Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Mitsuhiro Miyazawa"'
Autor:
Nana Sakata, Shigeru Shimamoto, Yuri Murakami, Orika Ashida, Toshiki Takei, Mitsuhiro Miyazawa, Yuji Hidaka
Publikováno v:
Molecules, Vol 29, Iss 22, p 5476 (2024)
To investigate the substrate recognition mechanism of trypsin-like protease cocoonase (CCN), mutational analyses were conducted at key substrate recognition sites, Asp187 and Ser188, and their effects on substrate specificity and enzymatic activity w
Externí odkaz:
https://doaj.org/article/9728db39e7354480bf6a18ae9ed602b3
Autor:
Hiroyuki Kajiura, Ken-ichiro Tatematsu, Tsuyoshi Nomura, Mitsuhiro Miyazawa, Akihiro Usami, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-15 (2022)
Abstract The silkworm, Bombyx mori, is an attractive host for recombinant protein production due to its high expression efficiency, quality, and quantity. Two expression systems have been widely used for recombinant protein production in B. mori: bac
Externí odkaz:
https://doaj.org/article/9b9a16dad20a4bc99efd5c515a91f0db
Publikováno v:
Molecules, Vol 28, Iss 8, p 3494 (2023)
Investigations of protein folding have largely involved the use of disulfide-containing proteins, since the disulfide-coupled folding of proteins allows folding intermediates to be trapped and their conformations determined. However, studies of the f
Externí odkaz:
https://doaj.org/article/2aa2171738c94356b9fcfe7dcdba4c7d
Autor:
Nana Sakata, Ayumi Ogata, Mai Takegawa, Yuri Murakami, Misaki Nishimura, Mitsuhiro Miyazawa, Teruki Hagiwara, Shigeru Shimamoto, Yuji Hidaka
Publikováno v:
Molecules, Vol 27, Iss 22, p 8063 (2022)
Cocoonase is folded in the form of a zymogen precursor protein (prococoonase) with the assistance of the propeptide region. To investigate the role of the propeptide sequence on the disulfide-coupled folding of cocoonase and prococoonase, the amino a
Externí odkaz:
https://doaj.org/article/140ebaf9a0f6401a80ba73e688697467
Publikováno v:
Entomologia Experimentalis et Applicata. 171:421-434
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::71d77b387c4ab83caed3c0791e6462c5
https://doi.org/10.1111/eea.13300
https://doi.org/10.1111/eea.13300
The propeptide sequence assists the correct folding required for the enzymatic activity of cocoonase
Autor:
Nana Sakata, Ayumi Ogata, Mai Takegawa, Nagisa Tajima, Misaki Nishimura, Teruki Hagiwara, Mitsuhiro Miyazawa, Shigeru Shimamoto, Yuji Hidaka
Publikováno v:
Biochemical and biophysical research communications. 624
Cocoonase, a protein that is produced by the silkworm (Bombyx mori), is thought to specifically digest the sericin protein of the cocoon and has a high homology with trypsin. Similar to trypsin, cocoonase is folded as an inactive precursor protein wh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74e90752fea8dab5a4509b7e2f82711f
https://pubmed.ncbi.nlm.nih.gov/35932577
https://pubmed.ncbi.nlm.nih.gov/35932577
Publikováno v:
Analytical Sciences. 33:121-123
The molecular structural analysis of capture thread, including its viscid droplets of oriental golden orb-web spider Nephila clavata, has been performed by microscopic FT-IR spectroscopy. The obtained spectra of capture threads with and without visci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1178d4961bc1b9532a8f4091d810886
https://doi.org/10.2116/analsci.33.121
https://doi.org/10.2116/analsci.33.121
Publikováno v:
KOBUNSHI RONBUNSHU. 72:773-776
近年，天然多糖類であるアルギン酸やキトサンなどにタンパク質やペプチドなどを修飾した，組織工学用の材料の設計が注目されている．アルギン酸-カルシウムヒドロゲルは，分解時に
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b98bf7b801f420ea8547ef3faf92addb
https://doi.org/10.1295/koron.2015-0030
https://doi.org/10.1295/koron.2015-0030
Autor:
Mitsuhiro Miyazawa
Publikováno v:
2016 International Congress of Entomology.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a1ecfc079d38df1839376933f0198ff4
https://doi.org/10.1603/ice.2016.114357
https://doi.org/10.1603/ice.2016.114357
Publikováno v:
Journal of Molecular Structure. 974:179-182
The hydration structure of starch molecule in Domyoji-ko, which is made from gluey rice, was investigated by hetero 2D correlation analysis of IR and NIR spectroscopy. The feature near 1020 cm −1 in the IR spectra of Domyoji-ko is changed by rehydr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::63eabf649bd6956b3be8274de727dbec
https://doi.org/10.1016/j.molstruc.2010.02.074
https://doi.org/10.1016/j.molstruc.2010.02.074