Nuclear eIF4E Stimulates 3′-End Cleavage of Target RNAs

Autor: Margaret Rose Davis, Mildred Delaleau, Katherine L.B. Borden

Publikováno v: Cell Reports, Vol 27, Iss 5, Pp 1397-1408.e4 (2019)

Summary: The eukaryotic translation initiation factor eIF4E is nuclear and cytoplasmic where it plays roles in export and translation of specific transcripts, respectively. When we were studying its mRNA export activity, we unexpectedly discovered th

Externí odkaz: https://doaj.org/article/48357669f435479387bb7fc6d5bf602e

A scalable framework for the discovery of functional helicase substrates and helicase-driven regulatory switches

Autor: Mildred Delaleau, Eric Eveno, Isabelle Simon, Annie Schwartz, Marc Boudvillain

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (38), ⟨10.1073/pnas.2209608119⟩

Helicases are ubiquitous motor enzymes that remodel nucleic acids (NA) and NA–protein complexes in key cellular processes. To explore the functional repertoire and specificity landscape of helicases, we devised a screening scheme—Helicase-SELEX (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73ec7310981b49c1b1db95c0bc7a2388
https://doi.org/10.1073/pnas.2209608119

A large insertion domain in the Rho factor from a low G+C, Gram-negative bacterium is critical for RNA binding and transcription termination activity

Autor: Mildred Delaleau, Marc Boudvillain, Isabelle Simon, Annie Schwartz

Publikováno v: Journal of Molecular Biology
Journal of Molecular Biology, 2021, 433 (15), pp.167060. ⟨10.1016/j.jmb.2021.167060⟩
Journal of Molecular Biology, Elsevier, 2021, 433 (15), pp.167060. ⟨10.1016/j.jmb.2021.167060⟩

International audience; Rho-dependent termination of transcription (RDTT) is a critical regulatory mechanism specific to bacteria. In a subset of species including most Actinobacteria and Bacteroidetes, the Rho factor contains a large, poorly conserv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be9a3bfb26647f14ac0f28a49d4ccd12
https://cnrs.hal.science/hal-03248336

Monitoring eIF4E-Dependent Nuclear 3' End mRNA Cleavage

Autor: Mildred, Delaleau

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2209

In eukaryotes, the maturation of the 3' ends of most transcripts involves cleavage and polyadenylation steps in the nucleus. While I was working in the group of Katherine Borden at the University of Montréal, we reported that the eukaryotic translat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::84e8ee833301b49f4e6eaa581c93cc0c
https://pubmed.ncbi.nlm.nih.gov/33201480

Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro

Autor: Mildred Delaleau, Cédric Nadiras, Annie Schwartz, Marc Boudvillain

Publikováno v: Methods in Mecular Biology
Methods in Mecular Biology, 1737, humana press, Totowa, NJ-USA, pp.99-118, 2018, Bacterial regulatory RNA (Arluison & Valverde, eds), ⟨10.1007/978-1-4939-7634-8_7⟩
Methods in Molecular Biology ISBN: 9781493976331

International audience; Besides their well-known posttranscriptional effects on mRNA translation and decay, sRNAs and associated RNA chaperones (e.g., Hfq, CsrA) sometimes regulate gene expression at the transcriptional level. In this case, the sRNA-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0c167664056ef1d21e7b5a40ff38b35
https://hal.archives-ouvertes.fr/hal-01880631