The Phosducin-Like Protein PhLP1 Is Essential for Gβγ Dimer Formation in Dictyostelium discoideum

Autor: Jaco C. Knol, Peter J.M. van Haastert, Antonie J. W. G. Visser, Mieke Blaauw, Ruchira Engel

Publikováno v: Molecular and Cellular Biology 25 (2005) 18
Molecular and Cellular Biology, 25(18), 8393-8400
Molecular and Cellular Biology, 25(18), 8393-8400. AMER SOC MICROBIOLOGY

Phosducin proteins are known to inhibit G protein-mediated signaling by sequestering G beta gamma subunits. However, Dictyostelium discoideum cells lacking the phosducin-like protein PhLP1 display defective rather than enhanced G protein signaling. H

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c74551072dc3881c871606e77169b34
https://hdl.handle.net/11370/ac9b190b-1db1-427d-8983-b94253a3b717

Structural evidence for dimerization-regulated activation of an integral membrane phospholipase

Autor: I. Ubarretxena-Belandia, H.J. Snijder, Bauke W. Dijkstra, Niek Dekker, Maarten R. Egmond, Mieke Blaauw, Hubertus M. Verheij, Kor H. Kalk

Publikováno v: Nature, 401(6754), 717-721. Nature Publishing Group

Dimerization is a biological regulatory mechanism employed by both soluble and membrane proteins'. However, there are few structural data on the factors that govern dimerization of membrane proteins. Outer membrane phospholipase A (OMPLA) is an integ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56cbc7cd509b1695195ea242c1521f6a
https://doi.org/10.1038/44890

The structure-function relationship of the lipases from Pseudomonas aeruginosa and Bacillus subtilis

Autor: Bauke W. Dijkstra, Onno Misset, Mieke Blaauw, Véronique Dartois, Karin Schanck, Karl-Erich Jaeger, Charles Colson, Stéphane Ransac, Gijs Gerritse, Ulrich Winkler, Emmanuel Lesuisse

Publikováno v: Protein Engineering, 7(4), 523-529

Within the BRIDGE T-project on lipases we investigate the structure-function relationships of the lipases from Bacillus subtilis and Pseudomonas aeruginosa. Construction of an overproducing Bacillus strain allowed the purification of > 100 mg lipase

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ead9f2ca31d1d3545d8163b289d69e6
https://doi.org/10.1093/protein/7.4.523

Crystallization and preliminary X-ray analysis of outer membrane phospholipase A from Escherichia coli

Autor: Niek Dekker, Hubertus M. Verheij, Mieke Blaauw, Kor H. Kalk, Bauke W. Dijkstra

Publikováno v: FEBS Letters, 373(1), 10-12. Wiley

The outer membrane phospholipase A (OMPLA) of Escherichia coli is one of the few integral outer membrane proteins displaying enzymatic activity, It is encoded as a mature protein of 269 amino acids preceded by a signal sequence of 20 amino acids. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6175548ba746f5c2836004879552d37f
https://doi.org/10.1016/0014-5793(95)01002-v

Crystallization and preliminary X-ray analysis of a lipase from Staphylococcus hyicus

Autor: Arend J. Slotboom, Mieke Blaauw, Hubertus M. Verheij, Jan-Willem P. Boots, Stéphane Ransac, Bauke W. Dijkstra

Publikováno v: Journal of Structural Biology, 114(2), 153-155. ACADEMIC PRESS INC ELSEVIER SCIENCE

Single cyrstals of a lipase from Staphylococcus hyicus have been obtained using a combination of 18 to 24% dimethylsulfoxide and 10% isopropanol as a precipitant. The crystals grow at 4 degrees C in 2-3 months. They belong to the orthorhombic space g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9090e86ca6fab41a67161e6719a6fb69
https://pubmed.ncbi.nlm.nih.gov/7612398