Serum immunoglobulin or albumin binding single-domain antibodies that enable tailored half-life extension of biologics in multiple animal species

Autor: Michiel M. Harmsen, Bart Ackerschott, Hans de Smit

Publikováno v: Frontiers in Immunology, Vol 15 (2024)

Single-domain antibody fragments (sdAbs) can be isolated from heavy-chain-only antibodies that occur in camelids or the heavy chain of conventional antibodies, that also occur in camelids. Therapeutic application of sdAbs is often complicated by thei

Externí odkaz: https://doaj.org/article/6488e7aee6ec4cf38f6e1ab4547a1ec7

Mapping of foot-and-mouth disease virus antigenic sites recognized by single-domain antibodies reveals different 146S particle specific sites and particle flexibility

Autor: Michiel M. Harmsen, Haozhou Li, Shiqi Sun, Wim H. M. van der Poel, Aldo Dekker

Publikováno v: Frontiers in Veterinary Science, Vol 9 (2023)

Vaccination with intact (146S) foot-and-mouth disease virus (FMDV) particles is used to control FMD. However, 146S particles easily dissociate into stable pentameric 12S particles which are less immunogenic. We earlier isolated several single-domain

Externí odkaz: https://doaj.org/article/a0b5326e590f4289a2b5bc7c86079fc4

Novel Capsid-Specific Single-Domain Antibodies with Broad Foot-and-Mouth Disease Strain Recognition Reveal Differences in Antigenicity of Virions, Empty Capsids, and Virus-Like Particles

Autor: Haozhou Li, Aldo Dekker, Shiqi Sun, Alison Burman, Jeroen Kortekaas, Michiel M. Harmsen

Publikováno v: Vaccines, Vol 9, Iss 6, p 620 (2021)

Foot-and-mouth disease (FMD) vaccine efficacy is mainly determined by the content of intact virions (146S) and empty capsids (75S). Both particles may dissociate into 12S subunits upon vaccine manufacturing, formulation, and storage, reducing vaccine

Externí odkaz: https://doaj.org/article/5b6d952efc5646b29a052da3c4c1d48d

Isolation of Panels of Llama Single-Domain Antibody Fragments Binding All Nine Neuraminidase Subtypes of Influenza A Virus

Autor: Guus Koch, Herman van der Burg, Willem Bartelink, Juliette C. Blokker, Sylvia B. Pritz-Verschuren, Michiel M. Harmsen

Publikováno v: Antibodies, Vol 2, Iss 2, Pp 168-192 (2013)

Avian influenza A virus comprises sixteen hemagglutinin (HA) and nine neuraminidase (NA) subtypes (N1–N9). To isolate llama single-domain antibody fragments (VHHs) against all N subtypes, four llamas were immunized with mixtures of influenza viruse

Externí odkaz: https://doaj.org/article/f7d3ff2969c447449a663bc56ceedc49

Multimeric single-domain antibody complexes protect against bunyavirus infections

Autor: Frank Grosveld, Sara Rodriguez Conde, Paul J. Wichgers Schreur, Dorota Kozub, Jan Hellert, Kieran K Mistry, Erick Bermúdez-Méndez, Martin Beer, Pablo Guardado-Calvo, Félix A. Rey, Karen Brennan, Ziyan Deng, Michiel M. Harmsen, Olalekan Daramola, Sandra G. P. van de Water, Jeroen Kortekaas, Dubravka Drabek, Maiken Søndergaard Kristiansen, Andrea Aebischer, Lucien van Keulen, Kerstin Wernike

Publikováno v: eLife
eLife, eLife Sciences Publication, 2020, 9, pp.e52716. ⟨10.7554/eLife.52716⟩
eLife, 9:e52716. eLife Sciences Publications
eLife, 2020, 9, pp.e52716. ⟨10.7554/eLife.52716⟩
eLife, Vol 9 (2020)
eLife 9 (2020)
eLife, 9

International audience; The World Health Organization has included three bunyaviruses posing an increasing threat to human health on the Blueprint list of viruses likely to cause major epidemics and for which no, or insufficient countermeasures exist

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efa19d5508677dc13a105be694ea4493
https://hal-pasteur.archives-ouvertes.fr/pasteur-03207969/file/elife-52716-v1.pdf