Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Miyoko, Takahashi"'
Publikováno v:
Scanning. 18:259-268
Myoglobin and myosin light chain 1 (MLC1) are intracellular human cardiac marker proteins which are released as a consequence of ischemia. Human cardiomyocytes were isolated from fresh biopsies and also maintained for several passages in cell culture
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f42f60e5220b640d11c7fe090765899f
https://doi.org/10.1002/sca.1996.4950180401
https://doi.org/10.1002/sca.1996.4950180401
Autor:
Yoshiyuki Ohashi, Anna Villa, Miyoko Takahashi, Ikuko Hakozaki, Hiroshi Asada, Shin Kawai, S Kumaki, Masayoshi Minegishi, Shigeru Tsuchiya, Etsuko Nitanai
Publikováno v:
Blood. 98:1464-1468
Mutations of the RAG1 or RAG2 protein that eliminate their recombination activity result in T-B-severe combined immunodeficiency (SCID), whereas mutations retaining partial recombination activity lead to Omenn syndrome, a peculiar SCID characterized
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d2284b30b71ceca5197100550da682e
https://doi.org/10.1182/blood.v98.5.1464
https://doi.org/10.1182/blood.v98.5.1464
Publikováno v:
Clinical Biochemistry. 29:301-308
Objectives: To determine the serum level of fast skeletal troponin I (fsTnI) resulting from skeletal muscle damage, we have developed a sensitive two-site enzyme immunoassay to measure skeletal troponin I. Design and Methods: Twelve monoclonal antibo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab57fd1e6443d6d06b7218d3249971d5
https://doi.org/10.1016/0009-9120(96)00016-1
https://doi.org/10.1016/0009-9120(96)00016-1
Publikováno v:
Current Protocols in Molecular Biology
In the protocols in this unit, antigen is prepared for injection either by emulsifying an antigen solution with Freunds adjuvant or by homogenizing a polyacrylamide gel slice containing the protein antigen. Mice are immunized at 2- to 3-week interval
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::466cb3cf25aad1042e7ad7a474270eb7
https://pubmed.ncbi.nlm.nih.gov/18265076
https://pubmed.ncbi.nlm.nih.gov/18265076
Publikováno v:
Current Protocols in Molecular Biology
Liquid nitrogen storage is the method of choice for long-term safekeeping of hybridoma cell lines. Frozen aliquots of originally isolated hybridomas provide insurance against loss of antibody production and vigor during culture. There are many variat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34c0da95e6895ef4decafb9af4c32380
https://pubmed.ncbi.nlm.nih.gov/18265081
https://pubmed.ncbi.nlm.nih.gov/18265081
Publikováno v:
Current Protocols in Molecular Biology
Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b68b0d975b5090b124b74c66d3bc401
https://pubmed.ncbi.nlm.nih.gov/18265080
https://pubmed.ncbi.nlm.nih.gov/18265080
Publikováno v:
Current Protocols in Molecular Biology
To maximize the yield of hybrids from fusion and cloning procedures, feeder cells are required to be cocultured with the hybrids, while hybrid cell density is low. Mouse peritoneal cells, most of which are macrophages, have been found to be convenien
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b620524b4bd06033addf4898ba52ac10
https://pubmed.ncbi.nlm.nih.gov/18265078
https://pubmed.ncbi.nlm.nih.gov/18265078
Autor:
George Jackowski, Jenny Bruin, Kellie Jacks, Eric Stanton, John Marshall, Miyoko Takahashi, Lisa Barker, Weimin Zhu, Inga Kireeva, Shirley Furesz, Mila Dombrovsky, Andy Jankowski, Leslee Ingratta, Rulin Zhang, Erika Kristensen
Publikováno v:
Journal of proteome research. 3(3)
Electrophoretic and chromatographic sample preparations were compared and together detected the presence of some 600 types of protein products in human serum. Proteins from crude serum preseparated by ionic electrophoresis, chromatography, or a combi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::778efc004f1da14fc5cedbf6893d5b58
https://pubmed.ncbi.nlm.nih.gov/15253417
https://pubmed.ncbi.nlm.nih.gov/15253417
Autor:
Tammy Vrees, Rulin Zhang, Eric Stanton, Weimin Zhu, Peter Kupchak, Jason Yantha, John Marshall, Inga Kireeva, Kellie Jacks, George Jackowski, Miyoko Takahashi, Shirley Furesz, Chris Smith
Publikováno v:
Journal of proteome research. 2(4)
The MALDI-TOF spectra of peptides from the sera of normal and myocardial infarction patients produced patterns that provided an accurate diagnostic of MI. In myocardial infarction, the spectral pattern originated from the cleavage of complement C3 al
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d09ff56feb78286bc4924faa2c6632a7
https://pubmed.ncbi.nlm.nih.gov/12938926
https://pubmed.ncbi.nlm.nih.gov/12938926
Autor:
Jean-Christophe Deloulme, Jérôme Garin, Jacques Baudier, Gaëlh Ouengue Mbele, Christian Delphin, Myriam Ferro, Miyoko Takahashi, Benoit J. Gentil
Publikováno v:
The Journal of biological chemistry. 277(51)
The Zn(2+)- and Ca(2+)-binding S100B protein is implicated in multiple intracellular and extracellular regulatory events. In glial cells, a relationship exists between cytoplasmic S100B accumulation and cell morphological changes. We have identified
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e9f894e0b59c5faa855ba4a30d932e8
https://pubmed.ncbi.nlm.nih.gov/12377780
https://pubmed.ncbi.nlm.nih.gov/12377780