Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Mitchell G. Kluesner"'
Autor:
Mitchell G. Kluesner, Rafail Nikolaos Tasakis, Taga Lerner, Annette Arnold, Sandra Wüst, Marco Binder, Beau R. Webber, Branden S. Moriarity, Riccardo Pecori
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 25, Iss , Pp 515-523 (2021)
We present MultiEditR (Multiple Edit Deconvolution by Inference of Traces in R), the first algorithm specifically designed to detect and quantify RNA editing from Sanger sequencing (z.umn.edu/multieditr). Although RNA editing is routinely evaluated b
Externí odkaz:
https://doaj.org/article/6ae1106ace65443689c12fb1b0a3e6cd
Autor:
Mitchell G. Kluesner, Walker S. Lahr, Cara-lin Lonetree, Branden A. Smeester, Xiaohong Qiu, Nicholas J. Slipek, Patricia N. Claudio Vázquez, Samuel P. Pitzen, Emily J. Pomeroy, Madison J. Vignes, Samantha C. Lee, Samuel P. Bingea, Aneesha A. Andrew, Beau R. Webber, Branden S. Moriarity
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Base editors can inactivate splice sites or introduce stop codons into a gene sequence. Here the authors present SpliceR to design, rank, and test sgRNAs for efficient gene disruption in T cells.
Externí odkaz:
https://doaj.org/article/66661828f7814bdf8c8c049d29a4b13b
Autor:
Beau R. Webber, Cara-lin Lonetree, Mitchell G. Kluesner, Matthew J. Johnson, Emily J. Pomeroy, Miechaleen D. Diers, Walker S. Lahr, Garrett M. Draper, Nicholas J. Slipek, Branden A. Smeester, Klaus N. Lovendahl, Amber N. McElroy, Wendy R. Gordon, Mark J. Osborn, Branden S. Moriarity
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.
Externí odkaz:
https://doaj.org/article/7b8f9319f4d349b9a42406e609bf1cd4
Autor:
Christopher J. Sipe, Mitchell G. Kluesner, Samuel P. Bingea, Walker S. Lahr, Aneesha A. Andrew, Minjing Wang, Anthony P. DeFeo, Timothy L. Hinkel, Kanut Laoharawee, John E. Wagner, Margaret L. MacMillan, Gregory M. Vercellotti, Jakub Tolar, Mark J. Osborn, R. Scott McIvor, Beau R. Webber, Branden S. Moriarity
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 15, p 8416 (2022)
Fanconi anemia (FA) is a rare genetic disease in which genes essential for DNA repair are mutated. Both the interstrand crosslink (ICL) and double-strand break (DSB) repair pathways are disrupted in FA, leading to patient bone marrow failure (BMF) an
Externí odkaz:
https://doaj.org/article/255cd0436495493cbbacdc43a4d8450a
Autor:
Beau R. Webber, Cara-lin Lonetree, Mitchell G. Kluesner, Matthew J. Johnson, Emily J. Pomeroy, Miechaleen D. Diers, Walker S. Lahr, Garrett M. Draper, Nicholas J. Slipek, Branden A. Smeester, Klaus N. Lovendahl, Amber N. McElroy, Wendy R. Gordon, Mark J. Osborn, Branden S. Moriarity
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-1 (2019)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Externí odkaz:
https://doaj.org/article/e60ca323ac3d418e8abfbe4a94ebc9be
Autor:
Kelsie L. Becklin, Garrett M. Draper, Rebecca A. Madden, Mitchell G. Kluesner, Tomoyuki Koga, Miller Huang, William A. Weiss, Logan G. Spector, David A. Largaespada, Branden S. Moriarity, Beau R. Webber
Publikováno v:
The CRISPR journal, vol 5, iss 4
CRISPR J
CRISPR J
Advances in genome and tissue engineering have spurred significant progress and opportunity for innovation in cancer modeling. Human induced pluripotent stem cells (iPSCs) are an established and powerful tool to study cellular processes in the contex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da020215660abed7a4b360505fb303f3
https://doi.org/10.1089/crispr.2022.0032
https://doi.org/10.1089/crispr.2022.0032
Autor:
Morito Kurata, Natalie K Wolf, Walker S Lahr, Madison T Weg, Mitchell G Kluesner, Samantha Lee, Kai Hui, Masano Shiraiwa, Beau R Webber, Branden S Moriarity
Publikováno v:
PLoS ONE, Vol 13, Iss 9, p e0198714 (2018)
The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism of adaptive immunity in bacteria. This system has been adopted for numerous genome engineering applications in research and recently, therapeutics. The CRISPR/Cas9 sy
Externí odkaz:
https://doaj.org/article/5ae8af2eafd846b1ad332b032c8f37da
Autor:
Marco Binder, Sandra Wüst, Riccardo Pecori, Mitchell G. Kluesner, Taga Lerner, Annette Arnold, Branden S. Moriarity, Beau R. Webber, Rafail Nikolaos Tasakis
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 25, Iss, Pp 515-523 (2021)
Molecular Therapy. Nucleic Acids
Molecular Therapy. Nucleic Acids
We present MultiEditR (Multiple Edit Deconvolution by Inference of Traces in R), the first algorithm specifically designed to detect and quantify RNA editing from Sanger sequencing (z.umn.edu/multieditr). Although RNA editing is routinely evaluated b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62dabb259e5d0aa64c541f4495b64c4c
http://www.sciencedirect.com/science/article/pii/S2162253121001761
http://www.sciencedirect.com/science/article/pii/S2162253121001761
Autor:
Aneesha A. Andrew, Emily J. Pomeroy, Xiaohong Qiu, Mitchell G. Kluesner, Walker S. Lahr, Cara lin Lonetree, Branden A. Smeester, Madison J. Vignes, Branden S. Moriarity, Samuel P. Pitzen, Nicholas J. Slipek, Samantha C. Lee, Samuel P. Bingea, Patricia N. Claudio Vázquez, Beau R. Webber
Publikováno v:
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
CRISPR-Cas9 cytidine and adenosine base editors (CBEs and ABEs) can disrupt genes without introducing double-stranded breaks by inactivating splice sites (BE-splice) or by introducing premature stop (pmSTOP) codons. However, no in-depth comparison of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::343a074f434d56ae670a5db2b2468692
http://europepmc.org/articles/PMC8065034
http://europepmc.org/articles/PMC8065034
Autor:
Mitchell G. Kluesner, Rafail Nikolaos Tasakis, Branden S. Moriarity, Riccardo Pecori, F. Nina Papavasiliou, Taga Lerner
Publikováno v:
Methods in Molecular Biology ISBN: 9781071607862
The AID/APOBEC family of enzymes are cytidine deaminases that act upon DNA and RNA. Among APOBECs, the best characterized family member to act on RNA is the enzyme APOBEC1. APOBEC1-mediated RNA editing plays a key role in lipid metabolism and in main
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::08ce9d8c94f312fb45772fff1c868317
https://doi.org/10.1007/978-1-0716-0787-9_4
https://doi.org/10.1007/978-1-0716-0787-9_4
