High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistance

Autor: Mariela Cortés-López, Laura Schulz, Mihaela Enculescu, Claudia Paret, Bea Spiekermann, Mathieu Quesnel-Vallières, Manuel Torres-Diz, Sebastian Unic, Anke Busch, Anna Orekhova, Monika Kuban, Mikhail Mesitov, Miriam M. Mulorz, Rawan Shraim, Fridolin Kielisch, Jörg Faber, Yoseph Barash, Andrei Thomas-Tikhonenko, Kathi Zarnack, Stefan Legewie, Julian König

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)

Multiple alternative splicing events in CD19 mRNA have been associated with resistance/relapse to CD19 CAR-T therapy in patients with B cell malignancies. Here, by combining patient data and a high-throughput mutagenesis screen, the authors identify

Externí odkaz: https://doaj.org/article/c08d347a8dc943b8ab905da6ad76b36d

Modelling Systemic Iron Regulation during Dietary Iron Overload and Acute Inflammation: Role of Hepcidin-Independent Mechanisms.

Autor: Mihaela Enculescu, Christoph Metzendorf, Richard Sparla, Maximilian Hahnel, Johannes Bode, Martina U Muckenthaler, Stefan Legewie

Publikováno v: PLoS Computational Biology, Vol 13, Iss 1, p e1005322 (2017)

Systemic iron levels must be maintained in physiological concentrations to prevent diseases associated with iron deficiency or iron overload. A key role in this process plays ferroportin, the only known mammalian transmembrane iron exporter, which re

Externí odkaz: https://doaj.org/article/9cbcb652b269424cad432e7491897bbd

High-throughput mutagenesis identifies mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistance

Autor: Stefan Legewie, Kathi Zarnack, Claudia Paret, Mariela Cortés-López, Jörg Faber, Fridolin Kielisch, Mathieu Quesnel-Vallières, Anna Orekhova, Andrei Thomas-Tikhonenko, Anke Busch, Mihaela Enculescu, Julian König, Laura Schulz, Bea Spiekermann, Manuel Torres-Diz, Yoseph Barash

During CART-19 immunotherapy for B-cell acute lymphoblastic leukaemia (B-ALL), many patients relapse due to loss of the cognate CD19 epitope. Since epitope loss can be caused by aberrant CD19 exon 2 processing, we herein investigate the regulatory co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::78ef49a673da74a4df65fb6f1b26d7d4
https://doi.org/10.1101/2021.10.08.463671

Exon definition facilitates reliable control of alternative splicing in the RON proto-oncogene

Autor: Stefan Legewie, Julian Koenig, Simon Braun, Kathi Zarnack, Samarth Thonta Setty, Mihaela Enculescu

Alternative splicing is a key step in eukaryotic gene expression that allows the production of multiple protein isoforms from the same gene. Even though splicing is perturbed in many diseases, we currently lack insights into regulatory mechanisms pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51ba05c4a0e39d111b00a2ac1ade06c7
https://doi.org/10.1101/714022

Decoding a cancer-relevant splicing decision in the RON proto-oncogene using high-throughput mutagenesis

Autor: F. X. Reymond Sutandy, Samarth Thonta Setty, Mihaela Enculescu, Stefan Legewie, Stefanie Ebersberger, Laura Schulz, Anke Busch, Julian König, Kathi Zarnack, Mariela Cortés-López, Simon Braun, Bernardo P. de Almeida, Markus Seiler, Nuno L. Barbosa-Morais

Publikováno v: Nature Communications
Nature Communications, Vol 9, Iss 1, Pp 1-18 (2018)
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5bffdea532902f0ddb7844dd8fbc1d9
https://hdl.handle.net/10451/50039