Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Mitsuo Majima"'
Publikováno v:
Carbohydrate Research. 483:107754
Dermatan sulfate oligosaccharides having reducing end 2,5-anhydro-d-talose were prepared by partial N-deacetylation of dermatan sulfate polysaccharide with hydrazine followed by deamination with nitrous acid, and the effect of these oligosaccharides
Publikováno v:
Archives of Biochemistry and Biophysics. 506:58-65
There has been no structural information about the core protein of salmon nasal cartilage proteoglycan although its physiological activities have been investigated. Internal amino acid sequencing using nano-LC/MS/MS revealed that the salmon proteogly
Autor:
Yoshiaki Kudo, Syuichi Yoshihara, Shohei Yamagishi, Mitsuo Majima, Keiichi Takagaki, Shin-ichiro Sudo
Publikováno v:
International Congress Series. 1223:221-224
The effect of proteoglycan (PG) on colitis was examined in animal experiments using mice. The PG used was extracted from nasal cartilage of salmon head with 4% acetic acid and prepared by precipitation with ethanol followed by dialysis. The PG contai
Publikováno v:
Journal of Biochemistry. 127:695-702
A method was developed for the reconstruction of glycosaminoglycan (GAG) oligosaccharides using the transglycosylation reaction of an endo-beta-N-acetylhexosaminidase, testicular hyaluronidase, under optimal conditions. Repetition of the transglycosy
Autor:
Masahiko Endo, Hideki Matsuya, Keiichi Takagaki, Wataru Nakamura, Mitsuo Majima, Hidekazu Munakata
Publikováno v:
Glycobiology. 8:719-724
Various oligosaccharides from hyaluronic acid, which have glucuronic acid or N- acetylglucosamine at the nonreducing terminal, were prepared by digestion with a combination of testicular hyaluronidase and beta-glucuronidase. These oligo saccharides w
Publikováno v:
Nippon Eiyo Shokuryo Gakkaishi. 51:213-217
サケ鼻軟骨よりサケCSを白色粉末として調製した。次いで, このサケCSの一部をヒアルロニダーゼで消化し, その消化産物は二糖から十糖までの糖鎖長の異なる偶数オリゴ糖の混合物であ
Autor:
Mitsuo Majima, Masahiko Endo, Hozumi Narita, Hiromi Saitoh, Akihiko Matsuki, Masaharu Kasai, Keiichi Takagaki, Toshiya Nakamura
Publikováno v:
Journal of Biological Chemistry. 270:3741-3747
The reconstruction of glycosaminoglycan chains using the transglycosylation reaction of testicular hyaluronidase was investigated. First, the optimal conditions for the transglycosylation reaction catalyzed by the enzyme were determined by incubation
Autor:
Ikuko Kakizaki, Shinichiro Suto, Mito Iwafune, Mitsuo Majima, Masahiko Endo, Isoshi Nukatsuka, Keiichi Takagaki
Publikováno v:
Biochemical and biophysical research communications. 406(2)
Glycosaminoglycans were prepared as salts of different divalent cations and tested as donors in bovine testicular hyaluronidase catalyzed transglycosylation reactions. All of the metal cations examined had similar binding efficiency of divalent catio
Publikováno v:
Journal of Biological Chemistry. 265:5390-5397
Using chondroitin as a substrate, a new type of exo-beta-glucuronidase (EC 3.2.1.31) from rabbit liver was purified using a combination of ammonium sulfate fractionation, DEAE-cellulose chromatography, gel filtration on Sephracryl S-300, affinity chr
Publikováno v:
Biochemical and biophysical research communications. 270(2)
We investigated the enzymatic reconstruction of dermatan sulfate (DS) using the transglycosylation reaction of testicular hyaluronidase. First, in order to insert the IdoA-GalNAc disaccharide unit into chondroitin sulfate chains consisting of GlcA-Ga