Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Mika Maenaka"'
Autor:
Yoshihiro Ohmiya, Kazuki Niwa, Masahiro Takeo, Dai-ichiro Kato, Seiji Negoro, Takaya Kubo, Mika Maenaka
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 87:18-23
The bioluminescence color of firefly luciferase including its mutants ranges from green to red (530–640 nm) and is affected by the species of firefly, the reaction conditions, and by the substitution of amino acids. Although there is a general agre
Autor:
Seiji Negoro, Daiki Shirakawa, Kazuki Niwa, Robin Polz, Masahiro Takeo, Mika Maenaka, Dai-ichiro Kato
Publikováno v:
Photochemicalphotobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology. 13(12)
A simple reaction procedure for chemiluminescence of firefly luciferin (D-luc) using n-propylphosphonic anhydride (T3P) is reported. A luminescent photon is produced as a result of one-pot reaction, only requiring mixing with the substrate carboxylic
Autor:
Yoshihiro Ohmiya, Masahiro Takeo, Kazuki Niwa, Seiji Negoro, Yoshihiro Hiraishi, Mika Maenaka, Keisuke Yokoyama, Dai-ichiro Kato
Publikováno v:
Journal of biotechnology. 168(3)
We identified the critical amino acid residues for substrate recognition using two firefly luciferases from Pylocoeria miyako (PmL) and Hotaria parvura (HpL), as these two luciferase enzymes exhibit different activities toward ketoprofen. Specificall