Heterochronous multiplex real-time PCR with intercalating dye using uracil-DNA N-glycosylase (UNG) and multiple primer pairs to revaluate post PCR product.

Autor: Mizumoto-Teramura Y; Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan., Kamogashira T; Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan., Kondo K; Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan., Yamasoba T; Department of Otolaryngology, Tokyo Teishin Hospital, Japan.

Publikováno v: MethodsX [MethodsX] 2024 Jun 22; Vol. 13, pp. 102818. Date of Electronic Publication: 2024 Jun 22 (Print Publication: 2024).