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The effect of dietary protein level, water temperature and growth hormone administration on growth and metabolism in the common carp (Cyprinus carpio)

Autor: Arieh Gertler, Mira Fine, Zedi Cohen, Boaz Moav, Gad Degani, Dina Zilberg

Publikováno v: Comparative Biochemistry and Physiology Part A: Physiology. 114:35-42

Two experiments were carried out to study fish growth and several other metabolic parameters under three main effects: dietary protein level (40% vs. 21%); water temperature (26 vs. 17°C), and intraperitoneal administration of carp growth hormone (c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::978fd4ab84d680c79114e070c2fc9ac7
https://doi.org/10.1016/0300-9629(95)02085-3

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A short-time refolding procedure that dramatically increases the yields of recombinant monomeric carp growth hormone and its Cys123Ala mutant: Implications for other proteins with an unpaired number of cysteine residues

Autor: Arieh Gertler, Violet Daniel, Mira Fine, A. Levanon

Publikováno v: Biotechnology Techniques. 7:769-774

Recombinant carp growth hormone and its Cys123Ala analogue were refolded in 4.5 M urea, pH 11.3 in the presence of 0.1 mM cysteine. Shortening of the refolding process from 48 h to 1 h resulted in a 30 to 40 fold increase in yield of the biologically

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9b3680e2ba622702a4cdd0193bbe026d
https://doi.org/10.1007/bf00153742

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Preparation and comparison of biological properties of recombinant carp (Cyprinus carpio) growth hormone and its Cys-123 to Ala mutant

Autor: Nava Chapnik-Cohen, Orli Lipshitz, Avigdor Levanon, Mira Fine, Edna Sakal, Violet Daniel, Arieh Gertler, Dorit Vashdi

Publikováno v: Fish Physiology and Biochemistry. 11:353-361

Carp growth hormone (cGH) cDNA, in which Cys-123 was mutated to Ala, was prepared, transferred to the expression vector, expressed in Escherichia coli and the mutant was purified to homogeneity. The mutation only slightly improved yield of the monome

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f34f9bc5405b00e1f2354035faf78068
https://doi.org/10.1007/bf00004585

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Recombinant Carp (Cyprinus carpio) Growth Hormone: Expression, Purification, and Determination of Biological Activity in Vitro and in Vivo

Autor: Edna Sakal, Avigdor Levanon, Violet Daniel, Orli Lipshitz, Arieh Gertler, Dorit Vashdi, Mira Fine

Publikováno v: General and Comparative Endocrinology. 89:51-61

Carp growth hormone (cGH) cDNA (Koren et al., 1989) was cloned under the control of lambda-phage PLOL promoter and lambda cll ribosomal binding site into pBR322 plasmid to enable its expression in Escherichia coli A1645 that produces constitutively t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::088427423a0d6b988430f9d10f13737a
https://doi.org/10.1006/gcen.1993.1008

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Preparation of recombinant gilthead seabream (Sparus aurata) growth hormone and its use for stimulation of larvae growth by oral administration

Autor: A Tandler, Arieh Gertler, Benzion Cavari, S Maurice, Bruria Funkenstein, Mira Fine, I Ben-Atia

Publikováno v: General and comparative endocrinology. 113(1)

Gilthead seabream ( Sparus aurata ) growth hormone (gsGH) cDNA coding for the mature protein was cloned in a pGEM-T vector and then transferred into prokaryotic expression vector pET-8 and expressed in E. coli BL21 (DE3) cells upon induction with IPT

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1397ce82214e07316f238eb7c663658f
https://pubmed.ncbi.nlm.nih.gov/9882554

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