Tetraspanin immunocapture phenotypes extracellular vesicles according to biofluid source but may limit identification of multiplexed cancer biomarkers

Autor: Koster Hj, Swindell Hs, Gary S. Leiserowitz, Tatu Rojalin, Aijun Wang, Randy P. Carney, Brostoff T, Mizenko Rr

Tetraspanin expression of extracellular vesicles (EVs) is often used as a surrogate for their general detection and classification from background contaminants. This common practice typically assumes a consistent expression of tetraspanins across EV

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6b19e84eb677f8760bce4f21aafd66cb
https://doi.org/10.1101/2021.03.02.433595

Harnessing extracellular vesicle heterogeneity for diagnostic and therapeutic applications.

Autor: Carney RP; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA. rcarney@ucdavis.edu., Mizenko RR; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA., Bozkurt BT; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA., Lowe N; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA., Henson T; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA.; Center for Surgical Bioengineering, Department of Surgery, University of California, Davis, School of Medicine, Sacramento, CA, USA., Arizzi A; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA., Wang A; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA.; Center for Surgical Bioengineering, Department of Surgery, University of California, Davis, School of Medicine, Sacramento, CA, USA., Tan C; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA., George SC; Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA. scgeorge@ucdavis.edu.; Center for Surgical Bioengineering, Department of Surgery, University of California, Davis, School of Medicine, Sacramento, CA, USA. scgeorge@ucdavis.edu.

Publikováno v: Nature nanotechnology [Nat Nanotechnol] 2024 Oct 28. Date of Electronic Publication: 2024 Oct 28.

Orthogonal analysis reveals inconsistencies in cargo loading of extracellular vesicles.

Autor: Lowe NM; Department of Biomedical Engineering University of California Davis California USA., Mizenko RR; Department of Biomedical Engineering University of California Davis California USA., Nguyen BB; Department of Biomedical Engineering University of California Davis California USA., Chiu KL; Department of Biomedical Engineering University of California Davis California USA., Arun V; Department of Biomedical Engineering University of California Davis California USA., Panitch A; Department of Biomedical Engineering University of California Davis California USA.; Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology and Emory University Atlanta Georgia USA., Carney RP; Department of Biomedical Engineering University of California Davis California USA.

Publikováno v: Journal of extracellular biology [J Extracell Biol] 2024 Aug 23; Vol. 3 (8), pp. e70003. Date of Electronic Publication: 2024 Aug 23 (Print Publication: 2024).

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.

Autor: Welsh JA; Translational Nanobiology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA., Goberdhan DCI; Nuffield Department of Women's and Reproductive Health, University of Oxford, Women's Centre, John Radcliffe Hospital, Oxford, UK., O'Driscoll L; School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin, Ireland.; Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland.; Trinity St. James's Cancer Institute, Trinity College Dublin, Dublin, Ireland., Buzas EI; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.; HCEMM-SU Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary.; HUN-REN-SU Translational Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary., Blenkiron C; Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand., Bussolati B; Department of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy., Cai H; University of Georgia, Athens, Georgia, USA., Di Vizio D; Department of Surgery, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, California, USA., Driedonks TAP; Department CDL Research, University Medical Center Utrecht, Utrecht, The Netherlands., Erdbrügger U; University of Virginia Health System, Charlottesville, Virginia, USA., Falcon-Perez JM; Exosomes Laboratory, Center for Cooperative Research in Biosciences, Basque Research and Technology Alliance, Derio, Spain.; Metabolomics Platform, Center for Cooperative Research in Biosciences, Basque Research and Technology Alliance, Derio, Spain.; IKERBASQUE, Basque Foundation for Science, Bilbao, Spain., Fu QL; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.; Extracellular Vesicle Research and Clinical Translational Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China., Hill AF; Institute for Health and Sport, Victoria University, Melbourne, Australia., Lenassi M; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia., Lim SK; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.; Paracrine Therapeutics Pte. Ltd., Singapore, Singapore.; Department of Surgery, YLL School of Medicine, National University Singapore, Singapore, Singapore., Mahoney MG; Thomas Jefferson University, Philadelphia, Pennsylvania, USA., Mohanty S; Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India., Möller A; Chinese University of Hong Kong, Hong Kong, Hong Kong S.A.R.; QIMR Berghofer Medical Research Institute, Brisbane, Australia., Nieuwland R; Laboratory of Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands.; Amsterdam Vesicle Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands., Ochiya T; Tokyo Medical University, Tokyo, Japan., Sahoo S; Icahn School of Medicine at Mount Sinai, New York, New York, USA., Torrecilhas AC; Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários, Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo (UNIFESP) Campus Diadema, Diadema, Brazil., Zheng L; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China., Zijlstra A; Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.; Genentech, South San Francisco, California, USA., Abuelreich S; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA., Bagabas R; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA., Bergese P; Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.; Center for Colloid and Surface Science (CSGI), Florence, Italy.; National Center for Gene Therapy and Drugs based on RNA Technology, Padua, Italy., Bridges EM; Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK., Brucale M; Consiglio Nazionale delle Ricerche - Istituto per lo Studio dei Materiali Nanostrutturati, Bologna, Italy.; Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase, Florence, Italy., Burger D; Kidney Research Centre, Ottawa Hopsital Research Institute, Ottawa, Canada.; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada.; School of Pharmaceutical Sciences, University of Ottawa, Ottawa, Canada., Carney RP; Department of Biomedical Engineering, University of California, Davis, California, USA., Cocucci E; Division of Pharmaceutics and Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio, USA.; Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA., Crescitelli R; Sahlgrenska Center for Cancer Research, Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.; Wallenberg Centre for Molecular and Translational Medicine, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Hanser E; Department of Biomedicine, University Hospital Basel, Basel, Switzerland.; Department of Biomedicine, University of Basel, Basel, Switzerland., Harris AL; Department of Oncology, University of Oxford, Oxford, UK., Haughey NJ; Departments of Neurology and Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Hendrix A; Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium.; Cancer Research Institute Ghent, Ghent, Belgium., Ivanov AR; Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Jovanovic-Talisman T; Department of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA., Kruh-Garcia NA; Bio-pharmaceutical Manufacturing and Academic Resource Center (BioMARC), Infectious Disease Research Center, Colorado State University, Fort Collins, Colorado, USA., Ku'ulei-Lyn Faustino V; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA., Kyburz D; Department of Biomedicine, University of Basel, Basel, Switzerland.; Department of Rheumatology, University Hospital Basel, Basel, Switzerland., Lässer C; Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Lennon KM; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA., Lötvall J; Krefting Research Centre, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Maddox AL; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA., Martens-Uzunova ES; Erasmus MC Cancer Institute, University Medical Center Rotterdam, Department of Urology, Rotterdam, The Netherlands., Mizenko RR; Department of Biomedical Engineering, University of California, Davis, California, USA., Newman LA; College of Medicine and Public Health, Flinders University, Adelaide, Australia., Ridolfi A; Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands., Rohde E; Department of Transfusion Medicine, University Hospital, Salzburger Landeskliniken GmbH of Paracelsus Medical University, Salzburg, Austria.; GMP Unit, Paracelsus Medical University, Salzburg, Austria.; Transfer Centre for Extracellular Vesicle Theralytic Technologies, EV-TT, Salzburg, Austria., Rojalin T; Department of Biomedical Engineering, University of California, Davis, California, USA.; Expansion Therapeutics, Structural Biology and Biophysics, Jupiter, Florida, USA., Rowland A; College of Medicine and Public Health, Flinders University, Adelaide, Australia., Saftics A; Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA., Sandau US; Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA., Saugstad JA; Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA., Shekari F; Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.; Celer Diagnostics, Toronto, Canada., Swift S; Waipapa Taumata Rau University of Auckland, Auckland, New Zealand., Ter-Ovanesyan D; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA., Tosar JP; Universidad de la República, Montevideo, Uruguay.; Institut Pasteur de Montevideo, Montevideo, Uruguay., Useckaite Z; College of Medicine and Public Health, Flinders University, Adelaide, Australia., Valle F; Consiglio Nazionale delle Ricerche - Istituto per lo Studio dei Materiali Nanostrutturati, Bologna, Italy.; Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase, Florence, Italy., Varga Z; Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, Hungary.; Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary., van der Pol E; Amsterdam Vesicle Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands.; Biomedical Engineering and Physics, Amsterdam UMC, location AMC, University of Amsterdam, Amsterdam, The Netherlands.; Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, location AMC, University of Amsterdam, Amsterdam, The Netherlands., van Herwijnen MJC; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands., Wauben MHM; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands., Wehman AM; University of Denver, Denver, USA., Williams S; International Society for Extracellular Vesicles., Zendrini A; Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.; Center for Colloid and Surface Science (CSGI), Florence, Italy., Zimmerman AJ; Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Théry C; Institut Curie, INSERM U932, PSL University, Paris, France.; CurieCoreTech Extracellular Vesicles, Institut Curie, Paris, France., Witwer KW; Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.; EV Core Facility 'EXCEL', Institute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.; The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Publikováno v: Journal of extracellular vesicles [J Extracell Vesicles] 2024 Feb; Vol. 13 (2), pp. e12404.

Large-scale production of extracellular vesicles: Report on the "massivEVs" ISEV workshop.

Autor: Paolini L; Department of Molecular and Translational Medicine University of Brescia Brescia Italy.; Center for Colloid and Surface Science (CSGI) Department of Chemistry University of Florence Sesto Fiorentino (FI) Italy.; Department of Medical and Surgical Specialties Radiological Sciences and Public Health University of Brescia Brescia Italy., Monguió-Tortajada M; CREC Research Program Health Science Research Institute Germans Trias i Pujol (IGTP) Badalona Spain.; Heart Institute (iCor) Cardiology Department Germans Trias i Pujol University Hospital Badalona Spain., Costa M; iBET Instituto de Biologia Experimental e Tecnológica Oeiras Portugal.; Instituto de Tecnologia Química e Biológica António Xavier Universidade Nova de Lisboa Oeiras Portugal., Antenucci F; Department of Veterinary and Animal Sciences University of Copenhagen Copenhagen Denmark., Barilani M; Laboratory of Regenerative Medicine ‒ Cell Factory Department of Transfusion Medicine and Hematology Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano Italy., Clos-Sansalvador M; REMAR-IGTP Group Germans Trias i Pujol Research Institute (IGTP) & Nephrology Department University Hospital Germans Trias i Pujol (HUGTiP) Badalona (Barcelona) Catalonia Spain.; Department of Cell Biology Physiology and Immunology Universitat Autònoma de Barcelona (UAB) Bellaterra Spain., Andrade AC; Experimental and Clinical Cell Therapy Institute (ExCT) Spinal Cord Injury and Tissue Regeneration Center Paracelsus Medical University Salzburg Austria., Driedonks TAP; CDL Research University Medical Center Utrecht Utrecht University Utrecht The Netherlands.; Department of Molecular and Comparative Pathology Johns Hopkins School of Medicine Baltimore Maryland USA., Giancaterino S; Department of Civil Chemical Environmental and Materials Engineering Alma Mater Studiorum University of Bologna Bologna Italy., Kronstadt SM; Fischell Department of Bioengineering University of Maryland College Park Maryland USA., Mizenko RR; Department of Biomedical Engineering University of California Davis California USA., Nawaz M; Department of Rheumatology and Inflammation Research Institute of Medicine Sahlgrenska Academy University of Gothenburg Gothenburg Sweden., Osteikoetxea X; HCEMM-SU Extracellular Vesicles Research Group Budapest Hungary.; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Pereira C; Exogenus Therapeutics S.A. Cantanhede Portugal., Shrivastava S; Center for Gene Therapy City of Hope Beckman Research Institute and Hematological Malignancy and Stem Cell Transplantation Institute at the City of Hope Duarte California USA., Boysen AT; Department of Clinical Medicine Aarhus University Aarhus Denmark.; Department of Infectious Diseases Aarhus University Hospital Aarhus Denmark., van de Wakker SI; Department of Cardiology Experimental Cardiology Laboratory University Medical Center Utrecht Utrecht University Utrecht The Netherlands., van Herwijnen MJC; Department of Biomolecular Health Sciences Faculty of Veterinary Medicine Utrecht University Utrecht Netherlands., Wang X; Discovery Biology Discovery Sciences BioPharmaceuticals R&D AstraZeneca Gothenburg Sweden., Watson DC; University Hospitals Cleveland Medical Center Cleveland Ohio USA.; Case Western Reserve University Cleveland Ohio USA.; Cleveland Clinic Cleveland Ohio USA., Gimona M; GMP Unit Spinal Cord Injury & Tissue Regeneration Centre Salzburg (SCI-TReCS) Paracelsus Medical University Salzburg Austria.; Research Program 'Nanovesicular Therapies' Paracelsus Medical University Salzburg Austria.; Transfer Centre for Extracellular Vesicle Theralytic Technologies (EV-TT) Salzburg Austria., Kaparakis-Liaskos M; Department of Microbiology, Anatomy, Physiology and Pharmacology La Trobe University Melbourne Victoria Australia.; La Trobe Research Centre for Extracellular vesicles La Trobe University Melbourne Victoria Australia., Konstantinov K; Codiak BioSciences Cambridge Massachusetts USA., Lim SK; Exosome and Secreted Nano-vesicle Institute of Molecular & Cell Biology Singapore Singapore., Meisner-Kober N; Department of Biosciences and Medical Biology Paris-Lodron University Salzburg Hellbrunnerstrasse 34 Salzburg Austria., Stork M; Intravacc Bilthoven The Netherlands., Nejsum P; Department of Clinical Medicine Aarhus University Aarhus Denmark.; Department of Infectious Diseases Aarhus University Hospital Aarhus Denmark., Radeghieri A; Department of Molecular and Translational Medicine University of Brescia Brescia Italy.; Center for Colloid and Surface Science (CSGI) Department of Chemistry University of Florence Sesto Fiorentino (FI) Italy., Rohde E; GMP Unit Spinal Cord Injury & Tissue Regeneration Centre Salzburg (SCI-TReCS) Paracelsus Medical University Salzburg Austria.; Transfer Centre for Extracellular Vesicle Theralytic Technologies (EV-TT) Salzburg Austria.; Department of Transfusion Medicine University Hospital Salzburger Landeskliniken GesmbH (SALK) of Paracelsus Medical University Salzburg Austria., Touzet N; Centre for Environmental Research Innovation and Sustainability Institute of Technology Sligo Sligo Ireland., Wauben MHM; Department of Biomolecular Health Sciences Faculty of Veterinary Medicine Utrecht University Utrecht Netherlands., Witwer KW; Department of Molecular and Comparative Pathology Johns Hopkins School of Medicine Baltimore Maryland USA.; Department of Neurology Johns Hopkins University School of Medicine Baltimore Maryland USA., Bongiovanni A; Cell-Tech HUB and Institute for Research and Biomedical Innovation National Research Council of Italy (CNR) Palermo Italy., Bergese P; Department of Molecular and Translational Medicine University of Brescia Brescia Italy.; Center for Colloid and Surface Science (CSGI) Department of Chemistry University of Florence Sesto Fiorentino (FI) Italy.; Cell-Tech HUB and Institute for Research and Biomedical Innovation National Research Council of Italy (CNR) Palermo Italy.

Publikováno v: Journal of extracellular biology [J Extracell Biol] 2022 Oct 25; Vol. 1 (10), pp. e63. Date of Electronic Publication: 2022 Oct 25 (Print Publication: 2022).