Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Mireia Uranga"'
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
CRISPR–Cas technologies contribute to enhancing our understanding of plant gene functions, and to the precise breeding of crop traits. Here, we review the latest progress in plant genome editing, focusing on emerging CRISPR–Cas systems, DNA-free
Externí odkaz:
https://doaj.org/article/b9dcae64a1684f6c9be677b06edb5dec
Autor:
Mireia Uranga, José‐Antonio Daròs
Publikováno v:
The Plant Genome, Vol 16, Iss 2, Pp n/a-n/a (2023)
Abstract The recent emergence of tools based on the clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR‐associated (Cas) proteins have revolutionized targeted genome editing, thus holding great promise to both basic plan
Externí odkaz:
https://doaj.org/article/9da9d811c2d2429ca295810fe82f0afb
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102091- (2023)
Summary: Viral vectors hold enormous potential for genome editing in plants as transient delivery vehicles of CRISPR-Cas components. Here, we describe a protocol to assemble plant viral vectors for single-guide RNA (sgRNA) delivery. The obtained vira
Externí odkaz:
https://doaj.org/article/6ee7e044fe9546139f3f9b1a4fc0b763
Autor:
Sara Selma, Silvia Gianoglio, Mireia Uranga, Marta Vázquez‐Vilar, Ana Espinosa‐Ruiz, Margit Drapal, Paul D. Fraser, José‐Antonio Daròs, Diego Orzáez
Publikováno v:
The Plant Journal. 111:1550-1564
Programmable transcriptional regulators based on CRISPR architecture are promising tools for the induction of plant gene expression. In plants, CRISPR gene activation is effective with respect to modulating development processes, such as the flowerin
SummaryViral vectors hold enormous potential for genome editing in plants as transient delivery vehicles of CRISPR-Cas components. Here, we describe a protocol to assemble plant viral vectors for single guide RNA (sgRNA) delivery. The obtained viral
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b240b5e32c021d52ecad012e73c19b9b
https://doi.org/10.1101/2022.09.07.507055
https://doi.org/10.1101/2022.09.07.507055
Autor:
Verónica Aragonés, Sara Selma, José-Antonio Daròs, Diego Orzaez, Marta Vazquez-Vilar, Mireia Uranga
Publikováno v:
The Plant Journal
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
Digital.CSIC. Repositorio Institucional del CSIC
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
Digital.CSIC. Repositorio Institucional del CSIC
SUMMARY Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR‐associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR‐Cas strategies in plants rel
Autor:
Mireia Uranga, José-Antonio Daròs
Publikováno v:
The plant genomeREFERENCES.
The recent emergence of tools based on the clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins have revolutionized targeted genome editing, thus holding great promise to both basic plant science a
Autor:
Camilo Calvache, Diego Orzaez, José-Antonio Daròs, Mireia Uranga, Sara Selma, Marta Vazquez-Vilar, Asun Fernández-del-Carmen
Publikováno v:
Plant Biotechnology Journal
CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti-CRISPR proteins as tools to prevent CRISPR/Cas-mediated gene editing and gene activation in plants has not been explored yet. This study describes the characterizati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dad5495c612f4b95fd2b2a854dcc4d8e
http://hdl.handle.net/10261/304754
http://hdl.handle.net/10261/304754
Autor:
Mireia Uranga, Sara Selma, Camilo Calvache, José-Antonio Daròs, Marta Vazquez-Vilar, Diego Orzaez
This study describes the strong anti-CRISPR activity of the bacterial AcrIIA4 protein in Nicotiana benthamiana, a model plant used as molecular farming platform. The results demonstrate that AcrIIA4 abolishes site-directed mutagenesis in leaves when
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::faae5158666111a92c5f43adb7e25dfd
https://doi.org/10.21203/rs.3.rs-143630/v1
https://doi.org/10.21203/rs.3.rs-143630/v1