Chemoenzymatic Elaboration of Monosaccharides using Engineered Cytochrome $P450_{BM3} $ Demethylases

Autor: Lewis, Jared C., Bastian, Sabine, Bennett, Clay S., Fu, Yu, Mitsuda, Yuuichi, Chen, Mike M., Greenberg, William A., Wong, Chi-Huey, Arnold, Frances H.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 2009 Sep . 106(39), 16550-16555.

Externí odkaz: https://www.jstor.org/stable/40484942

Large-scale production of functional human adrenomedullin: expression, cleavage, amidation, and purification

Autor: Kazuo Kitamura, Kenji Mitsushima, Shigeki Kamitani, Mitsuda Yuuichi, Akio Takimoto, Tsuneaki Sakata

Publikováno v: Protein Expression and Purification. 25:448-455

Human adrenomedullin (hAM) is a 52-amino-acid regulatory peptide containing a six-membered ring structure and an amidated C-terminus, features that are essential for its biological activity. Here, we describe a simple and effective protocol for produ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f798ce21d872d3d8c9f009b2dc424c08
https://doi.org/10.1016/s1046-5928(02)00030-x

Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases.

Autor: Lewis, Jared C., Bastian, Sabine, Bennett, Clay S., Yu Fu, Mitsuda, Yuuichi, Chena, Mike M., Greenberg, William A., Chi-Huey Wong, Arnold, Frances H.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America; 9/29/2009, Vol. 106 Issue 39, p16550-16555, 6p, 3 Diagrams, 2 Graphs