Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Miranda Gek Sim Yap"'
Publikováno v:
Journal of Proteome Research. 12:3496-3510
Chinese hamster ovary (CHO) cell lines are widely used for the expression of therapeutic recombinant proteins, including monoclonal antibodies and other biologics. For manufacturing, cells derived from a single-cell clone are typically used to ensure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7f95c203c237ec2ec80c28d2309fac4
https://doi.org/10.1021/pr400352n
https://doi.org/10.1021/pr400352n
Publikováno v:
Biotechnology and Bioengineering. 107:516-528
One of the goals of recombinant glycoprotein production is to achieve consistent glycosylation. Although many studies have examined the changes in the glycosylation quality of recombinant protein with culture, very little has been done to examine the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e95ad8dabec51d9cd76ac9fb0e580335
https://doi.org/10.1002/bit.22828
https://doi.org/10.1002/bit.22828
Publikováno v:
Molecules and Cells. 27:577-582
The development of a high-throughput functional genomic screening provides a novel and expeditious approach in identifying critical genes involved in specific biological processes. Here we describe a cell-based cDNA screening system to identify the t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4df78b2ef526ed265b88cefd7799289f
https://doi.org/10.1007/s10059-009-0078-z
https://doi.org/10.1007/s10059-009-0078-z
Publikováno v:
Enzyme and Microbial Technology. 39:391-398
DNA plasmids of Escherichia coli are common vectors for recombinant protein and metabolite production and have potential therapeutic applications as genetic vaccines and therapeutics. However, plasmid maintenance imposes a metabolic burden on the hos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0942d39a0bf7c5758ba5eaf9bed31f92
https://doi.org/10.1016/j.enzmictec.2005.11.048
https://doi.org/10.1016/j.enzmictec.2005.11.048
Publikováno v:
Biotechnology and Bioengineering. 95:350-361
Based on the transcriptional profiling of CHO cell culture using microarray, four key early apoptosis signaling genes, Fadd, Faim, Alg-2, and Requiem, were identified and CHO GT (Gene Targeted) cell lines were developed by targeting these four genes.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b2475d634e948eb71b080903c167b53
https://doi.org/10.1002/bit.20871
https://doi.org/10.1002/bit.20871
Autor:
Miranda Gek Sim Yap, Yih Yean Lee, Danny C.F. Wong, Chew-Kiat Heng, Kathy Wong, Peter Nissom Morin
Publikováno v:
Biotechnology and Bioengineering. 94:373-382
Chinese Hamster ovary (CHO) cells are regarded as one of the "work-horses" for complex biotherapeutics production. In these processes, loss in culture viability occurs primarily via apoptosis, a genetically controlled form of cellular suicide. Using
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f490fefc906bd457e8b6edd0ffdc3f58
https://doi.org/10.1002/bit.20872
https://doi.org/10.1002/bit.20872
Autor:
Miranda Gek Sim Yap, Lin-Tang Goh
Publikováno v:
Journal of Separation Science. 28:2104-2110
A robust tandem HPLC method coupling size-exclusion (Shodex Asahipak GS-320HQ) and reversed phase (Vydac 218TP54) columns with ultraviolet detection was developed for quantitative determination of interferon-gamma (IFN-gamma) in Chinese hamster ovary
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb48ede659acc3fb4166bfb6ae15ffeb
https://doi.org/10.1002/jssc.200500045
https://doi.org/10.1002/jssc.200500045
Publikováno v:
Biotechnology and Bioengineering. 89:164-177
As we pursue the means to improve yields to meet growing therapy demands, it is important to examine the impact of process control on glycosylation patterns to ensure product efficacy and consistency. In this study, we describe a dynamic on-line fed-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48f313e4d28b0b7a54e24ea337ef684f
https://doi.org/10.1002/bit.20317
https://doi.org/10.1002/bit.20317
Autor:
Corrine Wan, Hock Chuan Yeo, Sze Wai Ng, Chung Ping Chow, Faraaz N.K. Yusufi, Ai Ping Hiu, Ju Xin Chin, Yuansheng Yang, Xiaoan Ruan, Ken Wing Kin Sung, Bernard Loo, Miranda Gek Sim Yap, Gavin Teo, Say Kong Ng, Wei Shou Hu, Pramila N. Ariyaratne, Gao Song, Dong-Yup Lee, Muriel Bardor, Ying Swan Ho, Shuwen Chen, Tessa Rui Min Tan, Niranjan Nagarajan, Hsueh Lee Lim, Meiyappan Lakshmanan
Publikováno v:
Cell Systems
Cell Systems, Elsevier, 2017, 4 (5), pp.530-542.e6. ⟨10.1016/j.cels.2017.04.009⟩
Cell Systems, Elsevier, 2017, 4 (5), pp.530-542.e6. ⟨10.1016/j.cels.2017.04.009⟩
Effective development of host cells for therapeutic protein production is hampered by the poor characterization of cellular transfection. Here, we employed a multi-omics-based systems biotechnology approach to elucidate the genotypic and phenotypic d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f78e4ba211751102ea86ce76d777c9f
https://doi.org/10.1016/j.cels.2017.04.009
https://doi.org/10.1016/j.cels.2017.04.009
Publikováno v:
Biotechnology progress. 25(1)
The presence of multiple copies of plasmids in Escherichia coli could induce a complex cascade of physiological changes known as the metabolic burden response. In this work, the physiological effect of such plasmid metabolic burden on E. coli metabol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b27661b888284d323ee5dde9983f9d44
https://pubmed.ncbi.nlm.nih.gov/19224555
https://pubmed.ncbi.nlm.nih.gov/19224555