Zobrazeno 1 - 10
of 277
pro vyhledávání: '"Miguel Alcalde"'
Autor:
Alexander Karich, Fabian Salzsieder, Martin Kluge, Miguel Alcalde, René Ullrich, Martin Hofrichter
Publikováno v:
Applied Microbiology, Vol 3, Iss 3, Pp 826-840 (2023)
Eighteen short- to medium-chain monounsaturated fatty acids were screened for hydroxylation and epoxidation using eleven different peroxygenase preparations. Most of these unspecific peroxygenases (UPOs) are secreted by fungal species of the dark-spo
Externí odkaz:
https://doaj.org/article/509f14fb1aaa433b97479f6b63d6074b
Autor:
Pascal Püllmann, Anja Knorrscheidt, Judith Münch, Paul R. Palme, Wolfgang Hoehenwarter, Sylvestre Marillonnet, Miguel Alcalde, Bernhard Westermann, Martin J. Weissenborn
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-20 (2021)
Püllmann et al developed a modular Golden Gate-based secretion system, which enabled production and one-step purification of active fungal unspecific peroxygenases (UPOs) in yeast. Their system was applied to an enantioselective conversion on a prep
Externí odkaz:
https://doaj.org/article/f3c63e6d684b4ea8b0fbe616270d80cf
Publikováno v:
Frontiers in Catalysis, Vol 2 (2022)
The use of water-miscible organic co-solvents in biocatalysis is a simple procedure for obtaining higher enzymatic activities toward hydrophobic substrates. However, effects on activity and stability have to be carefully evaluated, also with regard t
Externí odkaz:
https://doaj.org/article/9685bc8c409344c295fbb80c15d602d0
Autor:
Mikel Dolz, Ivan Mateljak, Daniel Méndez-Sánchez, Israel Sánchez-Moreno, Patricia Gomez de Santos, Javier Viña-Gonzalez, Miguel Alcalde
Publikováno v:
Frontiers in Catalysis, Vol 2 (2022)
At present, the end-of-life management of thermoset composite epoxy resins is limited to incineration and landfill storage, highlighting the demand for the development of more sustainable measures. Due to their broad spectrum of C-H oxyfunctionalizat
Externí odkaz:
https://doaj.org/article/3771f9e76ef149748aaa0c65099b8ea3
Autor:
Javier Viña-Gonzalez, Miguel Alcalde
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 18, Iss , Pp 1800-1810 (2020)
Aryl-alcohol oxidase (AAO) is a fungal GMC flavoprotein secreted by white-rot fungi that supplies H2O2 to the ligninolytic consortium. This enzyme can oxidize a wide array of aromatic alcohols in a highly enantioselective manner, an important trait i
Externí odkaz:
https://doaj.org/article/05bcaaf42f2448288785cea95e27b9c1
Autor:
Patricia Molina-Espeja, Alejandro Beltran-Nogal, Maria Alejandra Alfuzzi, Victor Guallar, Miguel Alcalde
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
Externí odkaz:
https://doaj.org/article/38574d9819c24a70be09155322837afb
Autor:
Patricia Molina-Espeja, Alejandro Beltran-Nogal, Maria Alejandra Alfuzzi, Victor Guallar, Miguel Alcalde
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
Fungal unspecific peroxygenases (UPOs) are hybrid biocatalysts with peroxygenative activity that insert oxygen into non-activated compounds, while also possessing convergent peroxidative activity for one electron oxidation reactions. In several ligni
Externí odkaz:
https://doaj.org/article/37daa2c209774651bff0413dbf29dbfc
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 16, p 8934 (2022)
The routine generation of enzymes with completely new active sites is a major unsolved problem in protein engineering. Advances in this field have thus far been modest, perhaps due, at least in part, to the widespread use of modern natural proteins a
Externí odkaz:
https://doaj.org/article/637a3480d49d46e786369640ab828747
Autor:
Simon J. Freakley, Svenja Kochius, Jacqueline van Marwijk, Caryn Fenner, Richard J. Lewis, Kai Baldenius, Sarel S. Marais, Diederik J. Opperman, Susan T. L. Harrison, Miguel Alcalde, Martha S. Smit, Graham J. Hutchings
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
Continuous low-level supply or in situ generation of hydrogen peroxide (H2O2) is essential for the stability of unspecific peroxygenases. Here, the authors demonstrate that AuPd / TiO2 can generate sufficient H2O2 for the engineered unspecific heme-t
Externí odkaz:
https://doaj.org/article/191eddc66cb84147bad26f6e594b73cf
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Among the broad repertory of protein engineering methods that set out to improve stability, consensus design has proved to be a powerful strategy to stabilize enzymes without compromising their catalytic activity. Here, we have applied an in-house co
Externí odkaz:
https://doaj.org/article/69192d5580c04ee0be7655f55b6854ae