myriam Amari, Samir Bejar, Monia Mezghani, Valérie Gabriel, Emmanuelle Maguin

Publikováno v: International Journal of Biological Macromolecules
International Journal of Biological Macromolecules, Elsevier, 2013, 52, pp.125-132. ⟨10.1016/j.ijbiomac.2012.09.014⟩
International Journal of Biological Macromolecules, 2013, 52, pp.125-132. ⟨10.1016/j.ijbiomac.2012.09.014⟩

Pear-derived Weissella sp. TN610 produced extracellular glycosyltransferase activity responsible for the synthesis of soluble exopolysaccharide from sucrose. Acid and dextranase-catalyzed hydrolysis revealed that the synthesized polymer was a glucan.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff8d687634d1cb85c911f563f71d403e
https://doi.org/10.1016/j.ijbiomac.2012.09.014

Glucose isomerase of the Streptomyces sp. SK strain: purification, sequence analysis and implication of alanine 103 residue in the enzyme thermostability and acidotolerance

Autor: Samir Bejar, Mohamed Ali Borgi, Richard Haser, Karima Srih-Belguith, Monia Mezghani, Mamdouh Ben Ali, Samuel Tranier

Publikováno v: Biochimie. 86:561-568

The glucose isomerase gene (xylA) from the Streptomyces sp. SK strain encodes a 386-amino-acid protein (42.7 kDa) showing extensive identities with many other bacterial glucose isomerases. We have shown by gel filtration chromatography and SDS-PAGE a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::179b79f0e2aaabcd937552ff05fb89fd
https://doi.org/10.1016/j.biochi.2004.07.003