Visualization of multiple localizations of GLUT4 by fluorescent probes of PYP-tag with designed unnatural warhead

Autor: Miyako Nishiura, Yuichiro Hori, Maho Umeno, Kazuya Kikuchi

Publikováno v: Chemical Science.

Fluorescent probes with a designed unnatural warhead for binding to PYP-tag enabled intracellular/cell-surface selective protein labeling. This unique imaging tool was successfully applied to reveal multiple subcellular localizations of GLUT4.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::affc1d539a6da9a2abb895d289075b52
https://doi.org/10.1039/d3sc00724c

An 'OFF-ON-OFF' fluorescence protein-labeling probe for real-time visualization of the degradation of short-lived proteins in cellular systems

Autor: Shahi Imam Reja, Yuichiro Hori, Takuya Kamikawa, Kohei Yamasaki, Miyako Nishiura, Steven D. Bull, Kazuya Kikuchi

Publikováno v: Chemical science. 13(5)

The ability to monitor proteolytic pathways that remove unwanted and damaged proteins from cells is essential for understanding the multiple processes used to maintain cellular homeostasis. In this study, we have developed a new protein-labeling prob

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd0092ecd840fd7e9e1063d7d3812c25
https://pubmed.ncbi.nlm.nih.gov/35222926

SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism

Autor: Sam Benson, Antonio Fernandez, Nicole D. Barth, Fabio de Moliner, Mathew H. Horrocks, C. Simon Herrington, Jose Luis Abad, Antonio Delgado, Lisa Kelly, Ziyuan Chang, Yi Feng, Miyako Nishiura, Yuichiro Hori, Kazuya Kikuchi, Marc Vendrell

Publikováno v: Dipòsit Digital de la UB
Universidad de Barcelona
Angewandte Chemie (International Ed. in English)
Benson, S, Fernandez, A, Barth, N D, De Moliner, F, Horrocks, M H, Herrington, C S, Abad, J L, Delgado, A, Kelly, L, Chang, Z, Feng, Y, Nishiura, M, Hori, Y, Kikuchi, K & Vendrell, M 2019, ' SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism ', Angewandte Chemie, vol. 131, no. 21, pp. 6985-6989 . https://doi.org/10.1002/ange.201900465
Digital.CSIC. Repositorio Institucional del CSIC
instname

The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metaboli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f34ad2d0e518254aa03f1c0695308169
http://hdl.handle.net/2445/171908

Synthetic-Molecule/Protein Hybrid Probe with Fluorogenic Switch for Live-Cell Imaging of DNA Methylation

Autor: Isao Suetake, Miyako Nishiura, Maho Umeno, Norimichi Otomura, Yuichiro Hori, Ayuko Nishida, Kazuya Kikuchi

Publikováno v: Journal of the American Chemical Society. 140(5)

Hybrid probes consisting of synthetic molecules and proteins are powerful tools for detecting biological molecules and signals in living cells. To date, most targets of the hybrid probes have been limited to pH and small analytes. Although biomacromo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::087990a2ed5811785c56d5d70ebad32a
https://pubmed.ncbi.nlm.nih.gov/29381073