Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Mirko Palla"'
Autor:
Sukanya Punthambaker, Stefan Roever, James Pollard, Timothy Craig, Adam Qwan, Preethi Sarvabhowman, Shaw Sun, Andrew Trans, Jeff Nivala, Sara Kelley, Eileen Thai, Mirko Palla, P. Benjamin Stranges, Daniel J. Wiegand, Ilya Lederman, Frederic Vigneault, Aruna Ayer, Colin Mcgaw, Helen Franklin, Charles Schwab, George M. Church, Evan Amato, Dhruti Dalal, Cleoma Arnold, Carl W. Fuller, Dmitriy Gremyachinskiy, Meng Taing
DNA polymerases have revolutionized the biotechnology field due to their ability to precisely replicate stored genetic information. Screening variants of these enzymes for unique properties gives the opportunity to identify polymerases with novel fea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7aadfe057b29ffe39fde4476cc3c707
https://doi.org/10.1101/2020.03.15.993071
https://doi.org/10.1101/2020.03.15.993071
Autor:
James Pollard, Zengmin Li, Ashwini Bhat, Andrew Trans, Jennifer Hovis, Minchen Chien, Cleoma Arnold, Edward Shian Liu, Arek Bibillo, Randy Davis, John J. Kasianowicz, Mirko Palla, James J. Russo, Mintu Porel, Irina Morozova, Wenjing Guo, Jingyue Ju, Shundi Shi, Cynthia Cech, Shiv Kumar, Eric Takeshi Harada, Chuanjuan Tao, Carl W. Fuller, Alexander Yang, Michael Dorwart, Stefan Roever, Roger Chen, Anne Aguirre, George M. Church, Daniel Korenblum, P. Benjamin Stranges, Sergey Kalachikov
Publikováno v:
Proceedings of the National Academy of Sciences. 113:5233-5238
DNA sequencing by synthesis (SBS) offers a robust platform to decipher nucleic acid sequences. Recently, we reported a single-molecule nanopore-based SBS strategy that accurately distinguishes four bases by electronically detecting and differentiatin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b27664d67e1e4e83ff1df64c96ad84c2
https://doi.org/10.1073/pnas.1601782113
https://doi.org/10.1073/pnas.1601782113
Autor:
Filippo Bosco, Michael Stenbæk Schmidt, Jingyue Ju, Tomas Rindzevicius, Qiao Lin, Anja Boisen, Tommy Sonne Alstrøm, Jaeyoung Yang, Mirko Palla
Publikováno v:
RSC Advances. 5:85845-85853
Surface-enhanced Raman spectroscopy (SERS) based on nanostructured platforms is a promising technique for quantitative and highly sensitive detection of biomolecules in the field of analytical biochemistry. Here, we report a mathematical model to pre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71fa588bb6bbd3e3728b5eeffeddd014
https://doi.org/10.1039/c5ra16108h
https://doi.org/10.1039/c5ra16108h
Autor:
Steffen Jockusch, Mirko Palla, Nicholas J. Turro, James J. Russo, Wenjing Guo, Jian Wu, Cheng Guo, Shundi Shi, Jingyue Ju, Zengmin Li
Publikováno v:
RSC Adv.. 4:49342-49346
As an alternative to fluorescence-based DNA sequencing by synthesis (SBS), we report here an approach using an azido moiety (N3) that has an intense, narrow and unique Raman shift at 2125 cm−1, where virtually all biological molecules are transpare
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c564da734c0acdbc001912386ca0f50
https://doi.org/10.1039/c4ra08398a
https://doi.org/10.1039/c4ra08398a
Publikováno v:
RSC Adv.. 4:4269-4277
Single-nucleotide polymorphisms (SNPs) are the most abundant type of genetic variations; they provide the genetic fingerprint of individuals and are essential for genetic biomarker discoveries. Accurate detection of SNPs is of great significance for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b993b62bd983324a9e51b0bfc32becb8
https://doi.org/10.1039/c3ra44091e
https://doi.org/10.1039/c3ra44091e
Publikováno v:
Journal of Biomolecular Structure and Dynamics. 31:129-141
Hepatitis C virus (HCV) NS3 helicase couples adenosine triphosphate (ATP) binding and hydrolysis to polynucleotide unwinding. Understanding the regulation mechanism of ATP binding will facilitate targeting of the ATP-binding site for potential therap
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b753376116ed11d6da7b3aa4016d2216
https://doi.org/10.1080/07391102.2012.698236
https://doi.org/10.1080/07391102.2012.698236
Autor:
Shiv Kumar, James Pollard, Alexander Yang, Sergey Kalachikov, Mirko Palla, Minchen Chien, Mintu Porel, Jeff Nivala, Cleoma Arnold, Chuanjuan Tao, Roger Chen, Dmitriy Gremyachinskiy, Shundi Shi, Michael Dorwart, Randy Davis, Zengmin Li, Daniel Korenblum, Eric Takeshi Harada, Aman S. Aberra, P. Benjamin Stranges, James J. Russo, Arek Bibillo, Jingyue Ju, Carl W. Fuller, Stefan Roever, Anne Aguirre, Andrew Trans, George M. Church, Irina Morozova, Ashwini Bhat
Publikováno v:
Proceedings of the National Academy of Sciences. 113
Scalable, high-throughput DNA sequencing is a prerequisite for precision medicine and biomedical research. Recently, we presented a nanopore-based sequencing-by-synthesis (Nanopore-SBS) approach, which used a set of nucleotides with polymer tags that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bba483ebd00f0c955c9e558e08fe7298
https://doi.org/10.1073/pnas.1608271113
https://doi.org/10.1073/pnas.1608271113
Autor:
Jingyue Ju, Steffen Jockusch, James J. Russo, Tomas Rindzevicius, Shiv Kumar, Mirko Palla, Tommy Sonne Alstrøm, Zengmin Li, Anja Boisen, Filippo Bosco, Michael Stenbæk Schmidt
Publikováno v:
2016 IEEE SENSORS.
We describe here a novel surface-enhanced Raman spectroscopy (SERS) based technique for monitoring the conjugation of small molecules by the well-known click reaction between an alkyne and azido moiety on the partner molecules. The monitoring princip
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::72ed8ef74d4900f61a616878086444fc
https://doi.org/10.1109/icsens.2016.7808595
https://doi.org/10.1109/icsens.2016.7808595
Autor:
Tomas Rindzevicius, Filippo Bosco, Tommy Sonne Alstrøm, Qiao Lin, Anja Boisen, Jingyue Ju, Michael Stenbak Schmidt, Mirko Palla, Jaeyoung Yang
Publikováno v:
2015 IEEE SENSORS.
This paper presents the development of a novel statistical method for quantifying trace amounts of biomolecules by surface-enhanced Raman spectroscopy (SERS) using a rigorous, single molecule (SM) theory based mathematical derivation. Our quantificat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8b453823c9d1fc2b5319a91517a06f9d
https://doi.org/10.1109/icsens.2015.7370388
https://doi.org/10.1109/icsens.2015.7370388
Publikováno v:
Sensors and actuators. A, Physical. 195
Genotyping of single nucleotide polymorphisms (SNPs) allows diagnosis of human genetic disorders associated with single base mutations. Conventional SNP genotyping methods are capable of providing either accurate or high-throughput detection, but are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::858d0f6ac00b48d2e911ddaf82ebb297
https://pubmed.ncbi.nlm.nih.gov/24729659
https://pubmed.ncbi.nlm.nih.gov/24729659