Regulation of hepatocyte cell cycle re-entry by RNA polymerase II-associated Gdown1

Autor: Miki Jishage, Robert G. Roeder

Publikováno v: Cell Cycle

Liver is the central organ responsible for whole-body metabolism, and its constituent hepatocytes are the major players that carry out liver functions. Although they are highly differentiated and rarely divide, hepatocytes re-enter the cell cycle fol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd857c4d72b519c29f73db7eaefdaa5f
https://doi.org/10.1080/15384101.2020.1843776

Architecture of Pol II(G) and molecular mechanism of transcription regulation by Gdown1

Autor: Robert G. Roeder, Sai J. Ganesan, Miki Jishage, Xiaodi Yu, Andrej Sali, Yi Shi, Brian T. Chait, Wei Yi Chen, Francisco J. Asturias

Publikováno v: Nature structural & molecular biology
Nature structural & molecular biology, vol 25, iss 9
Nature Structural and Molecular Biology, vol 25, iss 9

Tight binding of Gdown1 represses RNA polymerase II (Pol II) function in a manner that is reversed by Mediator, but the structural basis of these processes is unclear. Although Gdown1 is intrinsically disordered, its Pol II interacting domains were l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1861467b409dbe786053fc2b400a085d
https://doi.org/10.1038/s41594-018-0118-5

Transcriptional Regulation by Pol II(G) Involving Mediator and Competitive Interactions of Gdown1 and TFIIF with Pol II

Autor: Beatrix Uberheide, Ulrich Wagner, Miki Jishage, Averell Gnatt, Sohail Malik, Yasushi Ishihama, Xiaopeng Hu, Brian T. Chait, Robert G. Roeder, Bing Ren

Publikováno v: Molecular Cell. 45:51-63

Pol II(G) is a distinct form of RNA polymerase II that contains the tightly associated Gdown1 polypeptide (encoded by POLR2M). Unlike Pol II, Pol II(G) is highly dependent upon Mediator for robust activator-dependent transcription in a biochemically

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d761a8343f6b0682c255c8b6ea2ca6d
https://doi.org/10.1016/j.molcel.2011.12.014

Mapping of the Rsd Contact Site on the Sigma 70 Subunit of Escherichia coli RNA Polymerase

Autor: Akira Ishihama, Dipak Dasgupta, Miki Jishage

Publikováno v: Journal of Bacteriology. 183:2952-2956

Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase ς 70 subunit. The contact site of Rsd on ς 70 was analyzed after mapping of the contact-dependent cleavage sites by Rsd-tethered iron- p -bromoacetamidobenzy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46f774ee054cc1f3420d919f33509cdc
https://doi.org/10.1128/jb.183.9.2952-2956.2001

Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of four species of sigma subunit under various growth conditions

Autor: Akira Ishihama, S Ueda, A Iwata, Miki Jishage

Publikováno v: Journal of Bacteriology. 178:5447-5451

By a quantitative Western immunoblot analysis, the intracellular levels of two principal sigma subunits, sigma 70 (sigma D, the rpoD gene product) and sigma 38 (sigma S, the rpoS gene product), and of two minor sigma subunits, sigma 54 (sigma N, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75e25719d5e71e1722479e9e83b31554
https://doi.org/10.1128/jb.178.18.5447-5451.1996