Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Misao Ide"'
Publikováno v:
Biomedical Research. 16:303-309
Publikováno v:
FEBS Letters. 350:99-103
Conditioned media of human glial cell lines induced alkaline phosphatase activity in cultured calf artery endothelial cells. The maximal alkaline phosphatase activity in the culture was comparable to the level in isolated brain capillary endothelial
Autor:
Shin Takagi, Kiyoshi Ezumi, Yumi Kosada, Satoshi Hata, Shigenori Harada, Hiroshi Takemoto, Misao Ide, Shinji Nishimura, Susumu Hosoi
Publikováno v:
Microbiology and Immunology. 38:63-71
Anti-human IgE monoclonal antibodies (mAbs) were produced and eight clones recognizing epitopes on native IgE were selected. Epitopes were mapped by a competitive inhibition enzyme-linked immunosorbent assay, Western blotting and a multi-pin peptide
Publikováno v:
Immunological Investigations. 20:461-474
A continuous cloned cell line (Y479) was established by culturing normal mouse spleen cells in a high concentration of interleukin-2 (IL-2). Y479 cells showed morphological characteristics of large granular lymphocyte with the phenotypes of Thy-1.2+,
Publikováno v:
Journal of Clinical Biochemistry and Nutrition. 11:79-89
Publikováno v:
In vitro cellulardevelopmental biology : journal of the Tissue Culture Association. 26(6)
The present study deals with a rapid and convenient assay for blood-brain barrier (BBB)-associated enzymes, gamma-glutamyl transpeptidase (gamma-GTP) and alkaline phosphatase (ALP), in cultured endothelial cells and other cells. These enzyme activiti
Publikováno v:
European Journal of Pharmacology. 160:313-321
We compared the properties of vascular and platelet thromboxane A2/prostaglandin H2 receptors in the pig. The binding profiles of U46619, several prostaglandins and thromboxane A2/prostaglandin H2 receptor antagonists to the aorta and platelet recept
Publikováno v:
Journal of Immunological Methods. 121:281-288
A fluorescence sandwich ELISA was developed for the quantitation of human interleukin-2 (IL-2) using a combination of two anti-human IL-2 antibodies, L61 and biotinylated anti-IL-2 rabbit IgG. The minimum level of IL-2 detectable by this ELISA was 0.
Autor:
Motohiko Ueda, Masafumi Fujimoto, Masami Doteuchi, Seijiro Hara, Misao Ide, Tadashi Okabayashi, Shin-ichi Mihara
Publikováno v:
European Journal of Pharmacology. 151:59-65
The binding characteristics of [3H]U46619, a tritiated thromboxane A2/prostaglandin H2 mimetic, were studied in pig aorta smooth muscle membranes. Binding was fast, saturable, selective and reversible. The KD values determined by kinetics, equilibriu
Publikováno v:
FEBS Letters. 242:53-56
Five monoclonal antibodies (L15, L20, L23, L34, and L61) against human recombinant interleukin-2 were tested for their effects on the interleukin-2 bioactivity and binding. Four of these monoclonal antibodies, L15, L20, L34, and L61, which had neutra