A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus.

Autor: Simon Kelly, Simon B Hansen, Henriette Rübsam, Pia Saake, Emil B Pedersen, Kira Gysel, Eva Madland, Shunliang Wu, Stephan Wawra, Dugald Reid, John T Sullivan, Zuzana Blahovska, Maria Vinther, Artur Muszynski, Parastoo Azadi, Mikkel B Thygesen, Finn L Aachmann, Clive W Ronson, Alga Zuccaro, Kasper R Andersen, Simona Radutoiu, Jens Stougaard

Publikováno v: PLoS Biology, Vol 21, Iss 5, p e3002127 (2023)

Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely

Externí odkaz: https://doaj.org/article/2791f09a8f9a4452873882f085b3113f

Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence

Autor: Manuel C. Martos-Maldonado, Christian T. Hjuler, Kasper K. Sørensen, Mikkel B. Thygesen, Jakob E. Rasmussen, Klaus Villadsen, Søren R. Midtgaard, Stefan Kol, Sanne Schoffelen, Knud J. Jensen

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)

His-tagged proteins can undergo N-terminal acylation as an undesired side-reaction. Here, the authors utilize this to develop a method for highly selective acylation and further modification of peptides and proteins using an optimized His sequence an

Externí odkaz: https://doaj.org/article/3e78d4a3733a4cc3b314ad992f024827

A plant chitinase controls cortical infection thread progression and nitrogen-fixing symbiosis

Autor: Anna Malolepszy, Simon Kelly, Kasper Kildegaard Sørensen, Euan Kevin James, Christina Kalisch, Zoltan Bozsoki, Michael Panting, Stig U Andersen, Shusei Sato, Ke Tao, Dorthe Bødker Jensen, Maria Vinther, Noor de Jong, Lene Heegaard Madsen, Yosuke Umehara, Kira Gysel, Mette U Berentsen, Mickael Blaise, Knud Jørgen Jensen, Mikkel B Thygesen, Niels Sandal, Kasper Røjkjær Andersen, Simona Radutoiu

Publikováno v: eLife, Vol 7 (2018)

Morphogens provide positional information and their concentration is key to the organized development of multicellular organisms. Nitrogen-fixing root nodules are unique organs induced by Nod factor-producing bacteria. Localized production of Nod fac

Externí odkaz: https://doaj.org/article/b952e901cfbb4086acadeb7473113fef

Highly Selective Lysine Acylation in Proteins Using a Lys-His Tag Sequence

Autor: Christian Kofoed, Shunliang Wu, Kasper K. Sørensen, Tuule Treiberg, Johnny Arnsdorf, Sara P. Bjørn, Tanja L. Jensen, Bjørn G. Voldborg, Mikkel B. Thygesen, Knud J. Jensen, Sanne Schoffelen

Publikováno v: Kofoed, C, Wu, S, Sørensen, K K, Treiberg, T, Arnsdorf, J, Bjørn, S P, Jensen, T L, Voldborg, B G, Thygesen, M B, Jensen, K J & Schoffelen, S 2022, ' Highly Selective Lysine Acylation in Proteins Using a Lys-His Tag Sequence ', Chemistry: A European Journal, vol. 28, no. 15, e202200147 . https://doi.org/10.1002/chem.202200147
Kofoed, C, Wu, S, Sørensen, K K, Treiberg, T, Arnsdorf, J, Bjørn, S P, Jensen, T L, Voldborg, B G, Thygesen, M B, Jensen, K J & Schoffelen, S 2022, ' Highly selective lysine acylation in proteins using a Lys-His tag sequence ', Chemistry-A European Journal, vol. 28, no. 15 . https://doi.org/10.1002/chem.202200147

Chemical modification of proteins has numerous applications, but it has been challenging to achieve the required high degree of selectivity on lysine amino groups. Recently, we described the highly selective acylation of proteins with an N-terminal G

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51595d1281f109d67226de44c10a40e4
https://curis.ku.dk/portal/da/publications/highly-selective-lysine-acylation-in-proteins-using-a-lyshis-tag-sequence(e5726e1f-758b-4b67-9a4d-0598a064138f).html

Improved Characterization of Nod Factors and Genetically Based Variation in LysM Receptor Domains Identify Amino Acids Expendable for Nod Factor Recognition in Lotus spp.

Autor: Anita S. Bek, Jørgen Sauer, Mikkel B. Thygesen, Jens Ø. Duus, Bent O. Petersen, Søren Thirup, Euan James, Knud J. Jensen, Jens Stougaard, Simona Radutoiu

Publikováno v: Molecular Plant-Microbe Interactions, Vol 23, Iss 1, Pp 58-66 (2010)

Formation of functional nodules is a complex process depending on host–microsymbiont compatibility in all developmental stages. This report uses the contrasting symbiotic phenotypes of Lotus japonicus and L. pedunculatus, inoculated with Mesorhizob

Externí odkaz: https://doaj.org/article/c2cc6f9827904d138834f28a1bebd3cf