Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Mitko I. Dimitrov"'
Autor:
Mitko I. Dimitrov, Vladimir I Getov, A. Ch. Shosheva, Nedyalka P Terezova, S. D. Stoichev, Anthony A. Donchev
Publikováno v:
Russian Journal of Plant Physiology. 58:181-184
Changes in the content of isoplastocyanins a (PCa) and b (PCb) in Populus nigra leaves were studied during complete vegetation cycle (from April 30 to October 28). Measurements were made every 7 days. The procedure included extraction and purificatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1fa11546c814056eb7b5344f5b3145c1
https://doi.org/10.1134/s1021443711010055
https://doi.org/10.1134/s1021443711010055
Autor:
Mitko I. Dimitrov, Anthony A. Donchev, Nedyalka P Terezova, Svetozar D Stoichev, Alexandra Shosheva, Vladimir I Getov
Publikováno v:
Zeitschrift für Naturforschung C. 65:225-230
An improved four-stage isolation and purification procedure for preparing poplar isoplastocyanins is described in detail. Absorbance (UV-VIS) spectroscopy and isoelectric focusing (IEF) are used to determine the protein purity and identity. The prese
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34d428175c29a333f79dc778f087e3ab
https://doi.org/10.1515/znc-2010-3-410
https://doi.org/10.1515/znc-2010-3-410
Publikováno v:
Journal of Thermal Analysis and Calorimetry. 98:877-883
We attempted to determine the experimental conditions under which poplar plastocyanin iso-forms PCa and PCb undergo reversible thermal unfolding studied by differential scanning calorimetry (DSC). Our results indicate that an exothermic unfolding pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::06831341a41b99a9a0be620e02941de6
https://doi.org/10.1007/s10973-009-0141-1
https://doi.org/10.1007/s10973-009-0141-1
Publikováno v:
Zeitschrift für Naturforschung C. 64:399-404
The redox potentials of poplar plastocyanins a and b (PCa, PCb) were determined by spectro photometric titrations of their reduced forms with [Fe(CN)6]3-. It was found that the two isoforms have the following millimolar extinction coefficients ε597,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d107abc6686b0acde18a528811951ebd
https://doi.org/10.1515/znc-2009-5-617
https://doi.org/10.1515/znc-2009-5-617
Autor:
V. Getov, Alexandra Shosheva, I Zlatanov, Emil Alexov, G. Toromanov, Anthony A. Donchev, Mitko I. Dimitrov
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1698:67-75
A detailed study of the tyrosine spectral characteristics was carried out in a broad range of pHs for both isoforms of plastocyanin from poplar. It was found that Tyr 80 is always protonated while Tyr 83 can form a tirosinate at high pHs. The p K a o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb5b90322ee77753cd91202ba228f8c1
https://doi.org/10.1016/j.bbapap.2003.10.010
https://doi.org/10.1016/j.bbapap.2003.10.010
Publikováno v:
Journal of Applied Microbiology. 93:751-757
Aims: To evaluate the lytic activities of crude enzymes from Cytophaga sp. LR2 on Rhodella reticulata cells and isolated algal polysaccharide. Methods and Results: The Cytophaga compartment was separated after centrifugation in a cell suspension for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::113f7b2cb6c403a41aab1d0be494474e
https://doi.org/10.1046/j.1365-2672.2002.01745.x
https://doi.org/10.1046/j.1365-2672.2002.01745.x
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1463:429-438
The effect of electrostatic interactions on the conformation and thermal stability of plastocyanin (Pc) was studied by infrared spectroscopy. Association of any of the two redox states of the protein with positively charged membranes at neutral pH do
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4be397cb154215c8b42f9ae358e1f6c3
https://doi.org/10.1016/s0005-2736(99)00230-8
https://doi.org/10.1016/s0005-2736(99)00230-8
Autor:
Hans D. Bartunik, Mitko I. Dimitrov, Galina S. Kachalova, Anthony A. Donchev, Alexandra Shosheva, Gleb Bourenkov
Publikováno v:
Journal of inorganic biochemistry. 115
Plastocyanin (PC) from poplar leaves is present in two isoforms, PCa and PCb, which differ in sequence by amino acid replacements at locations remote from the copper center and simultaneously act in the photosynthetic electron-transport chain. We des
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e359ffe33b2a667c879c3a4274840989
https://pubmed.ncbi.nlm.nih.gov/22883960
https://pubmed.ncbi.nlm.nih.gov/22883960
Autor:
Z. Lalchev, Mitko I. Dimitrov, Vladimir I Getov, Asya Tsanova, G. As. Georgiev, Albena Jordanova, Sl. Ivanova
Publikováno v:
Biochemical and biophysical research communications. 419(4)
The interaction of monogalactosyldiacylglycerol (MGDG) with cytochrome b 6 f complex (cyt b 6 f ), a major component of the photosynthetic apparatus, was studied in Langmuir monolayers during compression/expansion cycling and at constant surface pres
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f1f38ffcf868af76cedbfee860403ea
https://pubmed.ncbi.nlm.nih.gov/22374069
https://pubmed.ncbi.nlm.nih.gov/22374069
Autor:
Petya K, Christova, Anthony A, Donchev, Alexandra Ch, Shosheva, Vladimir I, Getov, Mitko I, Dimitrov
Publikováno v:
Indian journal of biochemistrybiophysics. 46(5)
Oxidation of oxymyoglobin [MbO2(Fe2+)] by isoplastocyanins a (PCa) and b (PCb) was experimentally investigated and the corresponding redox reaction was modeled using the physicochemical parameters of the isoforms to study the effect of the dimorphism
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9a38b85e07b98a98e8eb8b42b5331c8d
https://pubmed.ncbi.nlm.nih.gov/20027868
https://pubmed.ncbi.nlm.nih.gov/20027868