β2 integrins impose a mechanical checkpoint on macrophage phagocytosis

Autor: Alexander H. Settle, Benjamin Y. Winer, Miguel M. de Jesus, Lauren Seeman, Zhaoquan Wang, Eric Chan, Yevgeniy Romin, Zhuoning Li, Matthew M. Miele, Ronald C. Hendrickson, Daan Vorselen, Justin S. A. Perry, Morgan Huse

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-20 (2024)

Abstract Phagocytosis is an intensely physical process that depends on the mechanical properties of both the phagocytic cell and its chosen target. Here, we employed differentially deformable hydrogel microparticles to examine the role of cargo rigid

Externí odkaz: https://doaj.org/article/7bde948fab0947f0a3ebd774da92a46f

Autologous Adipose-Derived Mesenchymal Stromal Cells for the Treatment of Psoriasis Vulgaris and Psoriatic Arthritis: A Case Report

Autor: Miguel M. De Jesus, Jayson S. Santiago, Camille V. Trinidad, Melvin E. See, Kimberly R. Semon, Manuel O. Fernandez, Francisco S. Chung Ph.D.

Publikováno v: Cell Transplantation, Vol 25 (2016)

Psoriasis is a dermatologic disease of immune origins with no definitive cure. We report the Makati Medical Center experience of utilizing autologous mesenchymal stromal cells (MSCs) for one patient with psoriasis vulgaris (PV) and another with psori

Externí odkaz: https://doaj.org/article/ff8a1dcf703a410eb5a123d04435f066

Single-cell topographical profiling of the immune synapse reveals a biomechanical signature of cytotoxicity.

Autor: de Jesus M; Louis V. Gerstner, Jr., Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Settle AH; Louis V. Gerstner, Jr., Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Vorselen D; Cell Biology and Immunology Group, Wageningen University & Research, Wageningen, Netherlands.; Department of Biology, University of Washington, Seattle, WA, USA., Gaetjens TK; Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA., Galiano M; Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Romin Y; Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Lee E; Immunology & Molecular Pathogenesis Program, Weill Cornell Medicine Graduate School of Medical Sciences, New York, NY, USA., Wong YY; Louis V. Gerstner, Jr., Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Fu TM; Department of Electrical and Computer Engineering, Princeton University, Princeton, NJ, USA., Santosa E; Immunology & Molecular Pathogenesis Program, Weill Cornell Medicine Graduate School of Medical Sciences, New York, NY, USA., Winer BY; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Tamzalit F; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Wang MS; Pharmacology Program, Weill Cornell Medicine Graduate School of Medical Sciences, New York, NY, USA., Santella A; Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Bao Z; Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Sun JC; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Shah P; Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA., Theriot JA; Department of Biology, University of Washington, Seattle, WA, USA., Abel SM; Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA., Huse M; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Publikováno v: Science immunology [Sci Immunol] 2024 Jun 28; Vol. 9 (96), pp. eadj2898. Date of Electronic Publication: 2024 Jun 28.

Apoptotic contraction drives target cell release by cytotoxic T cells.

Autor: Sanchez EE; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Tello-Lafoz M; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Guo AJ; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., de Jesus M; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Louis V. Gerstner Jr Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Elbanna YA; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Louis V. Gerstner Jr Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Winer BY; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Budhu S; Department of Pharmacology, Weill-Cornell Medical College, New York, NY, USA., Chan E; Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Rosiek E; Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Kondo T; Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA., DuSold J; Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA., Taylor N; Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA., Altan-Bonnet G; Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA., Olson MF; Department of Chemistry and Biology, Toronto Metropolitan University, Toronto, Ontario, Canada., Huse M; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA. husem@mskcc.org.

Publikováno v: Nature immunology [Nat Immunol] 2023 Sep; Vol. 24 (9), pp. 1434-1442. Date of Electronic Publication: 2023 Jul 27.

Mechanically active integrins target lytic secretion at the immune synapse to facilitate cellular cytotoxicity.

Autor: Wang MS; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Pharmacology Program, Weill-Cornell Graduate School of Medical Sciences, New York, NY, USA., Hu Y; Department of Chemistry, Emory University, Atlanta, GA, USA., Sanchez EE; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Biochemistry and Molecular Biology Program, Weill-Cornell Graduate School of Medical Sciences, New York, NY, USA., Xie X; Neuroscience Program, Weill-Cornell Graduate School of Medical Sciences, New York, NY, USA., Roy NH; Department of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, NY, USA., de Jesus M; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Winer BY; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Zale EA; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Jin W; Department of Biomedical Engineering, Columbia University, New York, NY, USA., Sachar C; Department of Biomedical Engineering, Columbia University, New York, NY, USA., Lee JH; Department of Biomedical Engineering, Columbia University, New York, NY, USA., Hong Y; Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA., Kim M; Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA., Kam LC; Department of Biomedical Engineering, Columbia University, New York, NY, USA., Salaita K; Department of Chemistry, Emory University, Atlanta, GA, USA., Huse M; Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA. husem@mskcc.org.

Publikováno v: Nature communications [Nat Commun] 2022 Jun 09; Vol. 13 (1), pp. 3222. Date of Electronic Publication: 2022 Jun 09.