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Akademický článek

Knockout of Arabidopsis thaliana VEP1, Encoding a PRISE (Progesterone 5β-Reductase/Iridoid Synthase-Like Enzyme), Leads to Metabolic Changes in Response to Exogenous Methyl Vinyl Ketone (MVK)

Autor: Jan Klein, Mona Ernst, Alexander Christmann, Marina Tropper, Tim Leykauf, Wolfgang Kreis, Jennifer Munkert

Publikováno v: Metabolites, Vol 12, Iss 1, p 11 (2021)

Small or specialized natural products (SNAPs) produced by plants vary greatly in structure and function, leading to selective advantages during evolution. With a limited number of genes available, a high promiscuity of the enzymes involved allows the

Externí odkaz: https://doaj.org/article/9290f634e1f04b8dae0ff62e05da2fb4

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Overexpression and RNAi-mediated Knockdown of Two 3β-hydroxy-Δ5-steroid dehydrogenase Genes in Digitalis lanata Shoot Cultures Reveal Their Role in Cardenolide Biosynthesis

Autor: Tim Leykauf, Jan Klein, Mona Ernst, Maja Dorfner, Anastasiia Ignatova, Wolfgang Kreis, Harald Lanig, Jennifer Munkert

Publikováno v: Planta Medica.

3β-hydroxy-Δ5-steroid dehydrogenases (3βHSDs) are supposed to be involved in 5β-cardenolide biosynthesis. Here, a novel 3βHSD (Dl3βHSD2) was isolated from Digitalis lanata shoot cultures and expressed in E. coli. Recombinant Dl3βHSD1 and Dl3β

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cc7f2d3213a6cef04f8d43daaa138941
https://doi.org/10.1055/a-2074-9186

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RNAi-mediated gene knockdown of progesterone 5β-reductases in Digitalis lanata reduces 5β-cardenolide content

Autor: Maja Dorfner, Elisa Horn, Tamara Leupold, Wolfgang Kreis, Tim Leykauf, Jennifer Munkert, Laura Wolf, Jan Klein, Mona Ernst, Anastasiia Ignatova

Publikováno v: Plant Cell Reports

Key message Studying RNAi-mediated DlP5βR1 and DlP5βR2 knockdown shoot culture lines of Digitalis lanata, we here provide direct evidence for the participation of PRISEs (progesterone 5β-reductase/iridoid synthase-like enzymes) in 5β-cardenolide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eda989d3c5345f229b49323fd81f90e4
https://opus4.kobv.de/opus4-fau/frontdoor/index/index/docId/22086

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RNAi mediated knock down of 3β-hydroxysteroid dehydrogenases in Digitalis lanata shoot cultures

Autor: Wolfgang Kreis, T Leykauf, Mona Ernst, J Klein, Jennifer Munkert

Publikováno v: 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::dee68ff7c3f7e2b8c4bb0c1ee8d1edc0
https://doi.org/10.1055/s-0039-3399800

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Expression of3β‐HSDandP5βR, Genes Respectively Coding for Δ5-3β-Hydroxysteroid Dehydrogenase and Progesterone5β‐Reductase, in Leaves and Cell Cultures ofDigitalis lanataEHRH

Autor: Wolfgang Kreis, Frieder Müller-Uri, Vanessa Herl, Mona Ernst, Rodrigo Maia de Pádua

Publikováno v: Planta Medica. 76:923-927

Plants of the genus Digitalis produce 5 beta-cardenolides that are used in the therapy of cardiac insufficiency in humans. 3 beta-Hydroxysteroid dehydrogenase (3 beta-HSD) and progesterone 5 beta-reductase (P5 betaR) are both supposed to be important

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6f0ae658059044103d17300f70338701
https://doi.org/10.1055/s-0030-1250007

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Steroid 5β-reductase from leaves of Vitis vinifera: molecular cloning, expression and modelling

Autor: Mona Ernst, Jennifer Munkert, Frieder Müller-Uri, Mickael Malnoy, Stefan Martens, Manuela Campa

A steroid 5β-reductase gene corresponding to the hypothetical protein LOC100247199 from leaves of Vitis vinifera (var. 'Chardonnay') was cloned and overexpressed in Escherichia coli. The recombinant protein showed 5β-reductase activity when progest

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc53b7a977bcaa2138f35955be194799
http://hdl.handle.net/10449/25732

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Identification and stress-induced expression of three 3β-hydroxysteroid dehydrogenases from Erysimum crepidifolium Rchb. and their putative role in cardenolide biosynthesis

Autor: Wolfgang Kreis, Frieder Müller-Uri, Jennifer Munkert, Mona Ernst

Publikováno v: Phytochemistry. 100

3β-Hydroxysteroid dehydrogenases (3βHSD) are supposed to be involved in cardenolide biosynthesis in plants. Erysimum crepidifolium Rchb., a member of the Brassicaceae accumulating cardenolides, is a close relative to Arabidopsis thaliana. Full leng

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcfad05baf33ae142fe5999c82c8724e
https://pubmed.ncbi.nlm.nih.gov/24512841

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Expression of 3beta-HSD and P5betaR, genes respectively coding for Delta5-3beta-hydroxysteroid dehydrogenase and progesterone 5beta-reductase, in leaves and cell cultures of Digitalis lanata EHRH

Autor: Mona, Ernst, Rodrigo Maia, de Padua, Vanessa, Herl, Frieder, Müller-Uri, Wolfgang, Kreis

Publikováno v: Planta medica. 76(9)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8a58ab2166b23ec2d48959ebcb1f464d
https://pubmed.ncbi.nlm.nih.gov/20514608

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