Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Milos A. Cvetkovic"'
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103237- (2024)
Summary: Here, we present a large-scale FLAG immunoprecipitation protocol to isolate large protein complexes driving DNA replication at replicating chromatin assembled in Xenopus laevis egg extract. We describe how to prepare demembranated sperm nucl
Externí odkaz:
https://doaj.org/article/d3a3b49bd9604fb0a3137a6c26adea9e
Pol epsilon is a tetrameric assembly that plays distinct roles during eukaryotic chromosome replication. It catalyses leading strand DNA synthesis; yet this function is dispensable for viability. Its non-catalytic domains instead play an essential ro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7641ac9ed3bac23e0c048b125c9499e9
Autor:
Tanja Consolati, Julia Locke, Juri Rappsilber, Johanna Roostalu, Zhuo A. Chen, Jayant Asthana, Thomas Surrey, Wei Ming Lim, Fabrizio Martino, Alessandro Costa, Julian Gannon, Milos A. Cvetkovic
Publikováno v:
Developmental Cell
Consolati, T, Locke, J, Roostalu, J, Chen, Z A, Gannon, J, Asthana, J, Lim, W M, Martino, F, Cvetkovic, M A, Rappsilber, J, Costa, A & Surrey, T 2020, ' Microtubule nucleation properties of single human γTuRCs explained by their Cryo-EM structure ', Developmental Cell, vol. 53, no. 5, pp. 603-617.e8 . https://doi.org/10.1016/j.devcel.2020.04.019
Consolati, T, Locke, J, Roostalu, J, Chen, Z A, Gannon, J, Asthana, J, Lim, W M, Martino, F, Cvetkovic, M A, Rappsilber, J, Costa, A & Surrey, T 2020, ' Microtubule nucleation properties of single human γTuRCs explained by their Cryo-EM structure ', Developmental Cell, vol. 53, no. 5, pp. 603-617.e8 . https://doi.org/10.1016/j.devcel.2020.04.019
Summary The γ-tubulin ring complex (γTuRC) is the major microtubule nucleator in cells. The mechanism of its regulation is not understood. We purified human γTuRC and measured its nucleation properties in a total internal reflection fluorescence (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11d6723b0e714c9df2a7949e6a1db9b2
Publikováno v:
Nature chemical biology
The exosome is a large molecular machine that is involved in RNA degradation and processing. Here, we address how the trimeric Rrp4 cap enhances the activity of the archaeal enzyme complex. Using methyl TROSY NMR methods we identified a 50 Å long RN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bd81b0727764d2bd8ed276eaca4bd33
https://doi.org/10.1038/nchembio.2328
https://doi.org/10.1038/nchembio.2328
Autor:
Wei Ming Lim, Julia Locke, Jayant Asthana, Johanna Roostalu, Fabrizio Martino, Milos A. Cvetkovic, Juri Rappsilber, Julian Gannon, Zhuo Angel Chen, Thomas Surrey, Alessandro Costa, Tanja Consolati
Publikováno v:
Biophysical Journal. 120:106a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ab8b6bb9f3e3e1be6037b830fb825a22
https://doi.org/10.1016/j.bpj.2020.11.855
https://doi.org/10.1016/j.bpj.2020.11.855
Publikováno v:
Nucleic Acids Research
The exosome plays an important role in RNA degradation and processing. In archaea, three Rrp41:Rrp42 heterodimers assemble into a barrel like structure that contains a narrow RNA entrance pore and a lumen that contains three active sites. Here, we de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47b6379e4669b04a185c5a38abfb3de3
https://doi.org/10.1093/nar/gkw062
https://doi.org/10.1093/nar/gkw062