Zobrazeno 1 - 10
of 77
pro vyhledávání: '"Mitsuo Ataka"'
Autor:
Masaru Tanokura, Keiko Kubota, Ryoichi Hirose, Mitsuo Ataka, Akihiro Yamamura, Akira Nakamura, Noriyuki Hirota, Jun Ohtsuka, Ken-ichi Miyazono, Yoriko Sawano
Publikováno v:
Crystal Growth & Design. 12:1141-1150
Space-based microgravity environments have been utilized to obtain a highly ordered crystal because of the lack of gravity-induced convection. A superconducting magnet-based quasi-microgravity is also expected to contribute to the enhancement of the
Orientation of growing crystals of Co- or Gd-containing L-threonine dehydrogenase by magnetic fields
Publikováno v:
Journal of Crystal Growth. 311:4725-4729
L-Threonine dehydrogenase from Pyrococcus horikoshii (TDH) is a water-soluble metalloenzyme, the molecular structure of which has been unknown until recently. The Zn 2+ ion in the native TDH, prepared as a recombinant protein, is replaced artificiall
Publikováno v:
Journal of Molecular Biology. 351:334-344
O-Phosphoserine sulfhydrylase is a new enzyme found in a hyperthermophilic archaeon, Aeropyrum pernix K1. This enzyme catalyzes a novel cysteine synthetic reaction from O-phospho-l-serine and sulfide. The crystal structure of the enzyme was determine
Autor:
Takanori Matsuura, Atsushi Nakagawa, Tomitake Tsukihara, Mitsuo Ataka, Shu Jie Li, Hideaki Tanaka
Publikováno v:
Journal of Crystal Growth. 276:222-229
Salt crystallizing agents studied to date generally induce a protein salting-out phenomenon at high concentrations, whereas the addition of NiCl 2 over 0.5 M induces an increase in the solubility of the tetragonal form crystals of hen egg-white lysoz
Autor:
Syou Maki, Mitsuo Ataka
Publikováno v:
Journal of Applied Physics. 96:1696-1703
Three-dimensional numerical computations of convection in water were carried out in the presence of the radial components of the magnetization force in a horizontal bore of a superconducting magnet. It is shown that the convection suppression is poss
Publikováno v:
Journal of Crystal Growth. 261:557-565
By using gadolinium chloride as a crystallizing agent, and by applying a magnetic field of 3.8 T, we could crystallize lysozyme in a floating and containerless state. Optically, these crystals had little injury on the surface. Such flawless crystals
Publikováno v:
Journal of Crystal Growth. 252:618-625
Chlorides with different paramagnetic cations such as Ni 2+ , Co 2+ and Mn 2+ were used as crystallizing agents instead of NaCl to crystallize hen egg-white lysozyme. NiCl 2 was found to give two types of crystals with different morphologies: one (ro
Publikováno v:
Journal of Crystal Growth. 247:483-496
P0 glycoprotein is the abundant membrane protein of myelin of the peripheral nervous system. We report now the statistical design of the crystallization experiments; based on our belief that important information regarding supersaturation of protein
Autor:
Mitsuo Ataka, Shinpei Tanaka
Publikováno v:
The Journal of Chemical Physics. 117:3504-3510
The phase behavior of apoferritin solutions induced by the addition of polyethylene glycol (PEG) was studied. The interaction between apoferritin molecules was determined by dynamic light scattering. The comparison of the experiments with the theoret
Publikováno v:
Journal of Crystal Growth. 242:199-208
Cation-exchange high performance liquid chromatography at pH 6, developed originally to purify human lysozyme, was applied to hen egg-white lysozyme. We could remove at least three kinds of impurities from the commercial product. The impurities were