Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered Saccharomyces cerevisiae.

Autor: Jian Zha, Bing-Zhi Li, Ming-Hua Shen, Meng-Long Hu, Hao Song, Ying-Jin Yuan

Publikováno v: PLoS ONE, Vol 8, Iss 7, p e68317 (2013)

Production of ethanol and xylitol from lignocellulosic hydrolysates is an alternative to the traditional production of ethanol in utilizing biomass. However, the conversion efficiency of xylose to xylitol is restricted by glucose repression, causing

Externí odkaz: https://doaj.org/article/5857ab96df7e4c1cb9613e316667a61a

'Perfect' designer chromosome V and behavior of a ring derivative

Autor: Ning Zhi Liu, Wen Zheng Zhang, Qiu Hui Lin, Ting Liu, Jing Sheng Cheng, Mei Qing Fu, Kun Yang, Ming Zhu Ding, Ran Tao, J. Andrew Martin, Yue Shen, Xia Li, Jef D. Boeke, Guo Zhen Jiang, Zhi Chao Yu, Si Chen, Wan Su, Jin Gui Liu, Jian Jun Qiao, Yan Wang, Shi Yang Liu, Wen Qian Zhang, Su Wang, Hang Xu, Shi Lei Han, Wei Liu, Ken Chen, Yi Wu, Yue Liu, Ze-Xiong Xie, Juan Zhao, Yi Lin Liu, Roy Walker, Li Xiang Song, Ye Xuan Deng, Xuya Wang, Xia Wang, Rui Guo, Leslie A. Mitchell, Joel S. Bader, Ting Ting Zhang, Ming Hua Shen, Guang Rong Zhao, Xiao Tong Wei, Xiao Ran Xu, Bing-Zhi Li, Jun Qi Zhu, Ying-Jin Yuan, Hao Xing Du, Bo Xuan Zeng, Yi Ran Wang, Bin Jia, Zheng Kuang, Shi Lan Yang, Ting Li, Lan Meng Qu, Jia Qing Zhu, Kai Ren Tian, Ping Sheng Ma, Tian Qing Song, Xue Nan Li, Guang Xin Ye, Cheng Hu, Huanming Yang, Jia Fei Lv, Wei Zhang, Yisha Luo, Qi Feng, Zhu Jin, Zhen Ning Liu, Wen Qi Ding, Fang Zhai, Xin Qi, Jin Hua Zhang, Xiao Le Wu, Yizhi Cai, Meng Zhao, Xue Jiao Guo, Xue Bai, Jun Jun Dai, Meng Long Hu, Fei Fei Li, Si Yu Xin, Xiao Na Yang, En Xu Wang, Giovanni Stracquadanio, Hui Min Liu, Lin Ting Wang, Chun-Ting Zhang, Zheng Bao Xia, Da Shuai Li, Yun Wang, Nan Jia

Publikováno v: Xie, Z-X, Li, B-Z, Mitchell, L A, Wu, Y, Qi, X, Jin, Z, Jia, B, Wang, X, Zeng, B-X, Liu, H-M, Wu, X-L, Feng, Q, Zhang, W-Q, Liu, W, Ding, M-Z, Li, X, Zhao, G-R, Qiao, J-J, Cheng, J-S, Zhao, M, Kuang, Z, Wang, X, Martin, J A, Stracquadanio, G, Yang, K, Bai, X, Zhao, J, Hu, M-L, Lin, Q-H, Zhang, W-Q, Shen, M-H, Chen, S, Su, W, Wang, E-X, Guo, R, Zhai, F, Guo, X-J, Du, H-X, Zhu, J-Q, Song, T-Q, Dai, J-J, Li, F-F, Jiang, G-Z, Han, S-L, Liu, S-Y, Yu, Z-C, Yang, X-N, Chen, K, Hu, C, Li, D-S, Jia, N, Liu, Y, Wang, L-T, Wang, S, Wei, X-T, Fu, M-Q, Qu, L-M, Xin, S-Y, Liu, T, Tian, K-R, Li, X-N, Zhang, J-H, Song, L-X, Liu, J-G, Lv, J-F, Xu, H, Tao, R, Wang, Y, Zhang, T-T, Deng, Y-X, Wang, Y-R, Li, T, Ye, G-X, Xu, X-R, Xia, Z-B, Zhang, W, Yang, S-L, Liu, Y-L, Ding, W-Q, Liu, Z-N, Zhu, J-Q, Liu, N-Z, Walker, R, Luo, Y, Wang, Y, Shen, Y, Yang, H, Cai, Y, Ma, P-S, Zhang, C-T, Bader, J S, Boeke, J D & Yuan, Y-J 2017, ' "Perfect" designer chromosome V and behavior of a ring derivative ', Science, vol. 355, no. 6329 . https://doi.org/10.1126/science.aaf4704

INTRODUCTION The Saccharomyces cerevisiae 2.0 project (Sc2.0) aims to modify the yeast genome with a series of densely spaced designer changes. Both a synthetic yeast chromosome arm (synIXR) and the entirely synthetic chromosome (synIII) function wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e38923b67576ca15f20973c8d990e9b6
https://doi.org/10.1126/science.aaf4704

Deletion of D-ribulose-5-phosphate 3-epimerase (RPE1) induces simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae

Autor: Ming-Hua Shen, Hao Song, Ying-Jin Yuan, Bing-Zhi Li

Publikováno v: Biotechnology letters. 37(5)

Simultaneous co-utilization of xylose and glucose is a key issue in engineering microbes for cellulosic ethanol production. We coupled xylose utilization with glucose metabolism by deletion of D-ribulose-5-phosphate 3-epimerase (RPE1) through pentose

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2df0f133ca6453ea1fba9202dc7f7f00
https://pubmed.ncbi.nlm.nih.gov/25548118

Enhanced expression of genes involved in initial xylose metabolism and the oxidative pentose phosphate pathway in the improved xylose-utilizing Saccharomyces cerevisiae through evolutionary engineering

Autor: Ming-Hua Shen, Jian Zha, Hao Song, Ying-Jin Yuan, Hu Menglong

Publikováno v: Journal of industrial microbiologybiotechnology. 41(1)

Fermentation of xylose in lignocellulosic hydrolysates by Saccharomyces cerevisiae has been achieved through heterologous expression of the xylose reductase (XR)–xylitol dehydrogenase (XDH) pathway. However, the fermentation efficiency is far from

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b447d234fbb46f29ace1cd1af1da096
https://pubmed.ncbi.nlm.nih.gov/24113893

Purification, characterization, and cloning of fibrinolytic metalloprotease from Pleurotus ostreatus mycelia

Autor: Ming-Hua, Shen, Jae-Sung, Kim, Kumar, Sapkota, Se-Eun, Park, Bong-Suk, Choi, Seung, Kim, Hyun-Hwa, Lee, Chun-Sung, Kim, Hong-Sung, Chun, Cheon-In, Ryoo, Sung-Jun, Kim

Publikováno v: Journal of microbiology and biotechnology. 17(8)

A fibrinolytic protease (PoFE) was purified from the cultured mycelia of the edible oyster mushroom Pleurotus ostreatus, using a combination of various chromatographies. The purification protocol resulted in an 876-fold purification of the enzyme, wi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::097b87ac580f174a1392754225526ae4
https://pubmed.ncbi.nlm.nih.gov/18051595