Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Michele C Darrow"'
Autor:
Maud Dumoux, Thomas Glen, Jake LR Smith, Elaine ML Ho, Luis MA Perdigão, Avery Pennington, Sven Klumpe, Neville BY Yee, David Andrew Farmer, Pui YA Lai, William Bowles, Ron Kelley, Jürgen M Plitzko, Liang Wu, Mark Basham, Daniel K Clare, C Alistair Siebert, Michele C Darrow, James H Naismith, Michael Grange
Publikováno v:
eLife, Vol 12 (2023)
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structur
Externí odkaz:
https://doaj.org/article/fbc6e73bd5344bb9801714368bad32dd
Publikováno v:
Wellcome Open Research, Vol 9 (2024)
The experimental limitations with optics observed in many microscopy and astronomy instruments result in detrimental effects for the imaging of objects. This can be generally described mathematically as a convolution of the real object image with the
Externí odkaz:
https://doaj.org/article/b5783015bf3748fe81f50853f7b30842
Autor:
Avery Pennington, Oliver N. F. King, Win Min Tun, Elaine M. L. Ho, Imanol Luengo, Michele C. Darrow, Mark Basham
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 10 (2022)
As sample preparation and imaging techniques have expanded and improved to include a variety of options for larger sized and numbers of samples, the bottleneck in volumetric imaging is now data analysis. Annotation and segmentation are both common, y
Externí odkaz:
https://doaj.org/article/2b55aef706e44f5fa6d4b6a9c534c36d
Publikováno v:
Microscopy and Microanalysis. 28:1238-1242
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fb92972729dbc89a8fe426ac6da91aaa
https://doi.org/10.1017/s1431927622005141
https://doi.org/10.1017/s1431927622005141
Autor:
Scott Gardner, Michele C. Darrow, Natalya Lukoyanova, Konstantinos Thalassinos, Helen R. Saibil
The bacterial chaperonin GroEL-GroES promotes protein folding through ATP-regulated cycles of substrate protein binding, encapsulation, and release. Here, we have used cryoEM to determine structures of GroEL, GroEL-ADP.BeF3, and GroEL-ADP.AlF3-GroES
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::24317f24acf8dba81f1ccde9da36ad3f
https://doi.org/10.1101/2023.05.29.542693
https://doi.org/10.1101/2023.05.29.542693
Autor:
Lucy M. Collinson, Carles Bosch, Anwen Bullen, Jemima J. Burden, Raffaella Carzaniga, Cheng Cheng, Michele C. Darrow, Georgina Fletcher, Errin Johnson, Kedar Narayan, Christopher J. Peddie, Martyn Winn, Charles Wood, Ardan Patwardhan, Gerard J. Kleywegt, Paul Verkade
Publikováno v:
Collinson, L M, Bosch, C, Bullen, A, Burden, J J, Carzaniga, R, Cheng, C, Darrow, M C, Fletcher, G, Johnson, E, Narayan, K, Peddie, C J, Winn, M, Wood, C, Patwardhan, A, Kleywegt, G J & Verkade, P 2023, ' Volume EM : a quiet revolution takes shape ', Nature Methods, vol. 20, no. 6, pp. 777-782 . https://doi.org/10.1038/s41592-023-01861-8
Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure of cells and tissues through continuous depths of at least 1 micrometer. A burgeoning grassroots community effort is fast building the profile and revealing t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d3913f95f04543d2a13dd1040ccd538
https://doi.org/10.1038/s41592-023-01861-8
https://doi.org/10.1038/s41592-023-01861-8
Autor:
Patricia Smith, Oliver NF King, Avery Pennington, Win Tun, Mark Basham, Martin L Jones, Lucy M Collinson, Michele C Darrow, Helen Spiers
Public participation in research, also known as citizen science, is being increasingly adopted for the analysis of biological volumetric data. Researchers working in this domain are applying online citizen science as a scalable distributed data analy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1499c47fe92a4109f98d8cccb57a5520
https://doi.org/10.21203/rs.3.rs-2639842/v1
https://doi.org/10.21203/rs.3.rs-2639842/v1
Autor:
Yehuda Halfon, Louie Aspinall, Joshua White, Isobel Jackson Hirst, Yiheng Wang, Michele C. Darrow, Stephen P. Muench, Rebecca F. Thompson
Publikováno v:
Faraday Discussions. 240:18-32
Cryo-electron microscopy (cryoEM) has been transformed over the last decade, with continual new hardware and software tools coming online, pushing the boundaries of what is possible and the nature and complexity of projects that can be undertaken. He
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aba53b2828a9283fb90baa34fac9739f
https://doi.org/10.1039/d2fd00129b
https://doi.org/10.1039/d2fd00129b
Autor:
Maud Dumoux, Thomas Glen, Jake LR Smith, Elaine ML Ho, Luis MA Perdigão, Avery Pennington, Sven Klumpe, Neville BY Yee, David Andrew Farmer, Pui YA Lai, William Bowles, Ron Kelley, Jürgen M Plitzko, Liang Wu, Mark Basham, Daniel K Clare, C Alistair Siebert, Michele C Darrow, James H Naismith, Michael Grange
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3d771b0ab21da6c07ba6e75f1abff440
https://doi.org/10.7554/elife.83623.sa2
https://doi.org/10.7554/elife.83623.sa2
Autor:
Casper Berger, Maud Dumoux, Thomas Glen, Neville B.-y. Yee, John M. Mitchels, Zuzana Patáková, Michele C. Darrow, James H. Naismith, Michael Grange
Publikováno v:
Nature Communications. 14
Structural biology studies inside cells and tissues require methods to thin vitrified specimens to electron transparency. Until now, focused ion beams based on gallium have been used. However, ion implantation, changes to surface chemistry and an ina
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b1ab8fa6436d1efadb3e4ceed68eb263
https://doi.org/10.1038/s41467-023-36372-9
https://doi.org/10.1038/s41467-023-36372-9