Optogenetic Reporters Delivered as mRNA Facilitate Repeatable Action Potential and Calcium Handling Assessment in Human iPSC-Derived Cardiomyocytes.

Autor: Yiangou L; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., Blanch-Asensio A; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., de Korte T; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., Miller DC; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., van Meer BJ; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., Mol MPH; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., van den Brink L; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., Brandão KO; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., Mummery CL; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands., Davis RP; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.

Publikováno v: Stem cells (Dayton, Ohio) [Stem Cells] 2022 Jul 27; Vol. 40 (7), pp. 655-668.

Isogenic Sets of hiPSC-CMs Harboring Distinct KCNH2 Mutations Differ Functionally and in Susceptibility to Drug-Induced Arrhythmias.

Autor: Brandão KO; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., van den Brink L; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., Miller DC; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., Grandela C; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., van Meer BJ; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., Mol MPH; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., de Korte T; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., Tertoolen LGJ; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., Mummery CL; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands., Sala L; Istituto Auxologico Italiano, IRCCS, Laboratory of Cardiovascular Genetics, 20095 Milan, Italy., Verkerk AO; Department of Medical Biology, Amsterdam UMC, University of Amsterdam, 1105AZ Amsterdam, The Netherlands; Department of Experimental Cardiology, Amsterdam UMC, University of Amsterdam, 1105AZ Amsterdam, The Netherlands., Davis RP; Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, The Netherlands. Electronic address: r.p.davis@lumc.nl.

Publikováno v: Stem cell reports [Stem Cell Reports] 2020 Nov 10; Vol. 15 (5), pp. 1127-1139.

MUSCLEMOTION: A Versatile Open Software Tool to Quantify Cardiomyocyte and Cardiac Muscle Contraction In Vitro and In Vivo.

Autor: Sala L; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., van Meer BJ; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Tertoolen LGJ; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Bakkers J; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Bellin M; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Davis RP; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Denning C; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Dieben MAE; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Eschenhagen T; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Giacomelli E; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Grandela C; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Hansen A; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Holman ER; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Jongbloed MRM; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Kamel SM; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Koopman CD; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Lachaud Q; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Mannhardt I; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Mol MPH; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Mosqueira D; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Orlova VV; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Passier R; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Ribeiro MC; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Saleem U; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Smith GL; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Burton FL; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.)., Mummery CL; From the Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands (L.S., B.J.v.M., L.G.J.T., M.B., R.P.D., M.A.E.D., E.G., C.G., M.R.M.J., M.P.H.M., V.V.O., R.P., C.L.M.); Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Science, University of Glasgow, United Kingdom (Q.L., G.L.S., F.L.B.); Hubrecht Institute - Royal Netherlands Academy of Arts and Sciences, Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); Department of Stem Cell Biology, University of Nottingham, University Park, Nottingham, United Kingdom (C.D., D.M.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Germany (T.E., A.H., I.M., U.S.); DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck (T.E., A.H., I.M., U.S.); Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany (U.S.); Hart Long Centrum, Leiden University Medical Center, The Netherlands (E.R.H., M.R.M.J.); Department of Applied Stem Cell Technologies, University of Twente, Enschede, The Netherlands (R.P., M.C.R., C.L.M.).; Division of Heart and Lungs, Department of Medical Physiology, University Medical Center Utrecht, The Netherlands (J.B., S.M.K., C.D.K.); and Clyde Biosciences, Ltd, BioCity Scotland, United Kingdom (G.L.S., F.L.B.). c.l.mummery@lumc.nl.

Publikováno v: Circulation research [Circ Res] 2018 Feb 02; Vol. 122 (3), pp. e5-e16. Date of Electronic Publication: 2017 Dec 27.