Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Miho Terashima"'
Publikováno v:
Uirusu. 57:37-45
The adenovirus vector is very attractive tool not only for the gene therapy but also for the basic sciences. However, because a construction method of this vector had been complex, only limited scientists had constructed and enjoyed the benefits. Rec
Publikováno v:
Microbiology and Immunology. 50:643-654
The entire cloned human adenovirus type 5 (Ad5) genome is known to be able to generate infectious virus after transfection into 293 cells when the both ends of the genome are exposed by digestion with appropriate restriction enzymes. However, when on
Autor:
Takehide Murata, Bingbing Liu, Hatsumi Nakata, Jianzhi Pan, Megumi Hirose, Izumu Saito, Sanae Inamoto, Hideyo Ugai, Kazunari K. Yokoyama, Yukari Kujime, Koji Nakade, Erika Suzuki, Makoto Kimura, Yoshinori Nagamura, Kumiko Inabe, Yoshihiro Ugawa, Takahito Yamasaki, Hirofumi Hamada, Yuichi Obata, Miho Terashima
Publikováno v:
The Journal of Gene Medicine. 7:1148-1157
Background Viral vectors are required as gene-delivery systems for gene therapy and basic research. Recombinant adenoviruses (rAds) expressing genes of interest are being developed as research tools and many studies in vitro and in vivo have already
Autor:
Hong Tang, Yuichi Obata, Miho Terashima, Hideyo Ugai, Makoto Kimura, Takehide Murata, Jianzhi Pan, Kazunari K. Yokoyama, Megumi Hirose, Yukari Kujime, Bingbing Liu, Mujun Zhao, Kumiko Inabe, Takahito Yamasaki, Hirofumi Hamada
Publikováno v:
Biochemical and Biophysical Research Communications. 331:1053-1060
Adenoviruses are excellent vectors for gene transfer and are used extensively for high-level expression of the products of transgenes in living cells. The development of simple and rapid methods for the purification of stable infectious recombinant a
Autor:
Zheng Pei, Saki Kondo, Miho Terashima, Hiromitsu Fukuda, Izumu Saito, Yumi Kanegae, Aya Maekawa
Publikováno v:
Nucleic Acids Research
Tissue-/cancer-specific promoters for use in adenovirus vectors (AdVs) are valuable for elucidating specific gene functions and for use in gene therapy. However, low activity, non-specific expression and size limitations in the vector are always prob
Autor:
Kazunari K. Yokoyama, Takehide Murata, Yukari Kujime, Kumiko Inabe, Sanae Inamoto, Megumi Hirose, Erika Suzuki, Takahito Yamasaki, Miho Terashima, Hideyo Ugai
Publikováno v:
Science. 307:1722-1722
Viral vectors have been developed as therapeutic agents for the introduction of exogenous genes into living cells ([1][1]), and clinical trials of gene therapy and the use of viral vectors in the laboratory have been reported with increasing frequenc
Autor:
Naoto Tachikawa, Miho Terashima, Izumu Saito, Saki Kondo, Hiromi Sato, Aya Okuda, Yumi Kanegae
Publikováno v:
Nucleic Acids Research. 31:76e-76
The site-specific recombinase Cre has often been used for on/off regulation of expression of transgenes introduced into the mammalian chromosome. However, this method is only applicable to the regulation of a single gene and cannot be used to simulta