Polymer film-based microwell array platform for long-term culture and research of human bronchial organoids

Autor: Danielle Baptista, Zeinab Tahmasebi Birgani, Helene Widowski, Fiona Passanha, Vasili Stylianidis, Kèvin Knoops, Eva Gubbins, Cinta Iriondo, Kari-Pekka Skarp, Robbert J. Rottier, Tim G. Wolfs, Clemens van Blitterswijk, Vanessa LaPointe, Pamela Habibović, Niki L. Reynaert, Stefan Giselbrecht, Roman Truckenmüller

Publikováno v: Materials Today Bio, Vol 19, Iss , Pp 100603- (2023)

The culture of lung organoids relies on drops of basement membrane matrices. This comes with limitations, for example, concerning the microscopic monitoring and imaging of the organoids in the drops. Also, the culture technique is not easily compatib

Externí odkaz: https://doaj.org/article/d7c7a790a5b44159bbbc8155fb72fba8

3D Lung-on-Chip Model Based on Biomimetically Microcurved Culture Membranes

Autor: Danielle Baptista, Liliana Moreira Teixeira, David Barata, Zeinab Tahmasebi Birgani, Jasia King, Sander van Riet, Thijs Pasman, André A. Poot, Dimitrios Stamatialis, Robbert J. Rottier, Pieter S. Hiemstra, Aurélie Carlier, Clemens van Blitterswijk, Pamela Habibović, Stefan Giselbrecht, Roman Truckenmüller

Publikováno v: ACS Biomaterials Science and Engineering, 8(6), 2684-2699. AMER CHEMICAL SOC
ACS Biomaterials Science and Engineering, 8(6), 2684-2699. American Chemical Society
ACS Biomaterial Science and Engineering, 8(6), 2684-2699. American Chemical Society

A comparatively straightforward approach to accomplish more physiological realism in organ-on-a-chip (OoC) models is through substrate geometry. There is increasing evidence that the strongly, microscale curved surfaces that epithelial or endothelial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff600ff704c9acf587e39629f23ebd9b
https://doi.org/10.1021/acsbiomaterials.1c01463

Thin fluorinated polymer film microcavity arrays for 3D cell culture and label-free automated feature extraction

Autor: Clemens van Blitterswijk, Eva Gubbins, Pinak Samal, Stefan Giselbrecht, Roman Truckenmüller

Publikováno v: Biomaterials Science, 9(23), 7838-7850. Royal Society of Chemistry

There is an increasing need for automated label-free morphometric analysis using brightfield microscopy images of 3D cell culture systems. This requires automated feature detection which can be achieved by improving the image contrast, e.g. by reduci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92d235e8923fe99085c7c1887e181472
https://pubmed.ncbi.nlm.nih.gov/34671787

3D alveolar in vitro model based on epithelialized biomimetically curved culture membranes

Autor: A.A. Poot, L.S. Moreira Teixeira, Z. Tahmasebi Birgani, Danielle Baptista, Thijs Pasman, Stefan Giselbrecht, Pieter S. Hiemstra, Dimitris Stamatialis, Roman Truckenmüller, C.A. van Blitterswijk, Pamela Habibovic, R.J. Rottier, S. van Riet

Publikováno v: Biomaterials, 266:120436. ELSEVIER SCI LTD
Biomaterials
Biomaterials, 266:120436. Elsevier
Biomaterials, 266. ELSEVIER SCI LTD

There is increasing evidence that surface curvature at a near-cell-scale influences cell behaviour. Epithelial or endothelial cells lining small acinar or tubular body lumens, as those of the alveoli or blood vessels, experience such highly curved su

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e78826c6e6e816809f9c5e57e1acc972
https://hdl.handle.net/1887/3238566

3D Microcontact Printing for Combined Chemical and Topographical Patterning on Porous Cell Culture Membrane

Autor: Karin Friedel, Joerg Hampl, Patrick Mai, Sukhdeep Singh, Sebastian Haefner, Justyna Borowiec, Florian Ruther, Aldo R. Boccaccini, Dana Brauer, Andreas Schober, Liliana Liverani

Publikováno v: ACS Applied Materials & Interfaces. 10:22857-22865

Micrometer-scale biochemical or topographical patterning is commonly used to guide the cell attachment and growth, but the ability to combine these patterns into an integrated surface with defined chemical and geometrical characteristics still remain

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d82853d0208beed088acd38a0cf4f075
https://doi.org/10.1021/acsami.8b06585