Design and Validation of Site-Specifically Labeled Single-Domain Antibody-Based Tracers for in Vivo Fluorescence Imaging and Image-Guided Surgery

Autor: Noemi Declerck, Lukasz Mateusiak, Sophie Hernot

Publikováno v: Methods in Molecular Biology ISBN: 9781071620748

Near-infrared fluorescence molecular imaging has become an established preclinical technique to investigate molecular processes in vivo and to study novel therapies. Furthermore, fluorescence molecular imaging is gaining significant interest from cli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22c43d4b9089ff59fb613575eeb7d2cd
https://doi.org/10.1007/978-1-0716-2075-5_20

Real-time imaging of multivesicular body-plasma membrane fusion to quantify exosome release from single cells

Autor: Stephan Huveneers, Guillaume van Niel, Philippe Bun, Frederik J. Verweij, Maarten P. Bebelman, D. Michiel Pegtel

Publikováno v: Nature Protocols, 15(1), 102-121. Nature Publishing Group
Nature Protocols
Nature Protocols, Nature Publishing Group, 2019, Epub ahead of print. ⟨10.1038/s41596-019-0245-4⟩
Bebelman, M P, Bun, P, Huveneers, S, van Niel, G, Pegtel, D M & Verweij, F J 2020, ' Real-time imaging of multivesicular body–plasma membrane fusion to quantify exosome release from single cells ', Nature Protocols, vol. 15, no. 1, pp. 102-121 . https://doi.org/10.1038/s41596-019-0245-4
Nature Protocols, Nature Publishing Group, 2020, 15 (1), pp.102-121. ⟨10.1038/s41596-019-0245-4⟩
Nature protocols, 15(1), 102
Bebelman, M P, Bun, P, Huveneers, S, van Niel, G, Pegtel, D M & Verweij, F J 2020, ' Real-time imaging of multivesicular body-plasma membrane fusion to quantify exosome release from single cells ', Nature Protocols, vol. 15, no. 1, pp. 102-121 . https://doi.org/10.1038/s41596-019-0245-4
Nature protocols, 15(1). Nature Publishing Group

International audience; Exosomes are small extracellular vesicles with a diameter of 40-150 nm, and are implicated in cellular homeostasis and cell-cell communication. They can be secreted in bulk in response to cell-extrinsic and cell-intrinsic sign

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2232114ede5181409d35d5af188fbcd
https://research.vumc.nl/en/publications/2e5bf9a8-20a0-44a9-9f59-ffc37be33e7e

Mitochondrial Dynamics and Activity in Legionella-Infected Cells

Autor: Carmen Buchrieser, Ok-Ryul Song, Pedro Escoll, Priscille Brodin

Publikováno v: Legionella: Methods and Protocols
Carmen Buchrieser; Hubert Hilbi. Legionella: Methods and Protocols, 1921 (13), Springer Science; Business Media, LLC, pp.205-220, 2019, Methods in Molecular Biology (MIMB), 978-1-4939-9047-4 (Print), 978-1-4939-9048-1 (Online). ⟨10.1007/978-1-4939-9048-1_13⟩
Methods in Molecular Biology ISBN: 9781493990474

International audience; The study of Legionella pneumophila interactions with host mitochondria during infection has been historically limited by the techniques available to analyze and quantify mitochondrial dynamics and activity in living cells. Re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be2372f437c9a9003cb049b48b2b5376
https://hal-pasteur.archives-ouvertes.fr/pasteur-02881368

Molecular Recognition Force Spectroscopy for Probing Cell Targeted Nanoparticles In Vitro

Autor: Gomes, CP, Oliveira, H, Ebner, A, Hinterdorfer, P, Pêgo, AP

In the development and design of cell targeted nanoparticle-based systems the density of targeting moieties plays a fundamental role in allowing maximal cell-specific interaction. Here, we describe the use of molecular recognition force spectroscopy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______1406::9cfa450ed17abdc60a1b86d39886d57f
https://hdl.handle.net/10216/126850

Imaging of Human Insulin Secreting Cells with Gd-DOTA-P88, a Paramagnetic Contrast Agent Targeting the Beta Cell Biomarker FXYD2gammaa

Autor: Stéphane Demine, Isabelle Millard, Martin Gotthardt, Alexander Balhuizen, Decio L. Eizirik, Satya Narayana Murthy Chilla, Maïté Fereau, Raphael Scharfmann, Vinciane Debaille, Sophie Laurent, Serge Goldman, Lieke Joosten, Carmen Burtea, Piero Marchetti, Dominique Egrise

Publikováno v: Molecules, 23, 2100
Molecules, Vol 23, Iss 9, p 2100 (2018)
Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Molecules, 23, 9, pp. 2100
Molecules
Volume 23
Issue 9
Molecules, 23 (9

Non-invasive imaging and quantification of human beta cell mass remains a major challenge. We performed pre-clinical in vivo validation of a peptide previously discovered by our group, namely, P88 that targets a beta cell specific biomarker, FXYD2&ga

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7f7bed1e8a7cd99e8d66cc89185b42b
http://hdl.handle.net/2066/196910