Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Mohan S. Saini"'
Autor:
Deirdre R. Meldrum, Mohan S. Saini, Mark R. Holl, Charles H. Fisher, P.N. Ngatchou, D.A. Donaldson, T.T.H. Ren, Jianchun Dong, William H. Pence, David L. Cunningham, Stephen E. Moody
Publikováno v:
IEEE Transactions on Automation Science and Engineering. 3:141-151
This paper describes the development of a real-time polymerase chain reaction testbed for the analysis of up to 48 1- to 2-/spl mu/L reactions in glass capillaries. It is suitable for high-throughput experimentation when used as a downstream module f
Autor:
Shawn McGuire, Mohan S. Saini, T.T.H. Ren, Mark R. Holl, Charles H. Fisher, David L. Cunningham, D.A. Donaldson, Peter Wiktor, D.R. Meldram, William H. Pence, Stephen E. Moody
Publikováno v:
CASE
A capillary-based fluid handling system, the ACAPELLA-5K (ASK), has been developed to prepare precise mixtures of sample and reagents in glass capillaries at a rate of 5000 preparations in 8 hours. Following a user-defined protocol the system archite
Autor:
Mohan S. Saini, Deirdre R. Meldrum, Mark R. Holl, Charles H. Fisher, Stephen E. Moody, M.P. Moore, Peter Wiktor, William H. Pence, David L. Cunningham
Publikováno v:
IROS
A capillary-based fluid handling system bas been developed to process 5000 samples in 8 hours. The system takes deoxyribonucleic acid (DNA) or other chemical samples and automatically processes them as specified in a user-defined protocol with aspira
Autor:
Stephen E. Moody, David L. Cunningham, A. Cookson, M.P. Moore, Mohan S. Saini, Deirdre R. Meldrum, William H. Pence, M. Kidd, Ling Sheng Jang, P.J. Wiktor, Mark R. Holl, Charles H. Fisher
Publikováno v:
2001 IEEE/ASME International Conference on Advanced Intelligent Mechatronics. Proceedings (Cat. No.01TH8556).
The Genomation Laboratory at the University of Washington has developed an automated fluid handling system called "Acapella" to prepare microliter reactions for genome analysis. Reactions such as restriction enzyme digests, polymerase chain reactions
Autor:
Tobias P. Mann, Stephen E. Moody, William H. Pence, Daniel E. Sabath, David L. Cunningham, Mark R. Holl, Charles H. Fisher, Mohan S. Saini, Deirdre R. Meldrum
Publikováno v:
Micro Total Analysis Systems 2001 ISBN: 9789401038935
This paper describes a system for quantification of template DNA in a sample using fluorescence detection of DNA amplification during a real-time PCR reaction in a 5 µl glass capillary channel. The capillary system allows aliquots to be drawn from s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e23b79eace9de4fdda1b33422f1e6681
https://doi.org/10.1007/978-94-010-1015-3_250
https://doi.org/10.1007/978-94-010-1015-3_250
Publikováno v:
Micro Total Analysis Systems 2001 ISBN: 9789401038935
The development of a procedure based on streptavidin magnetic beads for purification of sequencing reaction products in microfluidic glass capillary channels is presented. Recovery of the DNA, up to 80%, was achieved using big dye terminator reagents
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::30fd7bfe70ad3baa9563992168bfe4bc
https://doi.org/10.1007/978-94-010-1015-3_45
https://doi.org/10.1007/978-94-010-1015-3_45
Publikováno v:
Journal of Biological Chemistry. 259:2208-2213
The stereochemical course of the phospho transfer catalyzed by homogeneous human prostatic acid phosphatase was investigated using 31P nuclear magnetic resonance spectroscopy. Transphosphorylation from phenyl-(R)-[15O, 17O, 18O]phosphate to (S)-propa
Publikováno v:
Phytochemistry. 15:1497-1500
The particle-bound enzyme catalysing the conversion of dl -4-γ,γ-diinethylallyltryptophan into clavicipitic acid in Claviceps purpurea PRL 1980 has been solubilised. The K m value and pH optimum were determined and the substrate specfficity of the
Autor:
Mohan S. Saini, Robert L. Van Etten
Publikováno v:
The Prostate. 2:359-368
We describe an assay method using choline O-phosphate as a substrate for the measurement of serum prostatic acid phosphatase as an aid in the diagnosis of prostatic cancer. Choline phosphate is hydrolyzed by homogeneous prostatic acid phosphatase, an
Autor:
Mohan S. Saini, Robert L. Van Etten
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Enzymology. 526:468-478
Two isoenzymes of rat liver acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum) EC 3.1.3.2) have been purified to homogeneity, at least one of these for the first time. Both of the rat liver isoenzymes have identical specific