Výsledky vyhledávání - "Michel, Fromant"

DNA binding specificities of Escherichia coli Cas1–Cas2 integrase drive its recruitment at the CRISPR locus

Autor: Clara, Moch, Michel, Fromant, Sylvain, Blanquet, Pierre, Plateau

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2017, 45 (5), pp.2714-2723. ⟨10.1093/nar/gkw1309⟩

International audience; Prokaryotic adaptive immunity relies on the capture of fragments of invader DNA (protospacers) followed by their recombination at a dedicated acceptor DNA locus. This integrative mechanism, called adaptation, needs both Cas1 a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::7b04918c8d792275773b9db6629f7fc5
https://hal-polytechnique.archives-ouvertes.fr/hal-01961608/document

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Crystal Structure at 1.8 Å Resolution and Identification of Active Site Residues of Sulfolobus solfataricus Peptidyl-tRNA Hydrolase

Autor: Pierre Plateau, Christine Lazennec, Emmanuelle Schmitt, Sylvain Blanquet, Michel Fromant, Yves Mechulam

Publikováno v: Biochemistry
Biochemistry, American Chemical Society, 2005, 44 (11), pp.4294-301. ⟨10.1021/bi047711k⟩

International audience; The 3-D structure of the peptidyl-tRNA hydrolase from the archaea Sulfolobus solfataricus has been solved at 1.8 A resolution. Homologues of this enzyme are found in archaea and eucarya. Bacteria display a different type of pe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4703a3f3e6304d82c651ecb341e982e7
https://doi.org/10.1021/bi047711k

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Receptor Site for the 5‘-Phosphate of Elongator tRNAs Governs Substrate Selection by Peptidyl-tRNA Hydrolase

Autor: Emmanuelle Schmitt, Michel Fromant, Yves Mechulam, Pierre Plateau, Sylvain Blanquet

Publikováno v: Biochemistry. 38:4982-4987

Eubacterial peptidyl-tRNA hydrolase (PTH) recycles all N-blocked aminoacyl-tRNA molecules but initiator formyl-methionyl-tRNAfMet, the acceptor helix of which is characterized by a 1-72 mismatch. Positive selection by PTH of noninitiator tRNA molecul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49f7b7ec7aa303f9a0c4f021931aaf43
https://doi.org/10.1021/bi982657r

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Structure-Function Relationship of the Lrp-binding Region Upstream of lysU in Escherichia coli

Autor: Michel Fromant, Pierre Plateau, Philippe Dessen, Sylvain Blanquet, Florence Delort, Myriam Gazeau

Publikováno v: Journal of Molecular Biology. 241:378-389

In Escherichia coli, one of the two genes encoding lysyl-tRNA synthetase, lysU, belongs to the regulon controlled by the leucine-responsive regulatory protein (Lrp). To map the site of Lrp action, mutants escaping regulation in rich medium were gener

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::728a053ad0df6ef97ebc1e5199fdbb74
https://doi.org/10.1006/jmbi.1994.1514

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RNA-binding site of Escherichia coli peptidyl-tRNA hydrolase

Autor: Caroline Aubard, Sylvain Blanquet, Pierre Plateau, François Bontems, Laurent Giorgi, Michel Fromant

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2011, 286 (45), pp.39585-39594. ⟨10.1074/jbc.M111.281840⟩

International audience; In a cell, peptidyl-tRNA molecules that have prematurely dissociated from ribosomes need to be recycled. This work is achieved by an enzyme called peptidyl-tRNA hydrolase. To characterize the RNA-binding site of Escherichia co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba73b004b4acff80a7062be79352a1f0
https://pubmed.ncbi.nlm.nih.gov/21930710

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2D label-free imaging of resonant grating biochips in ultraviolet

Autor: Michel Fromant, Pierre Plateau, Jean-Luc Reverchon, Kristelle Bougot-Robin, Henri Benisty, Laurent Mugherli

Publikováno v: Optics Express
Optics Express, Optical Society of America-OSA Publishing, 2010, 8 (11), pp.11472. ⟨10.1364/OE.18.011472⟩

International audience; 2D images of label-free biochips exploiting resonant waveguide grating (RWG) are presented. They indicate sensitivities on the order of 1 pg/mm2 for proteins in air, and hence 10 pg/mm2 in water can be safely expected. A 320×

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a09a16f988191db4924635ae4afa90bc
https://hal-iogs.archives-ouvertes.fr/hal-00567020/file/oe-18-11-11472.pdf

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UV imaging of biochips based on resonant grating

Autor: Pierre Plateau, Henri Benisty, Jean-Luc Reverchon, Michel Fromant, Kristelle Bougot-Robin

Publikováno v: Proceedings of SPIE : Advanced Environmental, Chemical, and Biological Sensing Technologies VII
Advanced Environmental, Chemical, and Biological Sensing Technologies VII
Advanced Environmental, Chemical, and Biological Sensing Technologies VII, Apr 2010, Orlando, United States. ⟨10.1117/12.854239⟩

International audience; In the frame of biological threat, security systems require label free biochips for rapid detection. Biosensors enable to detect biological interactions, between probes localized at the surface of a chip, and targets present i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba4be27711d6fbc89506992e5e27f834
https://doi.org/10.1117/12.854239

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Biodetection of DNA and proteins using enhanced UV absorption by structuration of the chip surface

Autor: Laurent Mugherli, Jean-Luc Reverchon, Michel Fromant, Henri Benisty, Kristelle Robin

Publikováno v: SPIE Photonics West
SPIE Photonics West, Jan 2009, San Jose, United States. pp.718804, ⟨10.1117/12.808124⟩

DNA and protein absorption at 260 and 280 nm can be used to reveal theses species on a biochip UV image. A first study including the design and fabrication of UV reflective multilayer biochips designed for UV contrast enhancement (factor of 4.0) toge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3c9e21ed7ea4cd2899563e828856a4f
https://hal.archives-ouvertes.fr/hal-00573363

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Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces cerevisiae

Autor: Pierre Plateau, Michel Fromant, A Brevet, Sylvain Blanquet, J Chen

Publikováno v: Journal of Bacteriology. 173:5275-5279

An enzyme able to cleave dinucleoside triphosphates has been purified 3,750-fold from Saccharomyces cerevisiae. Contrary to the enzymes previously shown to catabolize Ap4A in yeast, this enzyme is a hydrolase rather than a phosphorylase. The dinucleo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2874aba0dfc129e742f231f2cbe276eb
https://doi.org/10.1128/jb.173.17.5275-5279.1991

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Detection of biological macromolecules on a biochip dedicated to UV specific absorption

Autor: Laurent Mugherli, Henri Benisty, Kristelle Robin, Pierre Plateau, Jean-Luc Reverchon, Michel Fromant

Publikováno v: Biosensors and Bioelectronics
Biosensors and Bioelectronics, Elsevier, 2008, 24 (6), pp.1585

International audience; This work describes an ultraviolet biosensing technique based on specific molecular absorption detected with a previously developed spectrally selective aluminum gallium nitride (AlGaN) based detector. Light absorption signal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c77c6cd6430395a7ff02993e871b4015
https://pubmed.ncbi.nlm.nih.gov/18823766

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