Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Ming-Kai Chern"'
Publikováno v:
Biocatalysis and Agricultural Biotechnology. 1:135-139
The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with desi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d72eeb9feadf8a7c54c5dc20f2046dc8
https://doi.org/10.1016/j.bcab.2011.09.004
https://doi.org/10.1016/j.bcab.2011.09.004
Publikováno v:
Fish Physiology and Biochemistry. 33:73-80
D-amino acid oxidase (DAO, EC 1.4.3.3) is one of the flavoenzymes in peroxisomes catalyzing the oxidation of D-amino acids to α-imino acids. By reverse transcription-polymerase chain reaction, DAO cDNA was cloned from the mRNA of zebrafish embryos.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::18e5a547f2731e5855da85673bf9cab7
https://doi.org/10.1007/s10695-006-9118-1
https://doi.org/10.1007/s10695-006-9118-1
Publikováno v:
Enzyme and Microbial Technology. 39:724-731
A protease-producing bacterium was isolated and identified as Bacillus sp. TKU004. It thus can be used for deproteinization of squid pen in the preparation of β-chitin. The optimized condition for protease production was found when the culture was s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f03f778410640738bd39f3423b1bccb2
https://doi.org/10.1016/j.enzmictec.2005.12.007
https://doi.org/10.1016/j.enzmictec.2005.12.007
Publikováno v:
Enzyme and Microbial Technology. 36:660-665
A fungus with protease and chitinase activities was isolated from the soil. It has been identified as Aspergillus fumigatus Fresenius TKU003. A. fumigatus TKU003 produced proteases and chitinases when it was grown in a medium containing shrimp and cr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91a4c42b1e70bf0305de361652aa2eab
https://doi.org/10.1016/j.enzmictec.2004.10.006
https://doi.org/10.1016/j.enzmictec.2004.10.006
Autor:
Kwang-Ning Chang, Tsuey-Chyi S. Tam, Yi-Lin Liang, Ming F. Tam, Ming-Kai Chern, Li-Fan Liu, Yi-Chen Liu
Publikováno v:
Journal of Biological Chemistry. 277:15345-15353
Type III protein-arginine methyltransferase from the yeast Saccharomyces cerevisiae (RMT2) was expressed in Escherichia coli and purified to apparent homogeneity. The cytosolic, ribosomal, and ribosome salt wash fractions from yeast cells lacking RMT
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58e5f517ab025c24b79d71b147019cbd
https://doi.org/10.1074/jbc.m111379200
https://doi.org/10.1074/jbc.m111379200
Publikováno v:
Analytical Biochemistry. 416:117-119
The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0193d29727eaf25f6a302b6ce340531
https://doi.org/10.1016/j.ab.2011.04.038
https://doi.org/10.1016/j.ab.2011.04.038
Autor:
Ming-Kai Chern, Regina Pietruszko
Publikováno v:
Biochemical and Biophysical Research Communications. 213:561-568
The E3 isozyme of human aldehyde dehydrogenase (EC 1.2.1.3), with broad substrate specificity, which also catalyzes dehydrogenation of 4-aminobutyraldehyde, was purified and sequenced recently (1,3). It has been shown during this investigation to hav
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8af8c4cb178968cf65d43da5e59270b
https://doi.org/10.1006/bbrc.1995.2168
https://doi.org/10.1006/bbrc.1995.2168
Autor:
Hui Ling Chen, Ming-Kai Chern, Chia-Ning Yang, Chun Yi Chang, Mei Yuan Hsu, Chung Wai Shiau, Cheng Wei Lo, Arthur Y. Shaw, Pei Jung Lu
Publikováno v:
European journal of medicinal chemistry. 45(7)
To continue our early study on the structural modifications of clioquinol, more 8-hydroxyquinoline-derived Mannich bases were synthesized and examined for growth-inhibitory effect. Taken Mannich base 1 as our lead compound, upon replacement of either
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cec71b0411fe76a891aebdd3fbf065db
https://pubmed.ncbi.nlm.nih.gov/20359788
https://pubmed.ncbi.nlm.nih.gov/20359788
Publikováno v:
Analytical biochemistry. 392(2)
Ion exchange chromatography, one of the major procedures for protein purification, seldom provides single-step purification due to a lack of specific affinity. In this work, a novel and simple method called "back flush" (i.e., reversing the flow dire
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf802b9f93b6d1856e83ff7f3c38a144
https://pubmed.ncbi.nlm.nih.gov/19497288
https://pubmed.ncbi.nlm.nih.gov/19497288
Publikováno v:
Biotechnology letters. 30(8)
D-amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis (RtDAO and TvDAO) are both yeast homodimeric flavoenzymes. Two of their cDNA genes were connected by a hexanucleotide linker and heterologously expressed in E. coli to pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2103d479325be5750bd3c5eda1f0c86
https://pubmed.ncbi.nlm.nih.gov/18330517
https://pubmed.ncbi.nlm.nih.gov/18330517