Zobrazeno 1 - 7
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pro vyhledávání: '"Miya Drury"'
Autor:
Miya Drury, Eric B. Kmiec
Publikováno v:
Nucleic Acids Research. 31:899-910
Modified single-stranded DNA oligonucleotides can direct the repair of genetic mutations in yeast, plant and mammalian cells. The mechanism by which these molecules exert their effect is being elucidated, but the first phase is likely to involve the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46ca1a069b68db675068c73e21b3eaba
https://doi.org/10.1093/nar/gkg171
https://doi.org/10.1093/nar/gkg171
Publikováno v:
Chemistry & Biology. 9(10):1073-1084
Locked nucleic acids (LNAs) are novel base modifications containing a methylene bridge uniting the 2′-oxygen and the 4′-carbon. In this study, LNA-modified single-stranded molecules directed the repair of single base mutations in a yeast chromoso
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8cff4dff601bf57319b04d7bd075a53
Publikováno v:
Oligonucleotides. 15(3)
Targeted gene repair consists of at least two major steps, the pairing of an oligonucleotide to a site bearing DNA sequence complementarity followed by a nucleotide exchange reaction directed by the oligonucleotide. In this study, oligonucleotides wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc70f2f93c7aa937eea3508b80fba0e7
https://pubmed.ncbi.nlm.nih.gov/16201904
https://pubmed.ncbi.nlm.nih.gov/16201904
Publikováno v:
Molecular biotechnology. 29(3)
Targeted gene repair uses short DNA oligonucleotides to direct a nucleotide exchange reaction at a designated site in a mammalian chromosome. The widespread use of this technique has been hampered by the inability of workers to achieve robust levels
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d377a76be7e46c507c464fe1e3d16bf5
https://pubmed.ncbi.nlm.nih.gov/15767697
https://pubmed.ncbi.nlm.nih.gov/15767697
Modified single-stranded DNA oligonucleotides can direct nucleotide exchange in Saccharomyces cerevisiae. Point and frameshift mutations are corrected in a reaction catalyzed by cellular enzymes involved in various DNA repair processes. The present m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dd4595e19ae95076079be0c47e00155
https://europepmc.org/articles/PMC133839/
https://europepmc.org/articles/PMC133839/
Publikováno v:
Molecular Therapy. 11:S146
Top of pageAbstract We have been investigating the mechanism of targeted gene repair in mammalian cells using an eGFP reporter system. The protocol involves the electroporation of single-stranded oligonucleotides into an adenocarcinoma cell line cont
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1ca1eb90de3e81b24f6faff421a70161
https://doi.org/10.1016/j.ymthe.2005.06.378
https://doi.org/10.1016/j.ymthe.2005.06.378
Autor:
Miya Drury, Eric B. Kmiec
Publikováno v:
Molecular Therapy. 9:S120
The pathway of targeted gene repair may consist of two major steps, the pairing of a short modified single-stranded oligonucleotide with the target site followed by a repair event that reverses a point mutation. In this study, we show that the level
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2110b347d637e7384c58bc22120f08be
https://doi.org/10.1016/j.ymthe.2004.06.257
https://doi.org/10.1016/j.ymthe.2004.06.257