Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Michelle M. Spiering"'
Autor:
Xiang Feng, Michelle M. Spiering, Ruda de Luna Almeida Santos, Stephen J. Benkovic, Huilin Li
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Abstract The T4 bacteriophage gp41 helicase and gp61 primase assemble into a primosome to couple DNA unwinding with RNA primer synthesis for DNA replication. How the primosome is assembled and how the primer length is defined are unclear. Here we rep
Externí odkaz:
https://doaj.org/article/65c6a9d0bd20462fb5a359252ed1232a
Publikováno v:
Viruses, Vol 7, Iss 6, Pp 3186-3200 (2015)
The T4 bacteriophage encodes eight proteins, which are sufficient to carry out coordinated leading and lagging strand DNA synthesis. These purified proteins have been used to reconstitute DNA synthesis in vitro and are a well-characterized model syst
Externí odkaz:
https://doaj.org/article/a17136f819464d4abcee7a0f63ea24a7
Autor:
Fangyuan Ding, Simona Cocco, Saurabh Raj, Maria Manosas, Thao Thi Thu Nguyen, Michelle M Spiering, David Bensimon, Jean-François Allemand, Vincent Croquette
Publikováno v:
Nucleic acids research, vol 50, iss 21
The hybridization kinetic of an oligonucleotide to its template is a fundamental step in many biological processes such as replication arrest, CRISPR recognition, DNA sequencing, DNA origami, etc. Although single kinetic descriptions exist for specia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f07e1c2ba79749434fcc0a0278c4bf37
https://doi.org/10.1093/nar/gkac1113
https://doi.org/10.1093/nar/gkac1113
Autor:
Stephen J. Benkovic, Henri Palacci, Xi Zhao, Vinita Yadav, Peter J. Butler, Ayusman Sen, Michelle M. Spiering, Michael K. Gilson, Henry Hess
Publikováno v:
Nature Chemistry. 10:311-317
Enzymatic catalysis is essential to cell survival. In many instances, enzymes that participate in reaction cascades have been shown to assemble into metabolons in response to the presence of the substrate for the first enzyme. However, what triggers
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5aa2aced333dcb019e679dfa737841b
https://doi.org/10.1038/nchem.2905
https://doi.org/10.1038/nchem.2905
Publikováno v:
Proceedings of the National Academy of Sciences. 114:5635-5640
The opposite strand polarity of duplex DNA necessitates that the leading strand is replicated continuously whereas the lagging strand is replicated in discrete segments known as Okazaki fragments. The lagging-strand polymerase sometimes recycles to b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0f923f51c0ecc333589406bd2c885d5
https://doi.org/10.1073/pnas.1620459114
https://doi.org/10.1073/pnas.1620459114
Autor:
Shengxi Chen, Manikandadas M. Madathil, Petro Yakovchuk, Stephen J. Benkovic, Michelle M. Spiering, Mohammad Parvez Alam, Sidney M. Hecht, Poulami Talukder, Basab Roy, Chandrabali Bhattacharya
Publikováno v:
Biochemistry. 54:7457-7469
Described herein are the syntheses and photophysical characterization of three novel cyanotryptophans, and their efficient incorporation into proteins as fluorescent probes. Photophysical characteristics indicated that each was significantly brighter
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d55be303211d8d24bce54b00be5bd35
https://doi.org/10.1021/acs.biochem.5b01085
https://doi.org/10.1021/acs.biochem.5b01085
Publikováno v:
Viruses
Viruses, Vol 7, Iss 6, Pp 3186-3200 (2015)
Viruses, Vol 7, Iss 6, Pp 3186-3200 (2015)
The T4 bacteriophage encodes eight proteins, which are sufficient to carry out coordinated leading and lagging strand DNA synthesis. These purified proteins have been used to reconstitute DNA synthesis in vitro and are a well-characterized model syst
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afe6a92152c95fd34bc952ad39fce2ed
http://europepmc.org/articles/PMC4488733
http://europepmc.org/articles/PMC4488733
The T4 replisome has provided a unique opportunity to investigate the intricacies of DNA replication. We present a comprehensive review of this system focusing on the following: its 8-protein composition, their individual and synergistic activities,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db2c4543c803ccf66f3cc1a8a9aa5924
https://europepmc.org/articles/PMC5682956/
https://europepmc.org/articles/PMC5682956/
Publikováno v:
Journal of Biological Chemistry. 288:20807-20816
In this study, we employed a circular replication substrate with a low priming site frequency (1 site/1.1 kb) to quantitatively examine the size distribution and formation pattern of Okazaki fragments. Replication reactions by the T4 replisome on thi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bb08d641c1b94f52ca99635c5f71ec44
https://doi.org/10.1074/jbc.m113.485961
https://doi.org/10.1074/jbc.m113.485961
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1804:1094-1112
Fast and accurate replication of DNA is accomplished by the interactions of multiple proteins in the dynamic DNA replisome. The DNA replisome effectively coordinates the leading and lagging strand synthesis of DNA. These complex, yet elegantly organi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3099d2cb63a62f28dd2643f70697e5dc
https://doi.org/10.1016/j.bbapap.2009.07.022
https://doi.org/10.1016/j.bbapap.2009.07.022