Zobrazeno 1 - 10
of 101
pro vyhledávání: '"Ming Qun Xu"'
Autor:
Yi Fang, Amogh Changavi, Manyun Yang, Luo Sun, Aihua Zhang, Daniel Sun, Zhiyi Sun, Boce Zhang, Ming‐Qun Xu
Publikováno v:
Advanced Science, Vol 9, Iss 3, Pp n/a-n/a (2022)
Abstract The requirement of a large input amount (500 ng) for Nanopore direct RNA‐seq presents a major challenge for low input transcriptomic analysis and early pathogen surveillance. The high RNA input requirement is attributed to significant samp
Externí odkaz:
https://doaj.org/article/c3549bf9ba7e4f9591d0530d02c18eb9
Autor:
Kiersten Flodman, Rebecca Tsai, Michael Y. Xu, Ivan R. Corrêa, Alyssa Copelas, Yan-Jiun Lee, Ming-Qun Xu, Peter Weigele, Shuang-yong Xu
Publikováno v:
Frontiers in Microbiology, Vol 10 (2019)
To counteract bacterial defense systems, bacteriophages (phages) make extensive base modifications (substitutions) to block endonuclease restriction. Here we evaluated Type II restriction of three thymidine (T or 5-methyldeoxyuridine, 5mdU) modified
Externí odkaz:
https://doaj.org/article/c061bb59c3ee467f8da1dfd0a0c1523d
Autor:
Inna Shcherbakova, Aaron A. Hoskins, Larry J. Friedman, Victor Serebrov, Ivan R. Corrêa, Jr., Ming-Qun Xu, Jeff Gelles, Melissa J. Moore
Publikováno v:
Cell Reports, Vol 5, Iss 1, Pp 151-165 (2013)
Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable
Externí odkaz:
https://doaj.org/article/523227fd46e342deafbe4a74dc36e217
Autor:
Ming-Qun Xu, Boce Zhang, Daniel Sun, Amogh Changavi, Manyun Yang, Aihua Zhang, Luo Sun, Zhiyi Sun, Yi Fang
Publikováno v:
Advanced Science, Vol 9, Iss 3, Pp n/a-n/a (2022)
Advanced Science
Advanced Science
The requirement of a large input amount (500 ng) for Nanopore direct RNA‐seq presents a major challenge for low input transcriptomic analysis and early pathogen surveillance. The high RNA input requirement is attributed to significant sample loss a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa157a430e6875e5f858501f83c204b5
https://doaj.org/article/c3549bf9ba7e4f9591d0530d02c18eb9
https://doaj.org/article/c3549bf9ba7e4f9591d0530d02c18eb9
Publikováno v:
BioTechniques, Vol 42, Iss 1, Pp 63-69 (2007)
Synthetic peptides incorporating various chemical moieties, for example, phosphate groups, are convenient tools for investigating protein modification enzymes, such as protein phosphatases (PPs). However, short peptides are sometimes poor substrates,
Externí odkaz:
https://doaj.org/article/faeb6fe5016a4a61a559ebd56c114e9e
Publikováno v:
BioTechniques, Vol 37, Iss 3, Pp 430-443 (2004)
Peptide arrays are increasingly used to define antibody epitopes and substrate specificities of protein kinases. Their use is hampered, however, by ineffective and variable binding efficiency of peptides, which often results in low sensitivity and in
Externí odkaz:
https://doaj.org/article/63a5416e9d9b451db09b4524f58f5aac
Publikováno v:
BioTechniques, Vol 36, Iss 6, Pp 976-981 (2004)
We have applied intein-mediated peptide ligation (IPL) to the use of peptide substrates for kinase assays and subsequent Western blot analysis. IPL allows for the efficient ligation of a synthetic peptide with an N-terminal cysteine residue to an int
Externí odkaz:
https://doaj.org/article/52c527ee533b46dca30614ea32b547bc
Publikováno v:
BioTechniques, Vol 27, Iss 1, Pp 110-120 (1999)
The Mycobacterium xenopi gyrase A mini-intein has been engineered to yield a controllable N-terminal or C-terminal, single- splice-junction autocleavage element. When combined with an affinity tag, these modified mini-inteins can be used to purify ta
Externí odkaz:
https://doaj.org/article/96bc59c360a040779f4b13eea8f3129a
Autor:
Benjamin A Smith, Shae B Padrick, Lynda K Doolittle, Karen Daugherty-Clarke, Ivan R Corrêa Jr, Ming-Qun Xu, Bruce L Goode, Michael K Rosen, Jeff Gelles
Publikováno v:
eLife, Vol 2 (2013)
During cell locomotion and endocytosis, membrane-tethered WASP proteins stimulate actin filament nucleation by the Arp2/3 complex. This process generates highly branched arrays of filaments that grow toward the membrane to which they are tethered, a
Externí odkaz:
https://doaj.org/article/101ba6f4735146f682e1059f11779d6f
Autor:
Ming-Qun Xu, Boce Zhang, Hayden Dillow, Manyun Yang, Daniel Sun, Jack Lepine, Luo Sun, Yaguang Luo, Xiaobo Liu, Alyssa Cousineau, Tingting Gu, Shaohua Li
Publikováno v:
Frontiers in Microbiology, Vol 11 (2020)
Frontiers in Microbiology
Frontiers in Microbiology
Viable pathogenic bacteria are major biohazards that pose a significant threat to food safety. Despite the recent developments in detection platforms, multiplex identification of viable pathogens in food remains a major challenge. A novel strategy is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e5f3fc0a2914ba74dfbe1603b5a3618
https://www.frontiersin.org/article/10.3389/fmicb.2020.00514/full
https://www.frontiersin.org/article/10.3389/fmicb.2020.00514/full