Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Modesto Redrejo-Rodriguez"'
Autor:
Dario Cilleros-Rodriguez, Raquel Martin-Morales, Pablo Barbeito, Abhijit Deb Roy, Abdelhalim Loukil, Belen Sierra-Rodero, Gonzalo Herranz, Olatz Pampliega, Modesto Redrejo-Rodriguez, Sarah C Goetz, Manuel Izquierdo, Takanari Inoue, Francesc R Garcia-Gonzalo
Publikováno v:
eLife, Vol 11 (2022)
Primary cilia are sensory membrane protrusions whose dysfunction causes ciliopathies. INPP5E is a ciliary phosphoinositide phosphatase mutated in ciliopathies like Joubert syndrome. INPP5E regulates numerous ciliary functions, but how it accumulates
Externí odkaz:
https://doaj.org/article/cfd0cf2219bf4640ab9dff7407cfa7b3
Publikováno v:
Bio-Protocol, Vol 8, Iss 1 (2018)
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are immunoprecipitated in HA-a
Externí odkaz:
https://doaj.org/article/0ee0fb4d367040c6942e0ea165d6017d
Publikováno v:
Frontiers in Molecular Biosciences, Vol 3 (2016)
Bacillus subtilis phage Φ29 has a linear, double-stranded DNA 19 kb long with an inverted terminal repeat of 6 nucleotides and a protein covalently linked to the 5’ ends of the DNA. This protein, called terminal protein (TP), is the primer for the
Externí odkaz:
https://doaj.org/article/d7d534c31eac4e088cf463ec09bf8954
Publikováno v:
Bio-Protocol, Vol 4, Iss 6 (2014)
This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. In the orig
Externí odkaz:
https://doaj.org/article/d5ce1453aa1549a3b0dfa1598a2731ee
Autor:
Raquel Martin-Morales, Dario Cilleros-Rodriguez, Pablo Barbeito, Abhijit Deb Roy, Abdelhalim Loukil, Belen Sierra-Rodero, Gonzalo Herranz, Olatz Pampliega, Modesto Redrejo-Rodriguez, Sarah C Goetz, Manuel Izquierdo, Takanari Inoue, Francesc R Garcia-Gonzalo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9bdaed5d7ecb87a0d0ea0bf4e31598c1
https://doi.org/10.7554/elife.78383.sa2
https://doi.org/10.7554/elife.78383.sa2
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 11, p 9331 (2023)
In the same way that specialized DNA polymerases (DNAPs) replicate cellular and viral genomes, only a handful of dedicated proteins from various natural origins as well as engineered versions are appropriate for competent exponential amplification of
Externí odkaz:
https://doaj.org/article/44decf32ac6e415cb353316d9525bec8
Autor:
Ana Lechuga, Cédric Lood, Margarita Salas, Vera van Noort, Rob Lavigne, Modesto Redrejo-Rodríguez
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 10, Iss 9, Pp 2927-2939 (2020)
Bacillus thuringiensis is the most used biopesticide in agriculture. Its entomopathogenic capacity stems from the possession of plasmid-borne insecticidal crystal genes (cry), traditionally used as discriminant taxonomic feature for that species. As
Externí odkaz:
https://doaj.org/article/c0e1f6f511e7428fbfd3c11b380fe4c5
Publikováno v:
Frontiers in Microbiology, Vol 12 (2021)
Bam35 and related betatectiviruses are tail-less bacteriophages that prey on members of the Bacillus cereus group. These temperate viruses replicate their linear genome by a protein-primed mechanism. In this work, we have identified and characterized
Externí odkaz:
https://doaj.org/article/678ceba157ed48efb249715cd8d82af9
Autor:
Ana Lechuga, Cédric Lood, Mónica Berjón-Otero, Alicia del Prado, Jeroen Wagemans, Vera van Noort, Rob Lavigne, Margarita Salas, Modesto Redrejo-Rodríguez
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 20, p 11105 (2021)
Bacillus virus Bam35 is the model Betatectivirus and member of the family Tectiviridae, which is composed of tailless, icosahedral, and membrane-containing bacteriophages. Interest in these viruses has greatly increased in recent years as they are th
Externí odkaz:
https://doaj.org/article/3c297254e8994fb791d46bbfa36a6274
Autor:
Modesto Redrejo-Rodríguez, Carlos D. Ordóñez, Mónica Berjón-Otero, Juan Moreno-González, Cristian Aparicio-Maldonado, Patrick Forterre, Margarita Salas, Mart Krupovic
Publikováno v:
Cell Reports, Vol 21, Iss 6, Pp 1574-1587 (2017)
Summary: Family B DNA polymerases (PolBs) play a central role during replication of viral and cellular chromosomes. Here, we report the discovery of a third major group of PolBs, which we denote primer-independent PolB (piPolB), that might be a link
Externí odkaz:
https://doaj.org/article/41f495adcdde458ab33b5a430e23ff58