Zobrazeno 1 - 10
of 63
pro vyhledávání: '"Mohammad M. Ataai"'
Publikováno v:
Biotechnology Journal. 7:449-460
Pyruvate kinase-deficient Escherichia coli (PB25) is a low by-product-producing yet fast-growing mutant that has been shown to have technological potential. Determining the flux limits through finding the extreme point flux sets was previously report
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::070749370d3f730267f82c525e621561
https://doi.org/10.1002/biot.201100338
https://doi.org/10.1002/biot.201100338
Publikováno v:
Biotechnology Progress. 28:137-145
Escherichia coli is a favored host for rapid, scalable expression of recombinant proteins for academic, commercial, or therapeutic use. To maximize its economic advantages, however, it must be coupled with robust downstream processes. Affinity chroma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fee07d0420e9b7065eee8633ac50961d
https://doi.org/10.1002/btpr.703
https://doi.org/10.1002/btpr.703
Autor:
John J. Cardamone, Robert R. Beitle, McKinzie Fruchtl, Tamanna Sultana, Mohammad M. Ataai, Patrick Bartlow, Guy Uechi
Publikováno v:
Protein Expression and Purification. 78:216-224
Immobilized metal affinity chromatography (IMAC) is a widely used purification tool for the production of active, soluble recombinant proteins. Escherichia coli proteins that routinely contaminate IMAC purifications have been characterized to date. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e82567671c772b50020e7c99b9386c7b
https://doi.org/10.1016/j.pep.2011.04.021
https://doi.org/10.1016/j.pep.2011.04.021
Autor:
Mohammad M. Ataai, Robert Dilmore, Yee Soong, Sheila W. Hedges, Richard R. Koepsel, Zhu Liu, Craig Griffith
Publikováno v:
International Journal of Greenhouse Gas Control. 3:401-410
A novel CO 2 separation concept is described wherein the enzyme carbonic anhydrase (CA) is used to increase the overall rate of CO 2 absorption after which hydrated CO 2 reacts with regenerable amine-bearing polyacrylamide buffering beads (PABB). Fol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7dfe0fe3c6f0079b42d82deba62064bc
https://doi.org/10.1016/j.ijggc.2009.01.004
https://doi.org/10.1016/j.ijggc.2009.01.004
Autor:
Richard R. Koepsel, Nathan Domagalski, Zhu Liu, Michael M. Domach, Drew S. Cunningham, Mohammad M. Ataai
Publikováno v:
Journal of Bacteriology. 191:3041-3049
Previously established consequences of abolishing pyruvate kinase (Pyk) activity in Escherichia coli during aerobic growth on glucose include reduced acetate production, elevated hexose monophosphate (HMP) pathway flux, elevated phosphoenolpyruvate c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3efa7cbee7290cb14c637ae0c5067d3c
https://doi.org/10.1128/jb.01422-08
https://doi.org/10.1128/jb.01422-08
Autor:
Robert R. Beitle, Mohammad M. Ataai, Rajaramesh Varakala, Patrick Bartlow, Richard R. Koepsel, Zhu Liu
Publikováno v:
Journal of Chromatography A. 1216:2433-2438
After some initial optimization, a downstream process comprised of one or several chromatography steps removes the majority of the host proteins and achieves a reasonable degree of purification. The separation of remaining contaminant proteins from t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1be4a1ff61cd7a09833e1c1d64ce3582
https://doi.org/10.1016/j.chroma.2009.01.020
https://doi.org/10.1016/j.chroma.2009.01.020
Autor:
Ram N. Trivedi, Parvez Akhtar, Saleem A. Khan, Jonathan Meade, Mohammad M. Ataai, Michael M. Domach, Patrick Bartlow
Publikováno v:
Microbial Cell Factories
When the replication of a plasmid based on sucrose selection is deregulated via the inc1 and inc2 mutations, high copy numbers (7,000 or greater) are attained while the growth rate on minimal medium is negligibly affected. Adaptions were assumed to b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::890abd27b36d36dde7a5e3bad83b60c3
https://doi.org/10.1186/s12934-015-0212-x
https://doi.org/10.1186/s12934-015-0212-x
Publikováno v:
Metabolic Engineering. 8:491-507
We have developed a sequential set of computational screens that may prove useful for evaluating analyte sets for their ability to accurately report on metabolic fluxes. The methodology is problem-centric in that the screens are used in the context o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a24c7fb7d822ca78035c86b419c5d9d
https://doi.org/10.1016/j.ymben.2006.05.001
https://doi.org/10.1016/j.ymben.2006.05.001
Autor:
Patrick Bartlow, Michael M. Domach, Ram N. Trivedi, Parvez Akhtar, Saleem A. Khan, Mohammad M. Ataai, Jonathan Meade
For small-copy-number pUC-type plasmids, the inc1 and inc2 mutations, which deregulate replication, were previously found to increase the plasmid copy number 6- to 7-fold. Because plasmids can exert a growth burden, it was not clear if further amplif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::037c101f490f9484e1183fadf0c391e7
https://europepmc.org/articles/PMC4249171/
https://europepmc.org/articles/PMC4249171/
Publikováno v:
Applied and Environmental Microbiology. 71:7122-7129
We investigated whether the yield of the B vitamin folic acid could be elevated in Bacillus subtilis . Strategies for increasing the folic acid yield were investigated by employing computer-aided flux analysis and mutation. Controlling the activity o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba68f32989906a0c121c690a65d01b4a
https://doi.org/10.1128/aem.71.11.7122-7129.2005
https://doi.org/10.1128/aem.71.11.7122-7129.2005