Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Autor: Javier Garrigós-Martínez, Kiira Vuoristo, Miguel Angel Nieto-Taype, Juha Tähtiharju, Jaana Uusitalo, Pauliina Tukiainen, Christian Schmid, Ilya Tolstorukov, Knut Madden, Merja Penttilä, José Luis Montesinos-Seguí, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega

Publikováno v: Microbial Cell Factories, Vol 20, Iss 1, Pp 1-12 (2021)

Abstract Background Pichia pastoris is a powerful and broadly used host for recombinant protein production (RPP), where past bioprocess performance has often been directed with the methanol regulated AOX1 promoter (P AOX1 ), and the constitutive GAP

Externí odkaz: https://doaj.org/article/65fec0020c894a709e03afcf4f3128d7

Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) P AOX1 -driven recombinant producer strains with different target gene dosage

Autor: Javier Garrigós-Martínez, Miguel Angel Nieto-Taype, Arnau Gasset-Franch, José Luis Montesinos-Seguí, Xavier Garcia-Ortega, Francisco Valero

Publikováno v: Microbial Cell Factories, Vol 18, Iss 1, Pp 1-15 (2019)

Abstract Background The P AOX1 -based expression system is the most widely used for producing recombinant proteins in the methylotrophic yeast Pichia pastoris (Komagataella phaffii). Despite relevant recent advances in regulation of the methanol util

Externí odkaz: https://doaj.org/article/74bc68089f074e718ee6a9073c01ca84

Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Autor: Ilya I. Tolstorukov, Miguel Angel Nieto-Taype, Francisco Valero, Merja Penttilä, Xavier Garcia-Ortega, Christian Schmid, José Luis Montesinos-Seguí, Knut Madden, Juha Tähtiharju, Kiira Vuoristo, Anton Glieder, Jaana Uusitalo, Pauliina Tukiainen, Javier Garrigós-Martínez

Publikováno v: Garrigós-Martínez, J, Vuoristo, K, Nieto-Taype, M A, Tähtiharju, J, Uusitalo, J, Tukiainen, P, Schmid, C, Tolstorukov, I, Madden, K, Penttilä, M, Montesinos-Seguí, J L, Valero, F, Glieder, A & Garcia-Ortega, X 2021, ' Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris ', Microbial Cell Factories, vol. 20, no. 1, 74 . https://doi.org/10.1186/s12934-021-01564-9
Universitat Autònoma de Barcelona
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-12 (2021)
Dipòsit Digital de Documents de la UAB
Microbial Cell Factories

Background Pichia pastoris is a powerful and broadly used host for recombinant protein production (RPP), where past bioprocess performance has often been directed with the methanol regulated AOX1 promoter (PAOX1), and the constitutive GAP promoter (P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2cc4373f7524246f16fe98260f683ce
https://cris.vtt.fi/en/publications/78573d8c-33b1-40f7-86f2-f4c89a4177c3

Fungal Laccases to Where and Where?

Autor: Hebatallah H. Abo Nahas, Safaa A. Mansour, Fatma Ahmed Abo Nouh, Daniela Landa-Acuña, Yousef H. Abo Nahas, Miguel Angel Nieto-Taype, Ahmed M. Abdel-Azeem

Publikováno v: Fungal Biology ISBN: 9783030856021

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9881dd80b9d57cf47537934c68ced56f
https://doi.org/10.1007/978-3-030-85603-8_6

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With

Autor: Miguel Angel, Nieto-Taype, Xavier, Garcia-Ortega, Joan, Albiol, José Luis, Montesinos-Seguí, Francisco, Valero

Publikováno v: Frontiers in Bioengineering and Biotechnology

The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is currently considered one of the most promising hosts for recombinant protein production (RPP) and metabolites due to the availability of several tools to efficiently regulate the reco

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9176ad9e54a63898ca7c5c062d1e3c3f
https://pubmed.ncbi.nlm.nih.gov/32671036